Studies of the Capacity of Bone‐Marrow Cells to Restore Erythropoiesis in Heavily Irradiated Rats

Blackett, N. M.; Roylance, Peter J.; Adams, K

doi:10.1111/j.1365-2141.1964.tb00722.x

Cited by 44 publications

(8 citation statements)

References 10 publications

(5 reference statements)

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“…On the other hand, as reflected by the stabilization of the thrombocyte and the granulocyte counts in a long term, these lineages also are able to attain a secondary steady state of cell production by compensating continuous damage to proliferating cells in the bone marrow. Such observations are congruent with those reported from the fundamental studies performed in rats and mice [2][3][4][5][6][7][8][9].…”

Section: Discussionsupporting

confidence: 92%

Chapter 6: Cell and cell system responses: Response of hemopoiesis in dogs to continuous low dose rate total body irradiation

et al. 1995

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Among the cytotoxic agents which particularly cause damage to cell renewal systems, ionizing radiation is one of the most effective ones since it leads to inactivation of all types of proliferating cells including resting stem cells. It is the aim of this paper to present the effects of continuous low dose rate total body irradiation (TBI) on hemopoiesis in dogs. The animals were exposed to gamma-rays from a 60-Co source, receiving a daily radiation dose of 0.0188 Gy for indefinite times. Sequential hematological studies performed included determinations of peripheral blood cell counts and of total cell numbers in standardized bone marrow samples, assessments of progenitor cells GM-CFC in the blood and bone marrow, and of colony-stimulating activity (CSA) in the serum. The lymphocytes, the thrombocytes and neutrophilic granulocytes uniformly showed early decreases within the first 200 to 500 days corresponding to cumulative radiation doses in the range up to 3.8 to 9 Gy, but remained stable at subnormal levels in the period up to 1,700 days of exposure. The GM-CFC numbers in bone marrow samples from the rib clearly showed a strong decrease within the first 150 days of exposure preceding the changes in the blood granulocyte concentration. A transient partial recovery of the GM-CFC was observed at later times between 700 and 1,200 days of exposure, followed by another decrease to extremely low values at cumulative doses in the range of 32 Gy.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 92%

Chapter 6: Cell and cell system responses: Response of hemopoiesis in dogs to continuous low dose rate total body irradiation

et al. 1995

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“…I)compared with those from normal mice(Fig. 2)might be taken to support the findings reported byBennett, Shearer, Upton and Cudkowicz (1964) on the stem-cell sparing effect of polycythaemia, but is in contrast to the fmdings ofBlackett et al (1964).…”

supporting

confidence: 86%

“…3. In one experiment there was no recovery of repopulating capacity at 24 hours after the first 50 rad, but a further depression, reminiscent of the 'post-irradiation dip' (Gurney et al, 1962;Till, 1963 ;Blackett et al, 1964) following hgher doses. I 82 D. D. Porteous and L. G. Lajtha During a regime of 50 rad daily an increasing number of mice died.…”

Section: Stem-cell Recovery After Irradiation 181mentioning

confidence: 99%

On Stem‐Cell Recovery after Irradiation

Porteous

Lajtha

1966

Br J Haematol

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“…Irrespective of the morphologic identity of the colony-forming cell, there is a question that this is the immediate precursor cell for each of the hematopoietic elements. The observations described herein, as well as those of megakaryocytic labeling (2) and the transplantation studies of Blackett, Roylance, and Adams (19) in sublethally irradiated animals, suggest that a more differentiated intermediate precursor may exist. Similarly the observations of Lewis and Trobaugh (20), although not interpreted as such, suggest to us a different immediate precursor cell for each cell line.…”

Section: Effect Of Vincristine and Erythropoietin On Bone Marrowmentioning

confidence: 83%

Regulation of erythropoiesis. 18. The effect of vincristine and erythropoietin on bone marrow.

Morse¹,

Stohlman²

1966

J. Clin. Invest.

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results to be reported herein appear to favor different immediate precursors and a more primitive pluripotential precursor cell. MethodsAdult female Sprague-Dawley rats weighing 150 to 170 g were given vincristine in a single intravenous dose of 0.3 mg per kg. This dose is just below the lethal range in this colony; 0.4 mg per kg resulted in a 90% mortality within 10 days. Rats, when transfused, were given washed, packed homologous red cells by tail vein injection 5 days before the start of the experiment. They received red cells in an amount equivalent to 3.5%o of the body weight. One hundred units of human urinary erythropoietin, prepared by alcohol fractionation (4), was given by subcutaneous injection. Blood samples were collected by cardiac puncture from anesthetized animals into Versenate. Peripheral blood values were measured by standard technics. Leukocyte differential counts were based on the evaluation of 200 cells in peripheral smears. Bone marrow smears were made from the split femur by a brush technic. The percentage of erythroid precursors was estimated from differential counts on 500 to 1,000 nucleated cells. The mitotic index was measured in these same preparations. Megakaryocytic differentials were based on evaluation of 100 megakaryocytes. Blood volume was measured from the dilution of intravenously administered 'Cr-labeled washed homologous red cells. Blood samples for red cell wFe incorporation were collected 20 hours after intravenous administration of plasmabound 'Fe. They were lysed with saponin and counted in a well type scintillation counter. The total iron incorporation was based on direct measurement of the blood volume. ResultsAfter the administration of vincristine to normal animals, the marrow smears revealed an accumulation of metaphase forms that approximated linearity for the first 4 hours. Extrapolation of this curve to 100% provided an estimate of -11 hours for the generation time of rat erythroid precursors. By 8 hours, 67% of erythroid precursors potentially capable of dividing and 23%o of granulocytic precursors potentially capable of dividing were in metaphase. No postmetaphase forms were 1241

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Studies of the Capacity of Bone‐Marrow Cells to Restore Erythropoiesis in Heavily Irradiated Rats

Cited by 44 publications

References 10 publications

Chapter 6: Cell and cell system responses: Response of hemopoiesis in dogs to continuous low dose rate total body irradiation

Chapter 6: Cell and cell system responses: Response of hemopoiesis in dogs to continuous low dose rate total body irradiation

On Stem‐Cell Recovery after Irradiation

Regulation of erythropoiesis. 18. The effect of vincristine and erythropoietin on bone marrow.

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