Structured Thin Organic Active Layers and Their Use in Electrochemical Biosensors

Mills, C. A.; Pla-Roca, Mateu; Martin, C.; Lee, Michael; Kuphal, Mathias; Sisquella, Xavier; Martínez, Elena; Errachid, Abdelhamid; Samitier, J.

doi:10.1177/002029400704000307

Cited by 2 publications

(2 citation statements)

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“…One of the rational ways to achieve such functional enzyme organization is to immobilize the proteins in well-dened thin layers. [7][8][9][10] In order to form such a network, in which enzyme molecules not only retain their activities, but are also electroactive, one needs to choose building blocks that are capable of exchanging electrons with the enzyme and do not cause enzyme denaturation. Conjugated polymers such as polypyrroles and polyanilines have already been proven to represent suitable materials for this purpose.…”

Section: Introductionmentioning

confidence: 99%

A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

Sarauli

Xu²,

Dietzel

et al. 2014

J. Mater. Chem. B

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Section: Introductionmentioning

confidence: 99%

A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

Sarauli

Xu²,

Dietzel

et al. 2014

J. Mater. Chem. B

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“…To some extent, enzyme immobilization allows the variation and adaptation of experimental parameters, which is one of the major topics for construction of amperometric biosensors. [6][7][8] For some amperometric biosensors (e.g. assigned for the detection of glucose), extension of the linear range is sought that can be easily reached by an increase of the apparent Michaelis-Menten constant, K M(app) .…”

Section: Introductionmentioning

confidence: 99%

Self-encapsulation of oxidases as a basic approach to tune the upper detection limit of amperometric biosensors

Ramanavičius

Kaušaitė

2008

Analyst

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This study describes a new, basic procedure for the tuning of some analytical parameters of enzymatic biosensors that are based on hydrogen peroxide-producing oxido-reductases. An amperometric biosensor based on glucose oxidase (GOx) (EC 1.1.3.4) from Penicillum vitale, immobilized on a carbon rod electrode by cross-linking with glutaraldehyde, was exploited as a model system for demonstration of the approach described here. Such an important analytical parameter as the upper detection limit was dramatically changed by the formation of a polypyrrole conducting polymer layer by the GOx-induced polymerization of polypyrrole (Ppy). An increase in the upper detection limits for differently modified electrodes was estimated by calculation of the apparent Michaelis-Menten constant [K(M(app))]. A significant increase in the long-term stability of the GOx-based electrode modified by Ppy (GOx/Ppy) was detected compared with that of an unmodified one. Further application of this approach, based on the self-encapsulation of glucose oxidase and other oxidases, is predicted for such biosensors where extension of the detection rate as well as K(M(app)) are required.

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Structured Thin Organic Active Layers and Their Use in Electrochemical Biosensors

Cited by 2 publications

References 50 publications

A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

Self-encapsulation of oxidases as a basic approach to tune the upper detection limit of amperometric biosensors

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