Studies in penicillin hypersensitivity

Siegel, Bernard B.; Coleman, Monroe

doi:10.1016/0021-8707(57)90132-6

Cited by 14 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The test we developed allowed the correct identification of all the selected drugs tested, including sulfamethoxazole, probenecid and procainamide for which metabolism is needed. Penicillin G, another drug frequently associated with hypersensitivity reactions (Siegel and Coleman, 1957 ), was previously tested, and found to be able to induced a dose-related release of IL-8 following 48 h of exposure (Mitjans et al, 2008 ).…”

Section: Potential In Vitro Pre-clinical Tests To mentioning

confidence: 99%

In vitro Models to Evaluate Drug-Induced Hypersensitivity: Potential Test Based on Activation of Dendritic Cells

et al. 2016

View full text Add to dashboard Cite

Hypersensitivity drug reactions (HDRs) are the adverse effect of pharmaceuticals that clinically resemble allergy. HDRs account for approximately 1/6 of drug-induced adverse effects, and include immune-mediated (“allergic”) and non-immune-mediated (“pseudo allergic”) reactions. In recent years, the severe and unpredicted drug adverse events clearly indicate that the immune system can be a critical target of drugs. Enhanced prediction in preclinical safety evaluation is, therefore, crucial. Nowadays, there are no validated in vitro or in vivo methods to screen the sensitizing potential of drugs in the pre-clinical phase. The problem of non-predictability of immunologically-based hypersensitivity reactions is related to the lack of appropriate experimental models rather than to the lack of -understanding of the adverse phenomenon. We recently established experimental conditions and markers to correctly identify drug associated with in vivo hypersensitivity reactions using THP-1 cells and IL-8 production, CD86 and CD54 expression. The proposed in vitro method benefits from a rationalistic approach with the idea that allergenic drugs share with chemical allergens common mechanisms of cell activation. This assay can be easily incorporated into drug development for hazard identification of drugs, which may have the potential to cause in vivo hypersensitivity reactions. The purpose of this review is to assess the state of the art of in vitro models to assess the allergenic potential of drugs based on the activation of dendritic cells.

show abstract

Section: Potential In Vitro Pre-clinical Tests To mentioning

confidence: 99%

In vitro Models to Evaluate Drug-Induced Hypersensitivity: Potential Test Based on Activation of Dendritic Cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Thus, penicillin allergies have given positive patch tests to penicillin even after it has heen inactivated by penicillinase (Goldman, Friend & Mason 1946). Furthermore, in passive transfer tests with sera from penicillin allergies it has been observed that positive reactions could not be elicited by proeainepenicillin unless the penieillin preparation had been heated for some time (Siegel & Coleman 1957).…”

Section: Introductionmentioning

confidence: 99%

“…Some patients seem to be extremely sensitive to penieillin, intraeutaneous tests usinf raetions of i unit of benzylpenieillin having in many eases elieited systemic allergie reaetion (Coleman & Szvineford 1956, Bierlein 1956, Pedersen-Bjergaard 1967a. Therefore, warnings have been sounded against routine eutaneous testing of penieillin allergies with penieillin (Siegel & Coleman 1957). However, eutaneous tests with penieillin are still bemg done in tbe event of a suspieion of penieillin allergy, m partieular for researeh purposes.…”

Section: Introductionmentioning

confidence: 99%