Structured Triacylglycerol Containing Medium-Chain Fatty Acids in sn-1(3) Facilitates the Absorption of Dietary Long-Chain Fatty Acids in Rats

Carvajal, Octavio; Sakono, Masafumi; Sonoki, Hirofumi; Nakayama, Masahiro; Kishi, Taiji; Sato, Masao; Ikeda, Ikuo; Sugano, Michihiro; Imaizumi, Kazunori

doi:10.1271/bbb.64.793

Cited by 20 publications

(11 citation statements)

References 24 publications

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“…13 C-Labeled 18:2n-6 may have stayed in the intestinal lumen, but it seems unlikely that the pancreatic lipase would not have hydrolyzed the oil bolus, and the flow of saline-glucose solution (2 mL/h) through the gastrostomy tube also secured the gastric emptying. However, during a feeding study of 4 wk, approximately 15% of the dietary structured TAG were detected in feces (41).…”

Section: Discussionmentioning

confidence: 97%

Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG

Vistisen

Høy

2003

Lipids

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The potential of the specific structured TAG MLM [where M = caprylic acid (8:0) and L = linoleic acid (18:2n-6)] is the simultaneous delivery of energy and EFA. Compared with long-chain TAG (LLL), they may be more rapidly hydrolyzed and absorbed. This study examined the lymphatic recoveries of intragastrically administered L*L*L*, M*M*M*, ML*M, and ML*L* (where * = 13C-labeled FA) in rats. Lymph lipids were separated into lipid classes and analyzed by GC combustion isotope ratio MS. The recoveries of lymph TAG 18:2n-6 8 h after administration of L*L*L*, ML*M, and ML*L* were 38.6, 48.4, and 49.1%, respectively, whereas after 24 h the recoveries were approximately 50% in all experimental groups. The exogenous contribution to lymph TAG 18:2n-6 was approximately 80 and 60% at maximum absorption of the specific structured TAG and L*L*L*, respectively, 3-6 h after administration. The tendency toward more rapid recovery of exogenous long-chain FA following administration of specific structured TAG compared with long-chain TAG was probably due to fast hydrolysis. The lymphatic recovery of 8:0 was 2.2% 24 h after administration of M*M*M*. This minor lymphatic recovery of exogenous 8:0 was probably due to low stimulation of chylomicron formation. These results demonstrate tendencies toward faster lymphatic recovery of long-chain FA after administration of specific structured TAG compared with long-chain TAG.

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Section: Discussionmentioning

confidence: 97%

Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG

Vistisen

Høy

2003

Lipids

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“…The following variables were optimized: water/ethanol ratio (the best results were attained with a water/ethanol ratio of 30:70, w/w), the solvent/FFA-STAG mixture ratio (3:1, w/w) and the number of extraction steps (3)(4)(5). In these conditions highly pure STAGs (93-96%) were obtained with a yield of 85%.…”

Section: Introductionmentioning

confidence: 99%

“…This composition and structure is interesting because absorption of the fatty acids depends on their type and on their position in the TAG molecule. Thus, pancreatic lipase, which is 1,3-specific, hydrolyzes the TAG of the diet giving free fatty acids and 2-monoacylglycerol (2-MAG) [4]; in addition this lipase shows higher activity toward medium-chain fatty acids than toward long-chain ones, especially PUFAs [5,6]. The liberated medium-chain free fatty acids are directly absorbed into the portal vein and used to provide energy [7,8], while the 2-MAG (with the essential long-chain fatty acid) are well absorbed via the lymphatic route [4] and are often used in biosynthesis [7,9].…”

Section: Introductionmentioning

confidence: 99%

Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor

Hita

Robles

Camacho

et al. 2009

Biochemical Engineering Journal

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“…Likewise, Jensen et al (12) observed a higher mesenteric lymphatic transport of linoleic acid (18:2n-6) after administration of MLM compared with a randomized oil (the same fatty acid composition as MLM, but no specific structure at the glycerol backbone) in rats fed 20 wt% fish oil compared with rats fed 20 wt% vegetable oil. Furthermore, the fecal excretion of fat was lower in rats fed structured triacylglycerol MLM compared with a randomized oil for 3 wk (13). These studies indicate that alterations in fat absorption could be caused by variation in the dietary fatty acid composition for several days or weeks.…”

mentioning

confidence: 69%

Lymphatic recovery of exogenous oleic acid in rats on long chain or specific structured triacylglycerol diets

Vistisen

Høy

2006

Lipids

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Specific structured triacylglycerols, MLM (M = medium-chain fatty acid, L = long-chain fatty acid), rapidly deliver energy and long-chain fatty acids to the body and are used for longer periods in human enteral feeding. In the present study rats were fed diets of 10 wt% MLM or LLL (L = oleic acid [18:1 n-9], M = caprylic acid [8:01) for 2 wk. Then lymph was collected 24 h following administration of a single bolus of 13C-labeled MLM or LLL. The total lymphatic recovery of exogenous 18:1 n-9 24 h after administration of a single bolus of MLM or LLL was similar in rats on the LLL diet (43% and 45%, respectively). However, the recovery of exogenous 18:1 n-9 was higher after a single bolus of MLM compared with a bolus of LLL in rats on the MLM diet (40% and 24%, respectively, P = 0.009). The recovery of lymphatic 18:1 n-9 of the LLL bolus tended to depend on the diet triacylglycerol structure and composition (P= 0.07). This study demonstrated that with a diet containing specific structured triacylglycerol, the lymphatic recovery of 18:1 n-9 after a single bolus of fat was dependent on the triacylglycerol structure of the bolus. This indicates that the lymphatic recovery of long-chain fatty acids from a single meal depends on the overall long-chain fatty acid composition of the habitual diet. This could have implications for enteral feeding for longer periods.

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Structured Triacylglycerol Containing Medium-Chain Fatty Acids in sn-1(3) Facilitates the Absorption of Dietary Long-Chain Fatty Acids in Rats

Cited by 20 publications

References 24 publications

Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG

Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG

Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor

Lymphatic recovery of exogenous oleic acid in rats on long chain or specific structured triacylglycerol diets

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Structured Triacylglycerol Containing Medium-Chain Fatty Acids in sn-1(3) Facilitates the Absorption of Dietary Long-Chain Fatty Acids in Rats

Cited by 20 publications

References 24 publications

Recoveries of rat lymph FA after administration of specific structured13C‐TAG

Recoveries of rat lymph FA after administration of specific structured13C‐TAG

Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor

Lymphatic recovery of exogenous oleic acid in rats on long chain or specific structured triacylglycerol diets

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Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG

Recoveries of rat lymph FA after administration of specific structured¹³C‐TAG