Studies of Lymphocytes and Their Derivative Cells in vitro

Gough, Julie E.; Mw, Elves

doi:10.1159/000209415

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…This may be due to accumulated stores, decreased requirement for arginine, or to formation of enzymes to synthesize the amino acid endogenously. The enzyme content of transformed cells is quantitatively and perhaps qualitatively increased in comparison to the small lymphocyte (29).…”

Section: Discussion"mentioning

confidence: 93%

Studies of Pplo Infection

Simberkoff

Thorbecke

Thomas

1969

The Journal of Experimental Medicine

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Inhibition of the mitotic reaction of lymphocytes to phytohemagglutinin (PHA) by certain species of mycoplasmas was described by Copperman and Morton (1). These investigators showed that suspensions of whole mycoplasmas, as well as aqueous extracts of frozen-thawed organisms (2), prevented the reaction while in contact with the cells; reactivity was restored when the lymphocytes were washed.In view of the ubiquity of mycoplasmas as contaminants of most tissue culture lines, as well as the possibility that these agents may play roles still unrecognized in the pathogenesis of disease, it is of importance to determine the mechanism underlying this phenomenon. Recent reports have suggested that the mycoplasmas inhibit lymphocyte transformation by competitive metabolism of arginine (3, 4, 5).The present report presents additional evidence that the arg~nlne dihydrolase enzyme system of mycoplasmas is responsible for lymphocyte inhibition. It will also be shown that the response of lymphocytes to antigenic st/mulation is similarly inhibited, and the in vitro production of antibody by lymph node explants is prevented. Material and MethodsPreparation of Mycoplasmal Extta~t.--Mycoplasmas were grown at 37°C in broth media, as previously described (6), supplemented with 0.5% arginine or 0.5% glucose. Titers of the organisms at the time of harvest were generally 10 ° CFU/ml. The mycoplasmas were sedimented by centrifugation and washed three times in 0.85% NaCI. They were then suspended

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Section: Discussion"mentioning

confidence: 93%

Studies of Pplo Infection

Simberkoff

Thorbecke

Thomas

1969

The Journal of Experimental Medicine

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“…Furthermore, the cellular events occurring during the transformation to macrophages must also be unaffected by X-rays in high doses. Among these changes are the appearance of increased amounts of hydrolytic and other enzymes (Gough and Elves, 1967). The macrophages appearing in these cultures are also unimpaired in their phagocytic capabilities.…”

Section: Discussionmentioning

confidence: 99%

Some observations on the life history of the macrophage

Elves

1970

The Journal of Pathology

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“…An entirely different category of effects of arginine might actually be postulated--namely effects analogous to those of polylysine and othe~ charged compounds in stimulation of micropinocytosis in macrophages (58). It is known that the arginine content of transformed lymphoid cells is higher than the unstimulated lymphocyte (59) and it is conceivable that this represents actively stimulated pinocytotic admission to the cytoplasm.…”

Section: Stimulation Of Normal Mouse Spleen Cells By Ppd After Addingmentioning

confidence: 99%

Cellular Recognition by Mouse Lymphocytes in Vitro

Adler

Takiguchi

Marsh

et al. 1970

The Journal of Experimental Medicine

135

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The lymphoid cells of man, rat, guinea pig, rabbit, and other species respond in vitro to a variety of specific and nonspecific stimuli. Metabolic and morphologic changes in a stimulated cell population lead to cell division and elaboration of several biologic potent effector substances into the medium (1, 2). Despite the obvious genetic and operational advantages o[ the laboratory mouse for in vitro studies of cellular recognition, use of this species has been limited by the short life span and lack of sustained stimulated cell division permitted by tissue culture conditions. Dutton (3) and Heumer and colleagues (4) have devised the most successful in vitro culture methods for mouse spleen cells; the former is now being widely used for the investigation of primary antibody synthesis in mouse spleen cell populations, and the latter for the study of cell to cell interaction. In other studies, cell division was shown to follow phytohemagglutinin (PHA) t stimulation of mouse lymphoid cells (5-7) and production of various effector substances followed alloantigen and PHA stimulation. (8-11) On the other hand, no culture medium capable of supporting extended viability and stimulation of mouse lymphoid cells has been described (12).This series of papers describes and characterizes in detail a technically simple and dependable method of cultivating mouse lymphoid cells in vitro, and describes the results of studies of stimulation and other events of cellular recognition which the technique permits. This paper reports methods and requirements

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Studies of Lymphocytes and Their Derivative Cells in vitro

Cited by 11 publications

References 9 publications

Studies of Pplo Infection

Studies of Pplo Infection

Some observations on the life history of the macrophage

Cellular Recognition by Mouse Lymphocytes in Vitro

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