Metabolism of prostaglandin E2 in the rat

Determination of prostaglandin synthetase activity in rectal biopsy material and its significance in colonic disease.

“…Measurements of circulating levels of PGs in peripheral blood are of questionable value, as the site of production is not indicated and because some PGs are deactivated in various vascular beds (Piper et al, 1970;Green, 1971). Increased PGs extracted from blood may be due to formation during handling or processing, or due to a reduced breakdown.…”

Prostaglandins

“…to man, the main urinary metabolite was identified as 7a-hydroxy-5,1 1-diketotetranorprosta-1 ,-16-dioic acid (CL) (206). Compound CL also resulted from the administration of tritium-labeled PGE1, 1 I a-hydroxy-9,15-diketoprosta-5,13-dienoic acid, 11 a-hydroxy-9,15-diketoprost-5enoic acid, and 1 1 a-hydroxy-9,I5-diketoprostanoic acid (212) and was previously identified as a urinary metabolite in the rat (204). The sequence of 8oxidation and w-oxidation was not known (212).…”

Metabolism of prostaglandin E analogs in guinea pig and rat liver microsomes

“…In this study, tetranor-v-methylene-PGfi, was utilized to compare co-oxidation in washed microsomes and 20.000 x G supernatant from guinea pig and rat liver because tetranor metabolites usually constitute a large portion of ro-oxidized metabolites appearing in urine from humans and different animals (11)(12)(13)(14)(15)(16)(17)(18).…”