Studies of Experimental Jembrana Disease in Bali Cattle. I. Transmission and Persistence of the Infectious Agent in Ruminants and Pigs, and Resistance of Recovered cattle to Re-infection

Soeharsono, S.; Hartaningsih, Nining; Soetrisno, M.; Kertayadnya, G.; Wilcox, G. E.

doi:10.1016/s0021-9975(08)80134-x

Cited by 47 publications

(35 citation statements)

References 2 publications

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“…This provides an explanation for the common occurrence of secondary infections which may lead to fatal issues in affected cattle (Dharma et al, 1991;Teuscher et al, 1982). The finding showed that no recurrence of disease is observed in recovered animals and importantly they resist to further infections for at least 22 months (Dharma et al, 1991;Soeharsono et al, 1990). This suggests that JDV infections induce a protective immunity and probably vaccination using denatured or attenuated viral particles, can be performed to limit the disease spread.…”

Section: Introductionmentioning

confidence: 84%

Vaccine against Jembrana Disease Virus Infection: A Summary Findings

Kusumawati¹,

Wanahari²,

Astuti³

et al. 2015

American Journal of Immunology

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Jembrana disease constitutes the main concern in cattle industry especially in Indonesia and Australia as it has caused important economic losses due to mortality of cattle. The pathology of the disease is unusual for a lentivirus infection as it is associated with a severe, often lethal disease syndrome and a short incubation period in cattle. For lack of efficient medical treatment of JDV-infected cattle, vaccination may therefore constitute an effective measure for the prevention or eradication of Jembrana disease. Up to date, only one type of vaccine has been reported and tested. It is based on inactivated, tissue-derived virus antigens (Tabanan/87 isolate, JDV TAB/87 ). This review summarize show current Jembrana disease vaccine was developed as well as evaluated and how these information might be useful in future vaccine design.

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Section: Introductionmentioning

confidence: 84%

Vaccine against Jembrana Disease Virus Infection: A Summary Findings

Kusumawati¹,

Wanahari²,

Astuti³

et al. 2015

American Journal of Immunology

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“…Distinguishing BIV-infection was only made feasible by using a BIVspecific monoclonal antibody that only recognizes the unique BIV GAG epitope, which is not shared by JDV [14]. During the acute phase, high titre of infectious JDV viral particles is found in plasma [18,20]. Viruses are also abundantly present in secreted fluids, namely milk and saliva.…”

Section: Discussionmentioning

confidence: 99%

“…As negative samples, we used total RNA extracted from blood JDV non-infected Bali cattle and Ongole cattle. It is worthwhile mentioning that JDV infections of Ongole cattle only induce mild clinical signs and the infectious agents persist for less than 9 months [18,19]. Therefore the diagnosed animal may be healthy, or too few viruses are found in the sampled blood, or they have been rapidly eliminated.…”

Section: Discussionmentioning

confidence: 99%

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Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

et al. 2015

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Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on conserved tm subunit of Jembrana disease virus env gene. The RT-LAMP conditions were optimized by varying the concentration of MgSO 4 , betaine, dNTP, and temperature as well as the time and duration of reaction. The primers sensitivity for JDV was confirmed. The method was able to detect env-tm gene dilution which contained 2 9 10 -15 g of template. Comparatively, the sensitivity of RT-LAMP/LFD was 100-fold more sensitive than reverse transcription-polymerase chain reaction. The primers specificity for JDV was also confirmed using positive and negative controls. This work also showed that virus detection could be done not only on total RNA extracted from blood but various organs could also be analyzed for the presence of JDV using RT-LAMP/LFD method. The whole process, including the LAMP reaction and the LFD hybridization step only lasts approximately 75 min. Results of analysis can be easily observed with naked eyes without addition of any chemical or further analysis. The combination of RT-LAMP with LFD makes the method a more suitable diagnostic tool in conditions where sophisticated and expensive equipments are not available for field investigations on Jembrana disease in Bali cattle.

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“…After short latent period, infected cattle show clinical signs of fever, lymphadenopathy and lymphopenia, at which stage high viral titres of 10 8 infectious units/mL are found in the plasma (Kusumawati et al, 2014b;Soeharsono et al, 1990;Soesanto et al, 1990). In fatal infection, death is attributed to multisystem involvement.…”

Section: Introductionmentioning

confidence: 99%

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

Kusumawati¹,

Wanahari²,

Tampubolon³

et al. 2015

American Journal of Biochemistry and Biotechnology

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Jembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three different detection methods based on genomic approach, i.e., reverse transcriptase-polymerase chain reaction (RT-PCR), reverse transcriptase-loop mediated isothermal amplification (RT-LAMP) and dot-blot hybridization to detect JDV. Utilization of pGEX-TM, a recombinant plasmid containing env-tm gene as a positive control showed that RT-LAMP is the most sensitive method compares the two others. It could detect template concentration as low as 10 −6 ng µL −1 or equivalent to 1.52×10 2 plasmid copy number, 100 and 10000 more sensitive than RT-PCR and dot-blot hybridization, respectively.

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Studies of Experimental Jembrana Disease in Bali Cattle. I. Transmission and Persistence of the Infectious Agent in Ruminants and Pigs, and Resistance of Recovered cattle to Re-infection

Cited by 47 publications

References 2 publications

Vaccine against Jembrana Disease Virus Infection: A Summary Findings

Vaccine against Jembrana Disease Virus Infection: A Summary Findings

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

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