Studies &lt;i&gt;in vivo&lt;/i&gt; and &lt;i&gt;in vitro&lt;/i&gt; of the Transfer of Rat IgG and Rat Albumen Across the Intestinal Walls of Young Rats

Jones, Richard E.

doi:10.1159/000240653

Cited by 9 publications

(9 citation statements)

References 14 publications

(19 reference statements)

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“…It is probable that albumin is absorbed via another route than IgG, perhaps via nonselectivc endocytosis. as suggested by Wild [29], This type of protein uptake appears to continue even in adult life [16,26,30], but the rate might decline with growth of the rat [11,14]. The ability to absorb IgG via spe cific receptors disappears at an age of about 21 days in the rat [2,22], and from this age the absorption of macromoleculcs into the serum is supposed to be maintained via basal nonselective endocytosis.…”

Section: Discussionmentioning

confidence: 98%

Proteolytic Activity as a Regulator of the Transmission of Orally Fed Proteins from the Gut to the Blood Serum in the Suckling Rat

Telemo¹,

Weström²,

Karlsson³

1982

Neonatology

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14-day-old rats were orally fed with porcine colostrum or serum having a low or high activity of protease inhibitors (i.e., sow colostrum trypsin-chymotrypsin inhibitor or soybean trypsin inhibitor). The uptake of undigested porcine albumin and IgG to the blood serum of these rats was studied 4 h after feeding. The effect of the exclusion of proteases to the intestinal lumen by means of pancreatic duct ligation prior to feeding was also studied. The results from these feeding experiments in the presence of protease inhibitors or in the absence of pancreatic proteases agreed well. It was concluded that for the suckling rat the intraluminal proteolytic activity in the gastrointestinal tract is a regulator for nonselective protein uptake, as represented by albumin, while the selective absorption of IgG was unaffected.

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Section: Discussionmentioning

confidence: 98%

Proteolytic Activity as a Regulator of the Transmission of Orally Fed Proteins from the Gut to the Blood Serum in the Suckling Rat

Telemo¹,

Weström²,

Karlsson³

1982

Neonatology

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“…Diese Annahme wird durch Untersuchungen von Jones [26] mit markiertem Ratten-Albumin bestfitigt, in denen er nachwies, dab es zwischen dem 3. und 14. Hierauf deutet der charakteristische, zeitabh~ngige Konzentrationsverlauf einiger Serumfraktionen z.B.…”

Section: Diskussionunclassified

“…Die Resorbtion des IgG wird bei Ratten durch die basischen Aminos~iuren Arginin und Lysin stimuliert [6]. Nach den Untersuchungen yon Jones [26] mit ~31K-markiertem R a t t e n -I g G ist das M a x i m u m der Resorbtion des I g G bereits am 14. Tag erreicht.…”

Section: Diskussionunclassified

Die Serumproteine der wachsenden Gunn-Ratte

1981

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Microprocesses of quantitative agarose gel electrophoresis and biuret total protein evaluation were used for the quantitative estimation of the serum proteins of homozygous and heterozygous Gunn rats. For the analyses carried out in the course of hyperbilirubinemia, 6 microliter of serum were necessary in each case, of which 50-80 microgram were used for electrophoresis. The quantitative evaluation of the phoretogram curves plotted at two wavelengths was carried out with the aid of an analog computer. Eleven fractions were quantitatively covered by the process. Age-related concentration shifts were observed for a number of fractions, especially for albumin, transferrin, and IgG. The results obtained from a large number of animals in longitudinal and cross-sectional examination corroborate various reports found in the relevant literature.

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“…During transmission, the macro molecules are protected from proteolytic degradation by protease inhibitors present in colostrum [Laskowski et al, 1957;Carlsson et al, 1980;Westrom et al, 1982]. In the newborn pig, this results in an effective transmission of immunoglobulins and other macromolecules from colostrum to the blood [Jensen and Pedersen, 1979;Westrom et al, 1985], In neonatal rodents, immunoglobulins (IgG) are endocytosed and selectively trans mitted to the blood after binding to specific Fc receptors on the enterocytes in the proxi mal small intestine [Rodewald, 1973;Mor ris, 1976;MacKenzie, 1984], Other macro molecules are transmitted simultaneously in small amounts [Jones, 1974;Morris et al, 1981;Telemo et al, 1982]. In the rat, this macromolecular transmission lasts 3 weeks.…”

Section: Introductionmentioning

confidence: 99%

Intestinal Macromolecular Transmission in the Young Rat: Influence of Protease Inhibitors during Development

Telemo

Weström²,

Ekström³

et al. 1987

Neonatology

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Intestinal macromolecular transmission in young rats of 10, 14, 18, 22 and 30 days of age was measured as the blood serum levels of markers 6 h after oral feeding of a solution containing bovine IgG (BIgG), bovine serum albumin (BSA) and fluorescein-isothiocyanate-labeled dextran 70,000 (FITC-D), either alone (controls) or with soybean trypsin inhibitor (SBTI) or swine colostrum trypsin inhibitor (SCTI). In the 10- and 14-day-old rats, transmission of all three macromolecular markers was high, with a preference for IgG. Transmission was greatly reduced by the age of 18 days and totally arrested for the protein markers at 22 days, with a low transmission of FITC-D remaining at 30 days of age. Addition of either of the two protease inhibitors significantly elevated the transmission of the protein markers in the rats aged 10, 14 and 18 days, while the transmission of the protease-independent marker FITC-D was unaffected. From 14 days of age, the rats have a functioning intestinal proteolysis, since only small amounts of marker proteins were left in the gut lumen 6 h after feeding, and since the addition of protease inhibitors resulted in increased amounts of undegraded proteins intraluminally. The results indicate that the increase of intraluminal proteolytic activity during development and the presence of protease inhibitors in the food are of importance for the intestinal transmission of undegraded proteins in the young rat. The Fc receptor for IgG in the enterocyte is not sufficient to maintain an optimal transmission of IgG, since the intraluminal proteolytic activity also appears to be of importance.

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Studies <i>in vivo</i> and <i>in vitro</i> of the Transfer of Rat IgG and Rat Albumen Across the Intestinal Walls of Young Rats

Cited by 9 publications

References 14 publications

Proteolytic Activity as a Regulator of the Transmission of Orally Fed Proteins from the Gut to the Blood Serum in the Suckling Rat

Proteolytic Activity as a Regulator of the Transmission of Orally Fed Proteins from the Gut to the Blood Serum in the Suckling Rat

Die Serumproteine der wachsenden Gunn-Ratte

Intestinal Macromolecular Transmission in the Young Rat: Influence of Protease Inhibitors during Development

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