Studies of Penicillin G Acylase Immobilization Using Highly Porous Cellulose-Based Polymeric Membrane

Abstract

The different ionic molecules/compounds were used as a ligand for the immobilization of penicillin G acylase on the highly porous cellulose-based polymeric membrane having buffer flux 1,746 LMH (L m(-2) h(-1)) at 0.5 bar pressure. The immobilized enzyme activity around 250 U(App) was obtained with the ligand such as proline, tryptophan, casein acid hydrolysate, and brilliant green. Comparatively, proline showed less IMY% (percentage immobilization yield--58) but higher RTA% (percentage of activity retention--71) and specific activity (145 U(App) g(-1)). However, the crosslinked preparation of brilliant green obtained using glutaraldehyde showed 82 +/- 2.7% immobilized enzyme activity after the completion of successive five cycles. In comparison with the free enzyme, the enzyme immobilized on the brilliant green coupled membrane showed around 2.4-fold increase in K (m) value (47.4 mM) as well as similar optimum pH (7.2) and temperature (40 degrees C). The immobilized enzyme retained almost 50% activity after 107 days and 50 cycles of operation. Almost 50% decrease in buffer flux after enzyme immobilization was observed. At the end of the 30 cycles, flux pattern shows around 38% decrease in buffer flux however, after 16 cycles of operation flux moves closer towards the steady state.

Enzyme immobilization on/in polymeric membranes: status, challenges and perspectives in biocatalytic membrane reactors (BMRs)

“…67 Cibacron Blue F3GA, Brilliant green and Procion red are some dyes that have been successfully incorporated in membranes. [68][69][70][71] These dyes can easily be incorporated in cellulose based membranes due to the hydroxyl groups present in cellulose. 67 These hydroxyl groups interact with the chloride or fluoride atoms present on the triazinyl groups in the dyes.…”

“…This, however, is not a general practice as revealed by Table 3. 69 Entrapment of enzymes into membranes. Entrapment of enzymes has the advantage of wider applicability to many enzymes and relatively small changes in enzyme structure and function after immobilization.…”

Covalent Immobilization and Characterization of Penicillin G Acylase on Magnetic NiFe2O4 Nanorods Prepared via a Novel Rapid Combustion Process

“…6. The immobilized PGA retained over 70 % residual activity after being used 10 times, which was larger than that of penicillin G acylase on magnetic Fe 3 O 4 nanoparticles modified by ionic liquids 19 , and basically reached the level of penicillin G acylase on aldehyde-functionalized mesoporous silica-Fe 3 O 4 nanocomposites 20 . The high recycle relative activity revealed that the reusability of the immobilized PGA was better than that of free PGA.…”

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

“…Organic carriers include natural polymers and synthetic polymers. Natural macromolecule carriers mainly include cellulose, dextran, agarose, and chitosan and so on . Because of their wide sources, pollution‐free, renewability, and other advantages, they have attracted the attention of researchers.…”