Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QTL that colocalized with defense response genes were accumulated by marker-aided selection to develop blast-resistant varieties. One advanced backcross line carrying the major-effect QTL on chromosome (chr) 8, which included a cluster of 12 germin-like protein (OsGLP) gene members, exhibited resistance to rice (Oryza sativa) blast disease over 14 cropping seasons. To determine if OsGLP members contribute to resistance and if the resistance was broad spectrum, a highly conserved portion of the OsGLP coding region was used as an RNA interference trigger to silence a few to all expressed chr 8 OsGLP family members. Challenge with two different fungal pathogens (causal agents of rice blast and sheath blight diseases) revealed that as more chr 8 OsGLP genes were suppressed, disease susceptibility of the plants increased. Of the 12 chr 8 OsGLPs, one clustered subfamily (OsGER4) contributed most to resistance. The similarities of sequence, gene organization, and roles in disease resistance of GLP family members in rice and other cereals, including barley (Hordeum vulgare) and wheat (Triticum aestivum), suggest that resistance contributed by the chr 8 OsGLP is a broad-spectrum, basal mechanism conserved among the Gramineae. Natural selection may have preserved a whole gene family to provide a stepwise, flexible defense response to pathogen invasion.

This review covers the structures and genetics of the 46 O antigens of Salmonella, a major pathogen of humans and domestic animals. The variation in structures underpins the serological specificity of the 46 recognized serogroups. The O antigen is important for the full function and virulence of many bacteria, and the considerable diversity of O antigens can confer selective advantage. Salmonella O antigens can be divided into two major groups: those which have N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) and those which have galactose (Gal) as the first sugar in the O unit. In recent years, we have determined 21 chemical structures and sequenced 28 gene clusters for GlcNAc-/GalNAc-initiated O antigens, thus completing the structure and DNA sequence data for the 46 Salmonella O antigens. The structures and gene clusters of the GlcNAc-/GalNAc-initiated O antigens were found to be highly diverse, and 24 of them were found to be identical or closely related to Escherichia coli O antigens. Sequence comparisons indicate that all or most of the shared gene clusters were probably present in the common ancestor, although alternative explanations are also possible. In contrast, the better-known eight Gal-initiated O antigens are closely related both in structures and gene cluster sequences.

Summary We describe a genetic variation map for the chicken genome containing 2.8 million single nucleotide polymorphisms (SNPs), based on a comparison of the sequences of 3 domestic chickens (broiler, layer, Silkie) to their wild ancestor Red Jungle Fowl (RJF). Subsequent experiments indicate that at least 90% are true SNPs, and at least 70% are common SNPs that segregate in many domestic breeds. Mean nucleotide diversity is about 5 SNP/kb for almost every possible comparison between RJF and domestic lines, between two different domestic lines, and within domestic lines - contrary to the idea that domestic animals are highly inbred relative to their wild ancestors. In fact, most of the SNPs originated prior to domestication, and there is little to no evidence of selective sweeps for adaptive alleles on length scales of greater than 100 kb.

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Although quantitative trait loci (QTL) underpin many desirable agronomic traits, their incorporation into crop plants through marker-assisted selection is limited by the low predictive value of markers on phenotypic performance. Here we used candidate defense response (DR) genes to dissect quantitative resistance in rice using recombinant inbred (RI) and advanced backcross (BC) populations derived from a blast-resistant cultivar, Sanhuangzhan 2 (SHZ-2). Based on DNA profiles of DR genes, RI lines were clustered into two groups corresponding to level of resistance. Five DR genes, encoding putative oxalate oxidase, dehydrin, PR-1, chitinase, and 14-3-3 protein, accounted for 30.0, 23.0, 15.8, 6.7, and 5.5% of diseased leaf area (DLA) variation, respectively. Together, they accounted for 60.3% of the DLA variation and co-localized with resistance QTL identified by interval mapping. Average phenotypic contributions of oxalate oxidase, dehydrin, PR-1, chitinase, and 14-3-3 protein in BC lines were 26.1, 19.0, 18.0, 11.5, and 10.6%, respectively, across environments. Advanced BC lines with four to five effective DR genes showed enhanced resistance under high disease pressure in field tests. Our results demonstrate that the use of natural variation in a few candidate genes can solve a long-standing problem in rice production and has the potential to address other problems involving complex traits.

MG-132, a proteasome inhibitor, can upregulate nuclear factor (NF) erythroid 2-related factor 2 (Nrf2)-mediated antioxidative function and downregulate NF-κB-mediated inflammation. The present study investigated whether through the above two mechanisms MG-132 could provide a therapeutic effect on diabetic cardiomyopathy in the OVE26 type 1 diabetic mouse model. OVE26 mice develop hyperglycemia at 2-3 wk after birth and exhibit albuminuria and cardiac dysfunction at 3 mo of age. Therefore, 3-mo-old OVE26 diabetic and age-matched control mice were intraperitoneally treated with MG-132 at 10 μg/kg daily for 3 mo. Before and after MG-132 treatment, cardiac function was measured by echocardiography, and cardiac tissues were then subjected to pathological and biochemical examination. Diabetic mice showed significant cardiac dysfunction, including increased left ventricular systolic diameter and wall thickness and decreased left ventricular ejection fraction with an increase of the heart weight-to-tibia length ratio. Diabetic hearts exhibited structural derangement and remodeling (fibrosis and hypertrophy). In diabetic mice, there was also increased systemic and cardiac oxidative damage and inflammation. All of these pathogenic changes were reversed by MG-132 treatment. MG-132 treatment significantly increased the cardiac expression of Nrf2 and its downstream antioxidant genes with a significant increase of total antioxidant capacity and also significantly decreased the expression of IκB and the nuclear accumulation and DNA-binding activity of NF-κB in the heart. These results suggest that MG-132 has a therapeutic effect on diabetic cardiomyopathy in OVE26 diabetic mice, possibly through the upregulation of Nrf2-dependent antioxidative function and downregulation of NF-κB-mediated inflammation.

Rh-based catalysts might resolve the longstanding problem of poor C1 pathway efficiency toward an ethanol oxidation reaction (EOR). To facilitate the rational design and preparation of Rh-based EOR catalysts, here we fundamentally study ethanol adsorptive dissociation and oxidation on an Rh electrode surface by electrochemical infrared absorption spectroscopy. First, real-time infrared spectral results show that ethanol could be easily split on an Rh surface into CO ad and CH x intermediates only in alkaline media but not in acidic media. Second, the onset oxidation potential of EOR on Rh is ca. 180 mV more negative than that on Pd and Pt electrode in alkaline media. The EOR J f /J b ratio is ca. 5.73, 1.62, and 0.35 on Rh, Pt, and Pd, respectively, suggesting that CO ad and/or CH x intermediates could be readily oxidized into CO 2 on Rh. Accordingly, the apparent selectivity efficiency of the C1 pathway (η) is estimated to be 100% when the potential is at 0.4−0.6 V vs RHE, subsequently η sharply decreases to zero at 0.65−0.8 V vs RHE, and then, η gradually rebounds to ca. 15% when the potential moves positively. This work may provide some theoretical support for fabricating highly efficient EOR catalysts.

To investigate the possible mechanism(s) by which dietary manganese (Mn) levels and sources modulate the expression of the manganese-containing superoxide dismutase (MnSOD) gene at both the transcriptional and translational levels, we used 432 8-d-old male broiler chicks in a 1 plus 4 × 2 design. Chickens were given either a diet without Mn supplementation [control (C)] or diets supplemented with 100 (optimal) or 200 (high) mg Mn/kg diet from inorganic Mn sulfate (I) or 3 organic complexes of Mn and amino acids with weak (W), moderate (M), or strong (S) chelation strength up to 21 d of age. Compared with C chicks, chicks fed Mn-supplemented diets had higher (P < 0.01) Mn concentrations, specificity protein 1 (Sp1) DNA-binding activities, MnSOD mRNA levels, MnSOD mRNA-binding protein (MnSOD-BP) RNA-binding activities, MnSOD protein concentrations, and MnSOD activities within heart tissue, but lower (P < 0.01) heart activating protein-2 (AP-2) DNA-binding activities. Chicks fed M diets had higher (P < 0.05) heart Mn concentrations, MnSOD mRNA levels, and MnSOD-BP RNA-binding activities compared with those fed the I and W diets and lower (P < 0.01) AP-2 DNA-binding activities than those fed other treatment diets. These results suggest that dietary Mn could modulate the expression of the MnSOD gene in broilers by altering Sp1 and AP-2 DNA-binding activities at the transcriptional level and enhancing MnSOD-BP RNA-binding activity at the translational level. Additionally, an organic Mn source with moderate chelation strength could be more effective than other Mn sources in activating MnSOD gene expression at both the transcriptional and translational levels.