Studies of cellular adhesiveness

Easty, G. C.; Easty, D.M.; Ambrose, E. J.

doi:10.1016/0014-4827(60)90062-8

Cited by 113 publications

(21 citation statements)

References 14 publications

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“…Included in these are reduced adhesion of cells to solid substrates (Easty, Easty & Ambrose, 1960;Weiss, 1963) and to each other (Moscona, 1952;Steinberg & Granger, 1966;Steinberg, 1967;Wiseman, Steinberg & Phillips, 1972); more rapid emigration of cells from explants (Simms & Stillman, 1937;Lefford, 1965); reduced binding of cytophilic antibodies (Kossard & Nelson, 1968), viruses (Zajac & Crowell, 1965), and sperm (Aketa, Onitake & Tsuzuki, 1972); altered carbohydrate uptake and metabolism (Kuo, Holmlund & Dill, 1966;Phillips, 1967); a release from postconfluence inhibition (Martz & Steinberg, 1972) of growth in vitro (Burger, 1970;Sefton & Rubin, 1970;Poste, 1971); increased deformability of the cell surface (Weiss, 1966); and animalization (Moore, 1952) and activation (Jensen, 1948) of unfertilized sea urchin eggs.…”

Section: Effects Of Proteolytie Enzymes On Living Cellsmentioning

confidence: 98%

On the recovery of adhesiveness by trypsin-dissociated cells

1973

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Summary.A sensitive method for assaying aggregation of dissociated cells has been developed which allows the determination of the mean number of cells per aggregate of a cell population. We have demonstrated that exposure of dissociated 6-or 7-day chick embryo neural retinal cells to trypsin in calcium-free solution renders them unable to aggregate for a half hour in stirred cell suspensions. Aggregation was noticeable first at 30 to 40 minutes and progressed to the formation of massive compact aggregates. Because the half-hour aggregation lag occurred both in the absence of serum and in medium reclaimed from aggregated preparations, the possibilities were excluded that it was due either to an inhibitor of aggregation in the serum, or was the time required for release into the medium of soluble aggregation-promoting materials emanating from the cells themselves. Cells dissociated by divalent cation withdrawal (Ca + +, Mg + +-free saline with EDTA) aggregated without a lag. The trypsin-induced lag does not appear to be the result of trypsin adsorbed to the surfaces of dissociated cells, as the lag is not abolished by addition of trypsin inhibitors to the aggregation medium. Microelectrophoresis of dissociated cells did not reveal changes in surface charge density during recovery from trypsinization. A variety of proteins and calcium ion, if present during trypsinization, protect the cells against the trypsin-induced aggregation lag. If the temperature was reduced from 37 to 6 ~ aggregation of fully adhesive cell populations came to a complete halt within 2 to 3 minutes. Aggregation resumed with a 5 to 10 minute delay when the temperature was returned to 37 ~ The rapidity of onset and reversal of inhibition of aggregation by low temperature treatment militates against the hypothesis that the low-temperature inhibition of aggregation acts by suppressing the synthesis of cell surface components necessary for adhesion. The abolition of the aggregation lag in trypsinized cells was also shown to be temperature-dependent; a 20-minute cold pulse administered in the middle of the lag period extended the length of the lag by exactly 20 minutes.Adhesiveness of cell surfaces is required so that cells can construct coherent tissues. A problem basic both to cell biology and to developmental

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Section: Effects Of Proteolytie Enzymes On Living Cellsmentioning

confidence: 98%

On the recovery of adhesiveness by trypsin-dissociated cells

1973

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“…Treatment with trypsin often gives rise to the appearance of a slimelike extraneous material embedding or entrapping cells (13,14) . After thorough washing, the cells are less adhesive than before trypsin treatment, a common experience first observed by Moscona (15) .…”

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confidence: 99%

A Study on the Mechanism of Intercellular Adhesion

Deman

Bruyneel

Mareel

1974

The Journal of Cell Biology

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Aggregation of suspended HeLa cells is increased on removal of cell surface sialic acid. Calcium ions promote aggregation whereas magnesium ions have no effect. The calcium effect is abolished by previous treatment of the cells with neuraminidase. Trypsinization of the HeLa cells followed by thorough washing diminishes the rate of mutual cell aggregation. Subsequent incubation with neuraminidase restores the aggregation rate to the original value before trypsin treatment. Cells which had acquired a greater tendency for aggregation after removal of peripheral sialic acid lose this property when subsequently treated with trypsin. Calcium ions have no aggregative effect on trypsinized cells. In contrast to HeLa cells, aggregation of human erythrocytes was not increased after treatment with neuraminidase or on addition of calcium. The results with HeLa cells are interpreted as follows: (a) Trypsin-releasable material confers adhesiveness upon the cells. (b) The adhesive property of this material is counteracted by the presence of cell surface sialic acids. (c) Calcium ions exert their effect by attenuating the adverse effect of sialic acid.

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“…Nevertheless, the number of cells immediately after the isolation and reseeding (at 0 h) cannot be compared accurately, because only the cells deposited on the culture dish were captured in the case of the trypsin reseeding, though all cells isolated by the impulsive force were captured after the isolation. In other words, we cannot count the cells disappeared in the reseeding process, though it is presumed that the cell membrane received chemical damage as a result of the trypsin treatment (Weiss 1963;Easty et al 1960). Previously, the cell detachment process from the source substrate due to trypsion treatment has been compared with that due to the impulsive force.…”

Section: Resultsmentioning

confidence: 98%

Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

Maezawa

Okano

Matsubara

et al. 2010

Biomed Microdevices

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When nerve growth factor (NGF) is interacted with PC12 cells derived from rat pheochromocytoma, they are partially differentiated into neuron-like cells with neurites. In this work, PC12 cells differentiated by NGF were selectively isolated using a localized impulsive force in a μm-scale area, which was generated by focusing an infrared femtosecond laser into a cell culture medium. In order to evaluate the ability of the isolation method, differentiated and undifferentiated cells were isolated and their morphological changes after the isolation were compared. In both cases, their neurites were once contracted and some of them gradually regenerated day by day. When differentiated cells were isolated, the percentage of differentiated cells with regenerated neurites, 6 h after the isolation, was about 3.3 times higher than that when undifferentiated ones were isolated. This result was compared with a control trypsin experiment. In the comparison, it was indicated that the same degree of cell function was maintained when the present isolation method was used.

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Studies of cellular adhesiveness

Cited by 113 publications

References 14 publications

On the recovery of adhesiveness by trypsin-dissociated cells

On the recovery of adhesiveness by trypsin-dissociated cells

A Study on the Mechanism of Intercellular Adhesion

Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

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