Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

Maezawa, Yasuyo; Okano, Kazunori; Matsubara, Mie; Masuhara, Hiroshi; Hosokawa, Yoichiroh

doi:10.1007/s10544-010-9476-4

Cited by 10 publications

(9 citation statements)

References 20 publications

(21 reference statements)

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“…By the quantification of the impulsive force, we were able to estimate intercellular breaking force of a leukocyte and an epithelial cell without modifying the culture system for the measurement. 7 We demonstrated that the impulsive force is practically available as an external force to manipulate the single cells 8,9 and to inject biomolecules into single cells. 10 For establishing applications, controllability of the impulsive force should be confirmed.…”

Section: Takanori Iino and Yoichiroh Hosokawamentioning

confidence: 99%

Controllability of femtosecond laser-induced impulse in water evaluated by local force measurement system using atomic force microscopy

Iino

Hosokawa

2012

Journal of Applied Physics

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A micro-sized impulse is generated in water by tightly focusing a femtosecond laser through an objective lens. Pulse energy dependence of the impulse was investigated for application as an external force to analyze single biological cells. The impulse proportionally increased between threshold (Eth) and 2 × Eth, and then it was saturated above 2 × Eth. In the proportional region, control precision of the impulse was 2.5 × 10−14 Ns, which was smaller than the uncertainty of the cell-cell adhesion force previously evaluated. This result indicates the impulse can be applied as a promising tool to estimate cell adhesion force.

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Section: Takanori Iino and Yoichiroh Hosokawamentioning

confidence: 99%

Controllability of femtosecond laser-induced impulse in water evaluated by local force measurement system using atomic force microscopy

Iino

Hosokawa

2012

Journal of Applied Physics

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“…By targeting the femtosecond laser in the vicinity of a target cell, this technique dissociates the adherent cell of interest from the culture scaffold (8)(9)(10)(11). This irradiation does not appear to damage cellular functions such as cell growth or cell differentiation (10,11).…”

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confidence: 99%

Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy

Hosokawa

Hagiyama

Iino

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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When a femtosecond laser pulse (fsLP) is focused through an objective lens into a culture medium, an impulsive force (fsLP-IF) is generated that propagates from the laser focal point (O f ) in a micron-sized space. This force can detach individual adherent cells without causing considerable cell damage. In this study, an fsLP-IF was reflected in the vibratory movement of an atomic force microscopy (AFM) cantilever. Based on the magnitude of the vibration and the geometrical relationship between O f and the cantilever, the fsLP-IF generated at O f was calculated as a unit of impulse [N-s]. This impulsive force broke adhesion molecule-mediated intercellular interactions in a manner that depended on the adhesion strength that was estimated by the cell aggregation assay. The force also broke the interactions between streptavidin-coated microspheres and a biotin-coated substrate with a measurement error of approximately 7%. These results suggest that fsLP-IF can be used to break intermolecular and intercellular interactions and estimate the adhesion strength. The fsLP-IF was used to break intercellular contacts in two biologically relevant cultures: a coculture of leukocytes seeded over on an endothelial cell monolayer, and a polarized monolayer culture of epithelial cells. The impulses needed to break leukocyte-endothelial and interepithelial interactions, which were calculated based on the geometrical relationship between O f and the adhesive interface, were on the order of 10 −13 and 10 −12 N-s, respectively. When the total impulse at O f is welldefined, fsLP-IF can be used to estimate the force required to break intercellular adhesions in a noncontact manner under biologically relevant conditions. adhesion molecule | biophysical measurement | epithelial adhesion | laser-induced impulsive force | leukocyte-endothelial adhesion

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“…Another trial which we introduce here is to elucidate effects of the impulsive force on animal cell differentiation using PC12 cells derived from rat pheochromocytoma. A schematic illustration of our experimental procedure 100 is given in Figure 36. When nerve growth factor is interacted with PC12 cells, they are partially differentiated into neuron like-cells with neuritis.…”

Section: Manipulation and Processing Of Living Cells By Femtosecond Lmentioning

confidence: 99%

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

Masuhara

2013

Bulletin of the Chemical Society of Japan

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Studies on laser ablation of polymer films, molecular crystals in solution, protein solution, and culture media containing living cells are summarized and considered. Dynamics and mechanism of laser ablation were systematically studied by utilizing time-resolved spectroscopy and imaging; femtosecondnanosecond transient absorption and emission spectroscopy, nanosecond shadowgraphy, nanosecondnanometer interferometry, femtosecond surface light scattering imaging. It was confirmed by integrating both data that primary processes of laser ablation can be well understood in the framework of Jablonski diagram. For nanosecond laser ablation of doped polymers, it was demonstrated that cyclic multiphoton absorption is an efficient photothermal conversion process leading to photothermal ablation. For femtosecond laser ablation of dye films, transient pressure mechanism was proposed indicating photomechanical ablation. As applications of laser ablation, nanoparticle preparation, protein crystallization, and manipulation of living cells are presented. Laser ablation of molecular crystals in poor solvent gives small fragments whose size are in a few tens nm. The fabricated nanocolloids are stable without adding detergents and their size was the smallest as nanoparticles produced by the top-down-method. Multiphoton laser ablation of water generates local impulsive force due to bubble formation, shockwave propagation, and local convection flow. The force triggers molecular and protein crystallization in their supersaturated solutions, whose mechanisms are described and considered. The impulsive force is also very useful for manipulating living cells and its high potential was confirmed by examining cell functions such as division, differentiation, death, and migration. Finally summary and future plan are presented.Studies on molecular spectroscopy and photochemistry introduced lasers very early in 1960's and used them as light sources for spectroscopic measurements as well as for inducing photochemical reactions. When chemists started to use pulsed lasers, they soon became aware that laser irradiation induced vaporization, decomposition, and fragmentation of materials and found that the irradiated surface was etched. The left patterns on the colored papers were practically used to confirm optical alignment in experimental setups before infrared (IR) viewer became popular. Later this laser-induced phenomenon was called laser ablation by Srinivasan, 1 and its systematic application was quickly developed to fabricate microstructures of various materials by electronics engineers and to microsurgery by medical doctors. Laser ablation is made possible by applying pulsed lasers and never brought out by weak continuous wave (CW) laser even when its irradiation continues for 1 year. Laser ablation is one of representative nonlinear photochemical phenomena and has a threshold with respect with laser fluence. Initially most of laser ablation studies were carried out for metals, semiconductors, ceramics, and glasses, 2 while organic materi...

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Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

Cited by 10 publications

References 20 publications

Controllability of femtosecond laser-induced impulse in water evaluated by local force measurement system using atomic force microscopy

Controllability of femtosecond laser-induced impulse in water evaluated by local force measurement system using atomic force microscopy

Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy

Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications

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