Phages T 2, T 4 and T 6 contain glucose attached as a-glucosyl, fl-glucosyl and a-gentiobiosyl groups to the hydroxymethylcytosine of the DNA (Lehman & Pratt, 1960; Kuno & Lehman, 1962). Although each type of phage DNA contains virtually the same amount of hydroxymethylcytosine, there are large differences in the total amounts of glucose and in the amounts of each of the three types of glucosylated hydroxymethylcytosine. In T 2-phage DNA, 24-28 % of the total hydroxymethylcytosine is not glucosylated; 5-6 % is substituted with oc-gentiobiosyl residues and the remainder bears oc-glucosyl groups (Lehman & Pratt, 1960). It has been shown (Kornberg, Zimmerman & Kornberg, 1961) that the hydroxymethylcytosine nucleotides are glucosylated after they have been incorporated into DNA, but it is not known whether the glucose is randomly distributed among these nucleotides or whether its attachment to a hydroxymethylcytosine residue is affected by the nature of the neighbouring nucleotides. We have investigated these possibilities by studying the nucleotide products obtained by digesting T 2-phage DNA with diphenylamine in aqueous formic acid (Burton & Petersen, 1960) and with pancreatic deoxyribonuclease. A preliminary note describing part of this work has been published (Lunt & Burton, 1962). MATERIALS AND METHODS Bacteriophage. Two wild-type strains of T2 phage were used: T2(K) phage was from
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