In this work, real power loss has done through hybridized Tree-seed algorithm. Sine-cosine algorithm which has been combined with Tree-seed algorithm (HTS) is projected to solve the problem. Tree-seed algorithm is based on the relationship between trees and seeds. And Sine Cosine Algorithm is based on the functions of Sine and Cosine; it stimulates the leader variable agent solutions towards the most excellent solution. In this work, seed engendering mechanism has been enhanced through adaptive mode and with reference to the iterations a linearly (k) varying mechanism has been implemented to perk up the exploration and exploitation. With considering voltage stability index proposed hybridized Tree-seed algorithm (HTS) is tested in IEEE 30, bus system. Then, the Proposed hybridized Tree-seed algorithm has been tested in standard IEEE 14, 30, 57, 118, 300 bus test systems without considering the voltage stability index. In first analysis with considering voltage stability index real power loss minimization, voltage deviation minimization, and voltage stability index enhancement has been attained. In the second evaluation without considered voltage stability index, also power loss reduction achieved. Percentage of power loss reduction is 15.80%, 20.74%, 26.29%, and 14.55% with respect to the base value. Power Loss comparison has been done with other standard methods.