Structure studies of collocalia mucoid

Kathan, Ralph H.; Weeks, Dianna I.

doi:10.1016/0003-9861(69)90319-1

Cited by 67 publications

(26 citation statements)

References 11 publications

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“…Collocalia niucoid, the nest-cementing glycopratein substance of Chinese swift, which can be obtained [2] by aqueous extraction of edible bird's nest, a Chinese delicacy, is reported [2] to contain about 9% of Neu5Ac and is, therefore, a practical source of Neu5Ac. The isolation of Neu5Ac from this glycoprotein has been the subject of several publications, using hot water extraction [3, 41, acid hydrolysis [5] and combined enzymatic and acid hydrolysis [6].…”

Section: Neu5acmentioning

confidence: 99%

“…The most significant ions in the mass spectra of the peracetylated methyl (2) and trideuteriomethyl ester (3J derivatives of 1, together with relative intensities and tentative assignments, are summarized in Table 2. The corresponding data for the pertrimethylsilylated derivative ( 4J of the methyl ester of 1 and that for the petrimethylsilylated derivative (3 of the catalytically reduced methyl ester of 1 are shown in Table 3.…”

Section: Mass Spectrometry Of the Derivatives Of Lmentioning

confidence: 99%

“…However, a suitable substituent at HO-4 of NeuSAc, e.g. acetyl, could render this group more amenable to departure followed by anhydro ring formation, and indeed, it has been postulated [2] that in Collocolia mucoid the sialic acid is probably N-acetyl-4-0-acetylneuraminic acid. Taking into account the lability of 0-acetyl groups in polysaccharides to even very mild basic conditions, it is not surprising that this finding could not be confirmed by others [6], nor is it contradictory that a recent study [22] on the structure of the carbohydrate portion of Colloculia mucoid did not reveal the presence of an 0-acetyl group since, if present, it would have been removed under the degradative conditions reported [22].…”

Section: Acnh-ch-ch-ome Simentioning

confidence: 99%

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4,8-Anhydro-N-acetylneuraminic acid. Isolation from edible bird's nest and structure determination

1987

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A new, sialic-acid-derived compound was isolated from the acid hydrolysate of edible bird's nest by ionexchange chromatography. Combined use of mass spectroscopy and 'H-and 13C-NMR spectroscopy established that it is the 4,g-anhydro derivative of N-acetylneuraminic acid and that in solutions it exists in two tautomeric forms.The formation of the new compound supports and earlier finding that in the glycoprotein of edible bird's nest at least a portion of N-acetylneuraminic acid is acetylated at HO-4.Recently we needed gram quantities of N-acetyl-D-neuraminic acid (Neu5Ac) for synthetic work aimed at reconstructing Neu5Ac-containing oligosaccharides of the capsular polysaccharides of group B streptococci [l]. Neu5AcCollocalia niucoid, the nest-cementing glycopratein substance of Chinese swift, which can be obtained [2] by aqueous extraction of edible bird's nest, a Chinese delicacy, is reported [2] to contain about 9% of Neu5Ac and is, therefore, a practical source of Neu5Ac. The isolation of Neu5Ac from this glycoprotein has been the subject of several publications, using hot water extraction [3, 41, acid hydrolysis [5] and combined enzymatic and acid hydrolysis [6]. Although in our hands hot water extraction of edible bird's nest gave no practical amounts of NeuSAc, mild acid hydrolysis, followed by ionexchange chromatographic isolation, as described [5], gave Neu5Ac in yields comparable to those published [3 -51. Also obtained was a second, previously uncharacterized compound (I). In the present paper we describe the isolation and structural investigation of this new compound. MATERIALS AND METHODSEdible bird's nest was obtained from a local Chinese grocery. N-Acetyh-neuraminic acid was obtained from Pfanstiehl Laboratories, Inc. (Waukegan, IL) and was used without purification. All other chemicals were analytical grade commercial reagents. Analytical methodsThin-layer chromatography was carried out on precoated, 0.25-mm silica gel plates (Kieselgel 60 F254 of E. Merck, Darmstadt, FRG; cat. no. 5765) with propanol/water ( 3 : 1, by vol.) (solvent A) and on pre-coated, 0.1-mm cellulose plates (pre-coated TLC Sheets, cellulose of E. Merck, Darmstadt, FRG; cat. no. 5537) with propanol/butanol/O.l M HCI (2:1:1, by vol.) [6] (solvent B). Detection was made by spraying with resorcinol/Cu2 + reagent [7] followed by heating at 110°C.Colorimetric tests, using resorcinol/Cu2 +, periodic acid/ resorcinol/Cu2 + and periodic acidlthiobarbituric acid were carried out as published [7 -91 using an LKB Ultrospec 4050 spectrophotometer.Optical rotation was measured with a Perkin-Elmer 243 automatic polarimeter.Combined gas-liquid chromatography/mass spectrometry of derivatives of lwas performed on a Hewlett-Packard 5985B instrument operated in the electron impact mode at an ionization potential of 70 eV, ion-source temperature 200 "C, using a 3% OV-17-coated fused-silica capillary column (25 m x 0.3 mm) at a temperature program at 200 -260 "C at 2 "C/ min .The 'H-and 13C-NMR spectra were recorded with a Bruker AM-500 spectromet...

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Section: Neu5acmentioning

confidence: 99%

Section: Mass Spectrometry Of the Derivatives Of Lmentioning

confidence: 99%

Section: Acnh-ch-ch-ome Simentioning

confidence: 99%

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4,8-Anhydro-N-acetylneuraminic acid. Isolation from edible bird's nest and structure determination

1987

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“…According to [4], the order of composition (form lowest to highest) in bird's nest is; lipid (0.14 -1.28%), ash (2.1%), carbohydrate (25.62 -27.76%) and protein (62 -63%). The major nutrient components of EBN are carbohydrates and glycoproteins [5]. Glycoprotein is of great importance in all higher organisms, vertebrate and invertebrate, that the body cavities of the respiratory, digestive and urogenital tracts are lined by a cell layer secreting viscous glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

“…Glycoprotein is of great importance in all higher organisms, vertebrate and invertebrate, that the body cavities of the respiratory, digestive and urogenital tracts are lined by a cell layer secreting viscous glycoproteins. The glycoprotein in bird's nest contains about 9% sialic acid, 4.19 -7.2% galactosamine, about 5.3% glucosamine, 5.03 -16.9% galactose, and about 0.7% fucose [5,6]. The most abundant amino acids are serine, threonine, aspartic acid, glutamic acid, proline, and valine [5].…”

Section: Introductionmentioning

confidence: 99%

Effect of enzymatic hydrolysis of pancreatin and alcalase enzyme on some properties of edible bird’s nest hydrolysate

Khushairay¹,

Ayub²,

Babji³

2014

AIP Conference Proceedings

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Abstract. Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of several different swiftlet species. It is widely consumed as a health food due to its high beneficial effects to human health and has been considered to be one of the most precious food items by the Chinese for thousands of years. The aim of this study was to evaluate the effect of enzymatic hydrolysis on protein solubility (μg/g), the degree of hydrolysis (DH%), and peptide content (μg/g) of edible bird's nest hydrolysate. Initial protein solubility of boiled EBN was 25.5mg/g EBN. Treating the solubilized EBN with pancreatin 4NF for 1.0 -1.5hours increased EBN protein solubility to 163.9mg/g and produced hydrolysate with DH% of 86.5% and 109.5mg/g peptide. EBN hydrolyzed with alcalase for 1.5 hours produced hydrolysate with protein solubility of 86.7mg/g, 82.7 DH% and had 104.1mg/g peptide content.

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Molecular sex identification of Malaysian White‐Nest Swiftlet (Aerodramus fuciphagus Thunberg, 1812)

Osman

Sugnaseelan

Panandam³

et al. 2020

Ecology and Evolution

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Structure studies of collocalia mucoid

Cited by 67 publications

References 11 publications

4,8-Anhydro-N-acetylneuraminic acid. Isolation from edible bird's nest and structure determination

4,8-Anhydro-N-acetylneuraminic acid. Isolation from edible bird's nest and structure determination

Effect of enzymatic hydrolysis of pancreatin and alcalase enzyme on some properties of edible bird’s nest hydrolysate

Molecular sex identification of Malaysian White‐Nest Swiftlet (Aerodramus fuciphagus Thunberg, 1812)

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