Studies of a Recombinant which Inherited the Haemagglutinin from the Human Influenza Virus A/Hong Kong/1/68 (H3N2) and Other Genes from Influenza Virus A/duck/Ukraine/1/63 (H3N8)

Ghendon, Y. Z.; Klimov, Alexander; Ginzburg, V. P.

doi:10.1099/0022-1317-65-1-165

Cited by 4 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…b 3H-labelled RNA of A/Duck/Hoshimin/014/78 (1 and 6), reassortants R 1 (2) (2 and 9), R 2 (6) 3 (3 and 8) and R (8) (4 and 7), and A/USSR/90/ 77 (5 and 10) was annealed with an excess of virion RNA of A/USSR/90/77 (1, 2, 3, 4, and 5) or A/Duck/Hoshimin/014/78 (6, 7, 8, 9, and 10). Triangles indicate the position of homoduplexes in reassortant RNA samples (1,3,5,7,9, and 11) and 48 hours (2,4,6,8, and 10) post infection thesis can be revealed no sooner than the majority (or, at least, a large part) of the cell population is infected. At 31 °C this condition was achieved by 48 h post infection with A/USSR/90/77, R 3 or R (8); in the cells infected with R 1 (2) or R 2 (6) 3, only the synthesis of cell proteins was registered (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…The properties of the hemagglutinin also seem to be of importance. The hemagglutinins of avian and human viruses recognize different kinds of cell receptors [1,11]; the substitution of HA gene suppresses the ability of avian viruses to replicate in the avian host [3,9].…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, the HA gene is considered to be important for the virus reproduction in avian hosts. The replacement of the HA gene in an avian influenza virus with the HA gene of a human strain resulted in a strong suppression of the ability of the virus to replicate in the avian host [3,9]. The suppression could be reversed by a point mutation in HA gene [10,12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human-avian influenza virus reassortants: effect of reassortment pattern on multi-cycle reproduction in MDCK cells

Каверин

Руднева

Smirnov

et al. 1988

Archives of Virology

View full text Add to dashboard Cite

Human-avian influenza reassortants possessing the HA gene of the avian parent virus were tested for their ability to replicate in MDCK cells at 37 degrees C and 31 degrees C. Both avian parent viruses, A/Duck/Ukraine/1/63 (H3N8) and A/Duck/Hoshimin/014/78 (H5N3) induced an efficient multi-cycle infection at 37 degrees C, but replicated poorly at 31 degrees C, whereas the human parent virus, MDCK-adapted variant of A/USSR/90/77 (H1N1) strain, replicated efficiently at both temperatures. The reassortant clone possessing the HA gene of A/Duck/Ukraine/1/63 virus and the other 7 genes of A/USSR/90/77 virus replicated at both temperatures almost as efficiently as the human parent virus. Among the reassortants between A/Duck/Hoshimin/014/78 and A/USSR/90/77, the clones possessing the HA and NA genes of the avian strain, or the HA, NA, NP, and NS genes of the avian strain, and the other genes of the human parent virus, replicated poorly at both temperatures, especially at 31 degrees C, whereas the reassortant possessing the HA, NA, and M genes of the avian virus replicated at both temperatures fairly efficiently. The results are discussed in connection with the limitations imposed by different genes upon avian influenza viruses' ability to replicate in mammalian cells.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human-avian influenza virus reassortants: effect of reassortment pattern on multi-cycle reproduction in MDCK cells

Каверин

Руднева

Smirnov

et al. 1988

Archives of Virology

View full text Add to dashboard Cite

show abstract

“…In these studies, adapted virus often showed a change in receptor-binding preference and a reduction in NA stalk length, consistent with what we observed in Quail/CA12. The higher binding preference for α2,6-linked sialic acids in the HA of Quail/CA12 likely altered its ability to infect duck-origin cells, and enhanced its ability to infect chickens [20, 27, 28]. Other molecular changes such as the K151T mutation (which results in the introduction of a putative glycosylation sequon in the 130-loop) [29, 30], and the DG156/157GE and A158E on the top and the edge of the globular head, respectively [24, 25] were also observed in laboratory -adaptation experiments and could have occurred to compensate for the reduced NA activity due to a shorter stalk [29–31].…”

Section: Discussionmentioning

confidence: 99%

Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

et al. 2014

View full text Add to dashboard Cite

The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEKRRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.

show abstract

Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus

Nerome¹,

Yoshioka

Sakamoto

et al. 1985

Archives of Virology

View full text Add to dashboard Cite

A recombinant (H1N2, formerly Hsw 1N2), A/swine/Ehime/1/80 was found to possess antigenic, biological and genomic characteristics different from those of a previous A/swine/Kanagawa/2/78 (H1N2) strain. Five monoclonal antibodies to A/NJ/8/76 definitely differentiated the hemagglutinin molecules of the former virus from the latter, showing that these viruses differed, at least, at two antigenic determinants. Neuraminidase-inhibition tests with monoclonal antibodies to different H2N2 and H3N2 viruses revealed that the A/swine/Ehime/1/80 strain contained a neuraminidase very similar to that of the late human Asian (H2N2) and the earliest Hong Kong (H3N2) viruses. Growth comparison of swine and human isolates indicated that A/swine/Ehime/1/80 and A/swine/Shizuoka/1/78 (H1N1) failed to grow at 42 degrees C, while A/swine/Kanagawa/2/78 and its possible parental virus, A/swine/Kanagawa/4/78 (H1N1) replicated efficiently at this stringent temperature. These results revealed that the viruses having growth characteristics similar to those of avian influenza virus were present in the swine population. RNA analysis by oligonucleotide mapping suggested that A/swine/Ehime/1/80 may be a recombinant between A/swine/Shizuoka/1/78-like and A/Aichi/2/68 (H3N2)-like viruses. To further determine the gene constellation of this recombinant virus, DNA-RNA hybridization was performed by using DNA segments complementary for swine (H1N1) virus RNA and the entire RNAs of three viruses. The molecular hybridization could define the genomic composition of the recombinant, indicating that only the neuraminidase gene of this virus is derived from the earliest Hong Kong (H3N2)-like virus and remaining seven genes from swine (H1N1) virus.

show abstract

Studies of a Recombinant which Inherited the Haemagglutinin from the Human Influenza Virus A/Hong Kong/1/68 (H3N2) and Other Genes from Influenza Virus A/duck/Ukraine/1/63 (H3N8)

Cited by 4 publications

References 18 publications

Human-avian influenza virus reassortants: effect of reassortment pattern on multi-cycle reproduction in MDCK cells

Human-avian influenza virus reassortants: effect of reassortment pattern on multi-cycle reproduction in MDCK cells

Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus

Contact Info

Product

Resources

About