Studies of Lymphocytes and Their Derivative Cells in vitro

Studies of Lymphocytes and Their Derivative Cells in vitro

“…This correlates with the finding of increased amounts of ribosomes and endoplasmic reticulum in these cells (5,12,22), and an increase in glutamic dehydrogenase activity which links protein and carbohydrate metabolism (9).…”

Untersuchungen über Blut- und Gewebsmakrophagen

“…Bis heute ist jedoch noch nicht entschieden, ob diese Zellen aus Monozyten [3,36,40,41,58,64,69] oder Lymphozyten [4,23,24,25,26,31,32,33,34] entstehen und ob bei dieser Transformation die Anwesenheit von neutrophilen Granulozyten von Bedeutung ist [33,34], Für die eigenen Untersuchungen an Blutmakrophagen ver wendeten wir menschliches Venenblut, aus dem bei spontaner Sedi mentation eine Gesamtlcukozytcnfraktion und bei Anwendung der Trennmethode nach R abin o w itz [58] …”

PHA Short-Term Culture of Lymphocytes in Acute Leukemia during Remission

“…It seems well es tablished that, in order to prolong remission, therapy must be continued with a cyclic use of agents, that can interfere with vital processes of nor mal cell like lymphocytes. An important expression of biological activi ty of such cells is the blast transformation in presence of phytohaemagglutinin (PHA) [12] that causes morphological and biochemical modifi cations [2,4,7,8], A 72-hour culture of lymphocytes from normal sub jects, in presence of PHA, yields 60-70°/o of blast-like cells, 20-25%> of prolymphocytes, and only about 10%> of lymphocytes [1]. One of the most important biochemical modifications is the RNA synthesis in pro lymphocytes and in blast-like cells, which becomes an almost continuous process [10].…”