Structures of Urinary Metabolites of Prostaglandin F2alpha in the Rat.

Prostaglandins

“…Metabolites CL (206), CXLIII (202), and CXLVI (199), and CXLV (199) were previously identified in other systems. A similar study on the metabolism of PGF2, in the rat yielded nine metabolites (Table IV) with degradation pathways similar to PGE2 (207,208).…”

Metabolism of prostaglandin E1 in dog kidneys

“…All or at least one of these metabolites are most likely 15-keto PGE, metabolites. Samuelsson (1964), Granstrom, Inger & Samuelsson (1965), Green (1969) and Hamberg (1968) demonstrated that prostaglandins were also metabolized into dinor-and tetranor-prostaglandins by ,B-oxidation in the rat liver mitochondria. Although no study has been made on the /-oxidation of prostaglandins in kidneys, it is reasonable to assume that this would take place.…”

Metabolism of prostaglandin E2 in the rat

“…The O-methyloxime, acetyl, and trimethylsilyl ether derivatives, deuterated (D3MO, D3Ac, D9TMSi) or not, were prepared as described earlier (Gréen, 1969b). Derivatives labeled with three deuterium atoms in the methyl ester group were obtained through transmethylation in 0.5 n NaOCD.-, (Gréen, 1969b).…”

“…The O-methyloxime, acetyl, and trimethylsilyl ether derivatives, deuterated (D3MO, D3Ac, D9TMSi) or not, were prepared as described earlier (Gréen, 1969b). Derivatives labeled with three deuterium atoms in the methyl ester group were obtained through transmethylation in 0.5 n NaOCD.-, (Gréen, 1969b). When a ketone reacts with methoxyamine, the syn and anti isomers of the O-methyloxime are usually formed, and if the keto group is attached to the five-membered ring of a prostaglandin, the isomers separate on a 1 % Se-30 column.…”

“…Recently the structures of one metabolite of PGF2a in human urine (Granstróm and Samuelsson, 1969a) and one in guinea pig urine (Granstróm and Samuelsson, 1969b) have been determined. The identification of five metabolites of PGF2a in rat urine has been published in preliminary form (Gréen, 1969a). The present work, which is concerned with the metabolism of PGE2 in the rat, has led to the identification of nine metabolites, four of which have not been previously identified.…”