“…In any case, while this effect should also occur in a DNA of more heterogeneous sequence and is consistent with the top three entries in Table III in which the probability of glucosylation is higher when the average minimal distance to the next HMC is larger, it fails, without further assumptions, to explain why glucosylation is limited to the 5-prime member of a sequence of HMC's (43). For these sequences, one would still invoke the above sequence rules.…”
Section: Basicpolypeptidessupporting
confidence: 68%
“…All four dinucleotides containing a purine and HMC are found to be partially glucosylated (42,43). All four dinucleotides containing a purine and HMC are found to be partially glucosylated (42,43).…”
Section: Basicpolypeptidesmentioning
confidence: 94%
“…The local sequences around unglucosylated residues in T2 DNA have been determined (42)(43)(44) using derived oligopyrimidine tracts and dinucleotides containing HMC. Some oligopyrimidines and their glucosyl-HMC contents are shown in Table III; these sequences account for 3~ of the unglucosylated HMC in T2 DNA (42); the rest may be in oligopyrimidine sequences terminated with T's, which were not examined.…”
“…In any case, while this effect should also occur in a DNA of more heterogeneous sequence and is consistent with the top three entries in Table III in which the probability of glucosylation is higher when the average minimal distance to the next HMC is larger, it fails, without further assumptions, to explain why glucosylation is limited to the 5-prime member of a sequence of HMC's (43). For these sequences, one would still invoke the above sequence rules.…”
Section: Basicpolypeptidessupporting
confidence: 68%
“…All four dinucleotides containing a purine and HMC are found to be partially glucosylated (42,43). All four dinucleotides containing a purine and HMC are found to be partially glucosylated (42,43).…”
Section: Basicpolypeptidesmentioning
confidence: 94%
“…The local sequences around unglucosylated residues in T2 DNA have been determined (42)(43)(44) using derived oligopyrimidine tracts and dinucleotides containing HMC. Some oligopyrimidines and their glucosyl-HMC contents are shown in Table III; these sequences account for 3~ of the unglucosylated HMC in T2 DNA (42); the rest may be in oligopyrimidine sequences terminated with T's, which were not examined.…”
“…The use of different phosphomonoesterases for the digests from the two strains was not expected to influence the results and the chromatograms gave no evidence for incomplete removal of phosphomonoester groups. Any appreciable diesterase activity in either type of enzyme preparation would have been clearly shown by the experiments of Hall & Sinsheimer (1963) or Burton & Petersen (1960) Petersen & Siebke, 1963). From our experience we prefer I Fig.…”
Section: Components Of Diphenylamine Digestsmentioning
confidence: 85%
“…* Elution of Dowex 1 (formate form) with 0-9N-formic acid thus resolves the nucleotides containing hydroxymethylcytosine as the only base into two groups according to whether the components are glucosylated or not. This system does not separate by chain length, since material from herring DNA gives one composite peak of cytosine nucleotides (Burton et al 1963).…”
Section: Components Of Diphenylamine Digestsmentioning
Phages T 2, T 4 and T 6 contain glucose attached as a-glucosyl, fl-glucosyl and a-gentiobiosyl groups to the hydroxymethylcytosine of the DNA (Lehman & Pratt, 1960; Kuno & Lehman, 1962). Although each type of phage DNA contains virtually the same amount of hydroxymethylcytosine, there are large differences in the total amounts of glucose and in the amounts of each of the three types of glucosylated hydroxymethylcytosine. In T 2-phage DNA, 24-28 % of the total hydroxymethylcytosine is not glucosylated; 5-6 % is substituted with oc-gentiobiosyl residues and the remainder bears oc-glucosyl groups (Lehman & Pratt, 1960). It has been shown (Kornberg, Zimmerman & Kornberg, 1961) that the hydroxymethylcytosine nucleotides are glucosylated after they have been incorporated into DNA, but it is not known whether the glucose is randomly distributed among these nucleotides or whether its attachment to a hydroxymethylcytosine residue is affected by the nature of the neighbouring nucleotides. We have investigated these possibilities by studying the nucleotide products obtained by digesting T 2-phage DNA with diphenylamine in aqueous formic acid (Burton & Petersen, 1960) and with pancreatic deoxyribonuclease. A preliminary note describing part of this work has been published (Lunt & Burton, 1962). MATERIALS AND METHODS Bacteriophage. Two wild-type strains of T2 phage were used: T2(K) phage was from
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