Structures,Locations, and Transfer Frequencies of Genetic Elements ConferringHigh-Level Gentamicin Resistance in
            <i>Enterococcusfaecalis</i>
            Isolates inGreece

Daikos, George L.; Bamias, G; Kattamis, Christos; Zervos, Marcus J.; Chow, Joseph W.; Christakis, G. B.; Petrikkos, George; Triantafyllopoulou, P.; Alexandrou, H.; Syriopoulou, Vassiliki

doi:10.1128/aac.47.12.3950-3953.2003

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…By contrast, the Tn4001-related element is able to render transposition [15]. However, the findings of Daikos et al [16] indicate that the non-truncated elements are more stable, whereas the truncated variants are mobile and transfer GM resistance more efficiently.…”

Section: Resultsmentioning

confidence: 85%

A novel structure of Tn4001-truncated element, type V, in clinical enterococcal isolates and multiplex PCR for detecting aminoglycoside resistance genes

Leelaporn

Yodkamol

Waywa

et al. 2008

International Journal of Antimicrobial Agents

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Section: Resultsmentioning

confidence: 85%

A novel structure of Tn4001-truncated element, type V, in clinical enterococcal isolates and multiplex PCR for detecting aminoglycoside resistance genes

Leelaporn

Yodkamol

Waywa

et al. 2008

International Journal of Antimicrobial Agents

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“…It is becoming more and more common to find many bacteria developing resistance to most common antibiotics. Enterococci exhibiting HLGR have been reported as an important cause of hospital acquired infection from many geographical locations, including European countries and the United States [ 13 , 14 ]. Gentamicin together with a cell wall active agent is one of the common antibiotics used against serious enterococcal infections [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Basic research In vitro antibacterial activity of seven Indian spices against high level gentamicin resistant strains of enterococci

Sharma

Chapagain

Chitra

et al. 2015

aoms

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IntroductionThe aim of the study was to explore the in vitro antibacterial activity of seven ethanolic extracts of spices against high level gentamicin resistant (HLGR) enterococci isolated from human clinical samples.Material and methodsTwo hundred and fifteen enterococcal strains were isolated from clinical samples. High level gentamicin resistance in ethanolic extracts of cumin (Cuminum cyminum), cinnamon (Cinnamomum zeylanicum), ginger (Zingiber officinale), fenugreek (Trigonella foenum-graecum), cloves (Syzygium aromaticum), cardamom (Elettaria cardamomum Maton) and black pepper (Piper nigrum) were prepared using Soxhlet apparatus. The antibacterial effect of the extracts was studied using the well diffusion method. Statistical analysis was carried out by χ2 test using SPSS 17 software.ResultsOnly cinnamon and ginger were found to have activity against all the isolates, whereas cumin and cloves had a variable effect on the strains. Fenugreek, black pepper and cardamom did not show any effect on the isolates. The zone diameter of inhibition obtained for cinnamon, ginger, cloves and cumin was in the range 31–34 mm, 27–30 mm, 25–26 mm and 19–20 mm respectively.ConclusionsCinnamomum zeylanicum and Z. officinale showed the maximum antibacterial activity against the enterococcal isolates followed by S. aromaticum and C. cyminum. The findings of the study show that spices used in the study can contribute to the development of potential antimicrobial agents for inclusion in the anti-enterococcal treatment regimen.

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“…In parallel, none or low resistant rates against synercid or linezolid were seen among our isolates because these antimicrobial agents have recently been introduced into the market for the treatment of Gram‐positive bacterial infections in Iran. Of all gentamicin resistant‐associated genes, the aac ( 6 ′) ‐Ie‐aph ( 2 ″) ‐Ia gene is considered as the most important and widely reported gene involved in the human HLGR enterococcal isolates (Daikos et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

“…HLGR could, however, eliminate the synergistic effect penicillin and an aminoglycoside (Daikos et al. 2003).…”

Section: Introductionmentioning

confidence: 99%

Clonal groups of high-level gentamicin-resistantEnterococcus faeciumisolated from municipal wastewater and clinical samples in Tehran, Iran

Saifi

Pourshafie

Dallal

et al. 2009

Letters in Applied Microbiology

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Aims: Clonality among high‐level gentamicin‐resistant Enterococcus faecium (HLGR‐EF) isolates obtained from clinical and sewage treatment plants (STP) were investigated using PhePlate system (PhP), ribotyping and pulsed‐field gel electrophoresis (PFGE). Methods and Results: During 1 year study (September 2005–2006), a total of 106 HLGR‐EF isolates were collected from clinical (n = 48) and STP (n = 58) samples in Tehran, Iran. Biochemical fingerprinting of these isolates using the PhP showed the presence of 21 PhP types (diversity index, Di = 0·97) among the clinical and 21 PhP types (Di = 0·91) among the STP isolates. Representative isolates of each PhP type (n = 42) were further characterized by the ribotyping method. Sixteen ribotypes were identified among the isolates with five types shared between the clinical and STP isolates. PFGE recognized 24 clonal types among these isolates with three pulsotypes shared between the clinical and STP isolates. Combination of the two techniques (PFGE and ribotyping) resulted in 24 (Di = 0·96) and 16 (Di = 0·93) types among the strains isolated from clinical and STP samples, respectively. Conclusions: We concluded that the combination of PhP typing, ribotyping and PFGE could be extremely discriminatory when examining HLGR‐EF isolates. Significance and Impact of the Study: The emergence of highly diverse HLGR‐EF population in Iran is of serious concern especially because of their multi‐resistances.

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Structures,Locations, and Transfer Frequencies of Genetic Elements ConferringHigh-Level Gentamicin Resistance in Enterococcusfaecalis Isolates inGreece

Cited by 12 publications

References 22 publications

A novel structure of Tn4001-truncated element, type V, in clinical enterococcal isolates and multiplex PCR for detecting aminoglycoside resistance genes

A novel structure of Tn4001-truncated element, type V, in clinical enterococcal isolates and multiplex PCR for detecting aminoglycoside resistance genes

Basic research In vitro antibacterial activity of seven Indian spices against high level gentamicin resistant strains of enterococci

Clonal groups of high-level gentamicin-resistantEnterococcus faeciumisolated from municipal wastewater and clinical samples in Tehran, Iran

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