Studies of interaction between terbium(III)-deferasirox and double helix DNA by spectral and electrochemical methods

Shaghaghi, Masoomeh; Dehghan, Gholamreza; Jouyban, Abolghasem; Sistani, Parisa; Arvin, Mitra

doi:10.1016/j.saa.2013.09.073

Cited by 35 publications

(6 citation statements)

References 40 publications

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“…We did not observe a decrease in the peak current, which is considered the main consequence of drug-DNA complex formation (Sirajuddin, Ali & Badshah, 2013). We also observed a significant increase in the peak current during the formation of the complex between DNA and terbium(III)-deferasirox (Shaghaghi et al, 2014) or 3,4-disubstituted 1,8-naphthalimide (Sharma et al, 2019). Thus, the increase in peak current, which is slight in the case of AV-153 salts, can be interpreted as a consequence of DNA and drug interaction.…”

Section: Ftir Spectroscopy Of Av-153 Salts and Their Complexes With Dnamentioning

confidence: 47%

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Ļeonova

Shvirksts

Borisovs

et al. 2020

PeerJ

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1,4-dihydropyridines (1,4-DHP) possess important biochemical and pharmacological properties, including antimutagenic and DNA-binding activity. The latter activity was first described for water-soluble 1,4-DHP with carboxylic group in position 4, the sodium salt of the 1,4-DHP derivative AV-153 among others. Some data show the modification of physicochemical properties and biological activities of organic compounds by metal ions that form the salts. We demonstrated the different affinity to DNA and DNA-protecting capacity of AV-153 salts, depending on the salt-forming ion (Na, K, Li, Rb, Ca, Mg). This study aimed to use different approaches to collate data on the DNA-binding mode of AV-153-Na and five other AV-153 salts. All the AV-153 salts in this study quenched the ethidium bromide and DNA complex fluorescence, which points to an intercalation binding mode. For some of them, the intercalation binding was confirmed using cyclic voltammetry and circular dichroism spectroscopy. It was shown that in vitro all AV-153 salts can interact with four DNA bases. The FTIR spectroscopy data showed the interaction of AV-153 salts with both DNA bases and phosphate groups. A preference for base interaction was observed as the AV-153 salts interacted mostly with G and C bases. However, the highest differences were detected in the spectral region assigned to phosphate groups, which might indicate either conformational changes of DNA molecule (B form to A or H form) or partial denaturation of the molecule. According to the UV/VIS spectroscopy data, the salts also interact with the human telomere repeat, both in guanine quadruplex (G4) and single-stranded form; Na and K salts manifested higher affinity to G4, Li and Rb –to single-stranded DNA.

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Section: Ftir Spectroscopy Of Av-153 Salts and Their Complexes With Dnamentioning

confidence: 47%

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Ļeonova

Shvirksts

Borisovs

et al. 2020

PeerJ

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“…Incubating DNA with drug results in a conformational change in the DNA, as the conversion from a more B‐like to a more C‐like state. The incubation of DNA with drug shows less perturbations at the bands, attributed to a typical non‐intercalative mode for all synthesized compounds, as shown in Figure …”

Section: Resultsmentioning

confidence: 79%

“…Furthermore, the peak potential shifted to a more negative value in the presence of DNA for both complexes that is typical of the non‐intercalation of synthesized complexes into DNA. On the other hand, if the interaction mode is non‐intercalative, the peak potential shifts to a more negative value, while intercalation‐binding results in the shifts to a more positive value …”

Section: Resultsmentioning

confidence: 99%

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Fekri

Salehi

Asadi

et al. 2017

Applied Organom Chemis

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Co(III) complexes of tridentate Schiff base ligands derived from N‐(2‐hydroxybenzylideneamino)benzamide (H2L1) and 2‐(2‐hydroxybenzylidene)hydrazine‐1‐carboxamide (H2L2) were synthesized and characterized using IR, Raman, 1H–NMR and UV–Vis spectroscopies. X‐ray single crystal structures of complexes 1 and 2 have also been determined, and it was indicated that these Co(III) complexes are in a distorted octahedral geometry. The cyclic voltammetry (CV) of the complexes indicates an irreversible redox behavior for both complexes 1 and 2. The antibacterial effects of the synthesized compounds have been tested by minimum inhibitory concentration and minimum bactericidal concentration methods, which suggested that the metal complexes exhibit better antibacterial effects than the ligands against Gram‐positive bacteria. The effects of the drug (drug = ligands and complexes) on bovine serum albumin (BSA) were examined using circular dichroism (CD) spectropolarimetry, and it was revealed that the BSA (BSA, as a carrier protein) secondary structure changed in the presence of the drug. Interaction of the drug with calf‐thymus DNA (CT‐DNA) was investigated by UV–Vis absorption, fluorescence emission, CV and CD spectroscopy. Binding constants were determined using UV–Vis absorption. The results indicated that the studied Schiff bases bind to DNA, with the hyperchromic effect and non‐intercalative mode in which the metal complexes are more effective than ligands. Furthermore, molecular docking simulation was used to obtain the energetic and binding sites for the interaction of the complexes with Mycobacterium tuberculosis enoyl‐acyl carrier protein reductase (InhA), and results showed that complex 1 has more binding energy.

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“…Actually, the experiments were carried out by keeping the concentration of DNA constant while varying the ZnO@SiO 2 @APTES/BB concentration. The band at 260 nm of DNA arises due to the π → π * transitions of DNA bases (Shaghaghi et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Zinc oxide nanoparticles coated with food colorant Brilliant black: Synthesis, characterization, and comparative DNA interaction and antibacterial activity studies

Monfared

Shahabadi

Hashemi

et al. 2021

J. Food Process. Preserv.

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In the present work, preparation and full characterization of the silica‐coated zinc oxide nanoparticles containing Brilliant black food colorant (denoted as ZnO@SiO2@APTES/BB) is reported. The interactions of Brilliant black food colorant and the nanoparticles containing food colorant (ZnO@SiO2@APTES/BB) with calf thymus‐DNA (ct‐DNA) were then investigated by UV‐vis and fluorescence spectroscopies. In the absorption spectra of ct‐DNA, the band centered at 260 nm exposes hyperchromism in the presence of Brilliant black and also ZnO@SiO2@APTES/BB. Moreover, the binding constant (Kb) of Brilliant black and ZnO@SiO2@APTES/BB with DNA were 2.8 × 103 M−1 and 1.87 × 104 M−1 at 25°C, respectively. From the fluorescence spectroscopy, the enthalpy and entropy changes (ΔH0 and ΔS0) for Brilliant black–DNA and ZnO@SiO2@APTMS/BB–DNA formation were determined. In addition, the competitive binding experiments revealed that the Brilliant black and ZnO@SiO2@APTMS/BB could release Hoechst 33,258 from grooves of DNA. Finally, the potential of antibacterial activities of ZnO nanoparticles, Brilliant black, and ZnO@SiO2@APTMS/BB samples was evaluated and compared with tetracycline antibiotic. It is interesting to note that Brilliant black showed excellent antibacterial activity against gram‐negative and gram‐positive bacteria as compared to known tetracycline antibiotic while the ZnO@SiO2@APTMS/BB acts as a weak antibacterial agent against gram‐negative and gram‐positive bacteria. Novelty impact statement The silica‐coated zinc oxide nanoparticles containing Brilliant black food colorant was synthesized and characterized. Brilliant black and the nanoparticles containing Brilliant black interact with calf thymus‐DNA as groove binders. Brilliant black showed excellent antibacterial activity in comparison to tetracycline drug and it is important for food preservation containing this colorant.

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Studies of interaction between terbium(III)-deferasirox and double helix DNA by spectral and electrochemical methods

Cited by 35 publications

References 40 publications

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Zinc oxide nanoparticles coated with food colorant Brilliant black: Synthesis, characterization, and comparative DNA interaction and antibacterial activity studies

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