Structured illumination microscopy using a photonic chip

Helle, Øystein Ivar; Dullo, Firehun Tsige; Lahrberg, Marcel; Tinguely, Jean-Claude; Hellesø, Olav Gaute; Ahluwalia, Balpreet Singh

doi:10.1038/s41566-020-0620-2

Cited by 115 publications

(106 citation statements)

References 48 publications

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“…Taken together, Fourier ptychography in combination with enlarged illumination wave vectors can be a promising avenue to enable nanoscopic imaging without the requirement of extrinsic labels as long as the reconstruction procedures can be improved. The more robust ROCS processing presents meanwhile a suitable alternative and could potentially be used in conjunction with fluorescence on-chip superresolution microscopy [ 41 , 42 ]. As such a combination would consist purely of widefield imaging techniques, a considerable increase in throughput is achievable as compared to scanning approaches like nearfield scanning optical microscopy.…”

Section: Discussionmentioning

confidence: 99%

Super-condenser enables labelfree nanoscopy

Ströhl¹,

Opstad²,

Tinguely³

et al. 2019

Opt. Express

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Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines Fourier ptychography with waveguide microscopy to realize a super-condenser featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the employed Si 3 N 4 waveguide material. We produce the required coherent plane wave illumination for Fourier ptychography over imaging areas 400 µm 2 in size via adiabatically tapered single-mode waveguides and tackle the overlap constraints of the Fourier ptychography phase retrieval algorithm two-fold: firstly, the directionality of the illumination wave vector is changed sequentially via a multiplexed input structure of the waveguide chip layout and secondly, the wave vector modulus is shortend via step-wise increases of the illumination light wavelength over the visible spectrum. We validate the method via in silico and in vitro experiments and provide details on the underlying image formation theory as well as the reconstruction algorithm.

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Section: Discussionmentioning

confidence: 99%

Super-condenser enables labelfree nanoscopy

Ströhl¹,

Opstad²,

Tinguely³

et al. 2019

Opt. Express

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“…Helle et al. [ 102 ] reported a cSIM demonstration using Si 3 N 4 waveguide ( n f = 1.7). It reported a 1.2 times resolution enhancement compared with TIRF‐SIM, corresponding to a lateral resolution of 117 nm (NA = 1.2, λ ex = 660 nm).…”

Section: Superresolution Imaging Based On Sfs Methodsmentioning

confidence: 99%

“…To obtain uniform illumination over a large FOV, a single‐mode waveguide is adiabatically tapered [ 210,211 ] to a wide waveguide (theoretically applies for waveguides with over several mm 2 areas). By integrating phase modulation (such as on‐chip thermo‐optical modulation), [ 102 ] cSIM may have opened an avenue for high‐throughput, large‐producible, and miniaturized superresolution imaging.…”

Section: Superresolution Imaging Based On Sfs Methodsmentioning

confidence: 99%

“…Thus, the innovations on illumination method led to many variants based on SIM with higher resolution, such as SSIM, [44] NL-SIM, [46] PSIM, [52,103] LPSIM, [106,107,159,199,200] and cSIM. [102] The theory also applies to any illumination patterns with high spatial frequencies, such as confocal microscopy and the related spot scanning SIM, which exploit a diffraction-limited excitation focus (or multi-foci) to scan the sample. The excitation focus contains all spatial frequencies up to the cutoff frequency of the condenser objective, enabling a doubled resolution.…”

Section: Evanescent Fields Illuminated Sfs Labeled Microscopymentioning

confidence: 99%

“…Generally speaking, superresolution microscopy utilizes mainly two kinds of evanescent waves for illumination, evanescent fields at dielectric waveguide boundaries, [97] and surface plasmon polaritons (SPPs) or localized plasmons (LPs) at metal/dielectric interfaces. [98,99] Several papers have reported evanescent-wave-assisted SFS methods using delicately designed structures, including evanescent fields illuminated SFS label-free microscopy, [51,100] evanescent fields illuminated SFS labeled microscopy, [101,102] plasmonic structured illumination microscopy (PSIM), [52,[103][104][105] and localized plasmonic structured illumination microscopy (LPSIM). [106,107] This review aims to provide a systematic discussion on the evanescent-wave-assisted SFM superresolution microscopy techniques (Figure 1) since other reviews only have covered parts of this promising research area.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Far‐Field Superresolution Imaging via Spatial Frequency Modulation

Tang

Liu

Zhong

et al. 2020

Laser & Photonics Reviews

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The diffraction limit substantially impedes the resolution of the conventional optical microscope. Under traditional illumination, the high-spatial-frequency light corresponding to the subwavelength information of objects is located in the near-field in the form of evanescent waves, and thus not detectable by conventional far-field objectives. Recent advances in nanomaterials and metamaterials provide new approaches to break this limitation by utilizing large-wavevector evanescent waves. Here, a comprehensive review of this emerging and fast-growing field is presented. The current superresolution imaging techniques based on evanescent-wave-assisted spatial frequency modulation, including hyperlens, microsphere lens, and evanescent field-illuminated spatial frequency shift microscopy, are illustrated. They are promising in investigating unobserved details and processes in fields such as medicine, biology, and material research. Some current challenges and future possibilities of these superresolution methods are also discussed.

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Spatial‐frequency‐shift Super‐resolution Imaging Based on Micro/nanomaterials

Tang,

Yang

2023

Optical Imaging and Sensing

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Structured illumination microscopy using a photonic chip

Cited by 115 publications

References 48 publications

Super-condenser enables labelfree nanoscopy

Super-condenser enables labelfree nanoscopy

Far‐Field Superresolution Imaging via Spatial Frequency Modulation

Spatial‐frequency‐shift Super‐resolution Imaging Based on Micro/nanomaterials

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