Structures of the O1B and O1C lipopolysaccharide antigens of Escherichia coli

Abstract

The 0-specific moieties of the O1B antigen (lipopolysaccharide) from Escherichia coli O1B:K1 and the O1C antigen from E. coli O1C:K-both consist of L-rhamnose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-mannosamine in a molar ratio of 2:1:1:1. By using fragmentation procedures, methylation analysis, and oneand two-dimensional nuclear magnetic resonance spectroscopy, the structures of these polysaccharides were found to beIn the O1B polysaccharide X is 2, and in the O1C polysaccharide X is 3. With the recently published structure of the OlA polysaccharides (B. Jann, A. S. Shashkov, D. S. Gupta, S. M. Panasenko, and K. Jana, Carbohydr. Polym. 18:51-57 1992), three related 01 antigens are now known. Their common (01-specific) epitope is suggested to be the side-chain N-acetyl-D-mannosamine residue.Escherichia coli strains that express the 01 antigen (lipopolysaccharide [LPS]) and the Kl antigen (capsular polysaccharide) are known to cause extraintestinal infections in humans (20,23,25,29,30). An analysis of extraintestinal E. coli (1, 17, 23) on the basis of sodium dodecyl sulfateMost of the strains were of the 01:K1 serotype and expressed LPSs with two related SDS-PAGE patterns, which were termed O1A and OlAl LPSs. Three strains expressed an LPS with a different SDS-PAGE pattern, which was termed 01B. Another strain had yet another LPS, termed

Role of Avian Pathogenic Escherichia coli Virulence Factors in Bacterial Interaction with Chicken Heterophils and Macrophages

“…Similarly, Rosenberd-Arska et al (52) had noted that a human O78:K80 strain was poorly associated with phagocytes when treated with nonimmune serum and required specific antibodies for effective opsonization. In the present study, we showed that deletion of the rfb locus encoding O78 antigen or replacement of the O78 antigen in strain 7122 by antigens of other O serotypes (O1, O26, and O111) allowed bacteria to associate more extensively with phagocytic cells, particularly when bac- (24,25,29,34). Tsh, pAPEC-1, and curli mutant derivatives of APEC strain 7122 (Table 1) were also only weakly associated with phagocytes, denoting the low importance of Tsh and curli in bacterial association with phagocytic cells for 7122.…”

“…Peritoneal macrophages were infected with opsonized or nonopsonized bacteria at a ratio of 1:10 and incubated at 40°C in a 5% CO 2 -humidified air atmosphere for 1 h. The cells were washed three times with HBSS and incubated for another 2 h in fresh complete RPMI containing 200 g of gentamicin per ml. At this time (T 0 ) and at different incubation times (3,6,12,24, and 48 h) cells were washed three times with HBSS and lysed with 0.1% Triton X-100 in PBS for 5 min at room temperature. Bacteria were serially diluted and enumerated by colony count on TSA plates.…”

Structural diversity inSalmonellaO antigens and its genetic basis

“…Some structures elucidated by us only recently have not been reported earlier and are presented here for the first time. They were established using one-and two-dimensional 1 H-and 13 C-NMR spectroscopy essentially as described (Duus et al, 2000).Three new Salmonella structures, those of O42, O52, and O65 antigens, are identical to the known structures of E. coli O1B (Gupta et al, 1992), O153 (Ratnayake et al, 1994), and O78 (Jansson et al, 1987), respectively.…”

A review of acquired thermotolerance, heat-shock proteins, and molecular chaperones in archaea

“…Daniels and coworkers [57] reported that unpublished Southern analyses indicated a gene with homology to the bacterial HSP70 may be present in H. volcanii. An HSP70 gene was later cloned and sequenced from Halobacterium marismortui [7], suggesting that HSP70 may be among the pulse-labeled protein bands seen in other halophiles. Evidence that the HSP70 gene is heat inducible and sequence information from other HSPs in halophiles remain to be determined.…”

“…The HSPs generally fall into five groups on the basis of their molecular masses: the 'small HSPs' (less than 40 kDa); the HSP60s (60 + 5 kDa); the HSP70s (73 + 5 kDa); the HSP90s (approximately 90 kDa); and the HSP100s (105 + 5 kDa) [6]. Although the classification by molecular mass is somewhat arbitrary, in some cases, particularly the HSP60s and HSP70s, it reflects a clear evolutionary relationship [7,8].…”