Effect of enzymatic hydrolysis of pancreatin and alcalase enzyme on some properties of edible bird’s nest hydrolysate

Abstract

Abstract. Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of several different swiftlet species. It is widely consumed as a health food due to its high beneficial effects to human health and has been considered to be one of the most precious food items by the Chinese for thousands of years. The aim of this study was to evaluate the effect of enzymatic hydrolysis on protein solubility (μg/g), the degree of hydrolysis (DH%), and peptide content (μg/g) of edible bird's nest hydrolysate. Initial protein solubility of boiled EBN was 25.5mg/g EBN. Treating the solubilized EBN with pancreatin 4NF for 1.0 -1.5hours increased EBN protein solubility to 163.9mg/g and produced hydrolysate with DH% of 86.5% and 109.5mg/g peptide. EBN hydrolyzed with alcalase for 1.5 hours produced hydrolysate with protein solubility of 86.7mg/g, 82.7 DH% and had 104.1mg/g peptide content.

Recovery of value‐added glycopeptides from edible bird's nest (EBN) co‐products: enzymatic hydrolysis, physicochemical characteristics and bioactivity

“…Protease used for enzymatic hydrolysis was from Bacillus licheniformis (Novozyme Corps, Bagsvaerd, Denmark). All analytical grade chemicals used for analysis were purchased from Sigma-Aldrich, Dorset, United Kingdom, unless otherwise stated Enzymatic hydrolysis of EBN Enzymatic hydrolysis was carried out according to the method described by Ibrahim Khushairay et al 1 with modifications. Approximately 3 g of EBN clean and EBN coP were soaked in 100 mL of distilled water in a 250 mL conical flask and maintained at 4 ± 1°C for 16 h. Subsequently, the solutions were double boiled at 90 ± 1°C for 30 min and then cooled to 60 ± 1°C.…”

“…Edible bird's nest (EBN), made up of swiftlet's saliva, is usually harvested from caves or farmhouses and undergoes a cleaning process before being produced into a highly priced edible form. 1 Raw unprocessed EBN contains as much as 90-95% edible part and 5-10% feathers and dirt. 2 According to Marcone, 3 the major components in EBN are protein (62-63%) and carbohydrate (25.6-27.8%), and a low amount of lipid (0.14-1.28%) and ash (2.1%).…”

Characterization and Extraction Influence Protein Profiling of Edible Bird’s Nest

“…Enzymatic hydrolysis is often used to improve the solubility of the protein, and the protein degradation results in the formation of amino acids and peptides with smaller molecular masses easily absorbed by the digestive system. However, strong peptide-peptide interactions and the presence of glycoprotein that cannot be hydrolyzed result in less than 100% protein solubility [38]. Pepsin is a proteolytic enzyme secreted by the stomach, while pancreatin is produced by the pancreas, which contains other enzymes such as elastase, trypsin, and chymotrypsin proteases.…”

Edible Bird’s Nest: Recent Updates and Industry Insights Based On Laboratory Findings

“…After the extraction process, the suspensions are centrifuged at 18,000 rpm for 20 min, and the supernatants are thoroughly dialysed against distilled water (Hun et al, 2016). For enzymatic treatment, enzymes such as pancreatin, alcalase (Khushairay et al, 2014), protease from Bacillus licheniformis (Ling et al, 2020), and neuraminidase from C. perfringens (Guo et al, 2006) is used. In continuation of the heating, the enzyme is usually added to the EBN mixture at a warm temperature between 38°C-60°C, at pH 8.5-9.0 to facilitate partial protein denaturation.…”

“…A widely studied enzyme, pancreatin is a mixture of digestive enzymes that is secreted from the pancreas with proteolytic, amylolytic and lipolytic activities. Treatment with pancreatin significantly increases the protein's solubility, degree of hydrolysis and bioactive peptides concentration of the mixture (Khushairay et al, 2014). For example, pancreatin-treated EBN has higher efficiency compared to non-pancreatin treated EBN in reducing cell death in 6-OHDA-challenged SH-SY5Y cells.…”