Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani

Abstract

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.

Karakteristik Morfologi Hati Ayam Cemani (Gallus gallus domesticus)

“…Penelitian mengenai morfofungsi sistem organ ayam cemani belum banyak dilaporkan. Beberapa penelitian mengenai ayam cemani terdahulu yang sudah dilaporkan antara lain mengenai studi distribusi GLUT4 pada otot skelet [10] , pewarisan karakter fenotip [4] , perbandingan genom [11] , karakteristik bakteri asam laktat yang diisolasi pada saluran pencernaan [12] , dan kualitas daging dan struktur histologi otot dada dan paha [13] . Namun, penelitian mengenai morfologi saluran pencernaan khususnya hati pada ayam cemani belum pernah dilaporkan.…”

Literature Review: Antidiabetic Activities and Phytochemical Screening of Broccoli Plants (Brassica oleracea L. Var. italica)

“…GLUT4 (Glucose transporter 4), a protein encoded by the GLUT4 gene, is a glucose transporter that is regulated by insulin. GLUT4 is found in the body, especially in adipose and striated tissue [30]. In the state of insulin resistance, tissues that are involved in the process of glucose homeostasis as well as those that are sensitive to insulin (eg liver, muscle, adipose tissue) no longer respond well to insulin.…”