Structures and Antibacterial Properties of PLA-based Ciprofloxacin Composite Films Deposited by Low-Electron Beam Dispersion

Abstract

Polylactic acid (PLA)-based ciprofloxacin (Cip) antibacterial films with mass ratio PLA:Cip=1:1 were prepared by low-electron beam dispersion (EBD). The molecular structure, chemical composition and morphology of PLA-based ciprofloxacin antibacterial films were investigated by XPS, FTIR, liquid NMR and SEM. The antibacterial activity of composite films was tested against E. coli ATCC 25922 and S. aureus ATCC 12600 using the agar diffusion method on the solid LB agar medium. XPS and FTIR analysis showed the presence of an antibacterial ingredient in the composite films. Using NMR, it has been shown that the molecular structure of a monolayer of ciprofloxacin is fully consistent with the molecular structure of the initial ciprofloxacin powder. High antibacterial activity of the composite films has been also established and the layers still possess antibacterial activity with regard to S. aureus even after 7 days of leaching in an isotonic solution. The thermal treatment indicates that the composite films can withstand temperatures of 180 °C and keep its structure unchanged.

Deposition of Copper on Poly(Lactide) Non-Woven Fabrics by Magnetron Sputtering—Fabrication of New Multi-Functional, Antimicrobial Composite Materials

“…Substantial importance in this field is played by PLA composites equipped with antibacterial additivities, in the majority organic bactericides (e.g., ampicillin [ 9 ] chlorhexidine [ 10 , 11 ], ciprofloxacin [ 12 , 13 ], doxycyclin [ 14 ], gentamycin [ 15 ], oflaxin and vancomycin [ 16 ], triclosan [ 17 , 18 , 19 ], and/or curcumin [ 20 ], etc.). Due to an established role of metal nanoparticles in medicine [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ], the group of increasing importance is PLA-inorganic hybrids [ 34 ], due to their high antibacterial efficiency, simplicity and also low cost of technological preparation.…”

PCL Based CIP-Loaded Double-Layer Films Deposited by Low-Electron Beam Dispersion Method and its Antibacterial Properties

“…It confirms that PCL and CIP are integrated and are relatively Eurasian Chemico-Technological Journal 22 (2020) 255-262 tightly and uniformly mechanically mixed together. These results indicate that the CIP powder did not degrade significantly under the activation of low-electron beams, which is attributed to the fact that the tendency of ciprofloxacin evaporation is accessible without strong heating and radiation exposure [11].…”

“…As it was shown that the antibacterial activity of CIP was tested against E. coli ATCC 25922 and S. aureus ATCC 12600 using the agar diffusion method on the solid LB agar medium [11]. It had proved that CIP has stronger antibacterial activity against S. aureus than E. coli, so in this article, we choose S. aureus to test.…”

“…Nowadays, the most widely used preparation methods are mainly divided into physical vapor deposition (PVD) and chemical vapor deposition (CVD). EBD is a PVD method carrying out rapid deposition and simple operation and the dispersion of electron beam is beneficial to obtain uniform high-purity and high-density films [11]. Vacuum evaporation is conducive to avoid the contamination and oxidation of the target materials and form an antibacterial layer which can prolong the drug release time [12].…”