Structure of the Thiazole Biosynthetic Enzyme THI1 from Arabidopsis thaliana

Godoi, Paulo H.; Galhardo, Rodrigo S.; Luche, Douglas D.; Sluys, Marie‐Anne Van; Menck, Carlos Frederico Martins; Oliva, Glaucius

doi:10.1074/jbc.m604469200

Cited by 58 publications

(67 citation statements)

References 42 publications

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“…The region spans three BACs and contains seven genes with predicted ORFs (bottom). The thi2-blk Mutant Is Deficient in Thiamine Accumulation THI2 homologs from yeast (THI4) and Arabidopsis (THI1) copurify with ADT, a thiazole precursor to thiamine (Godoi et al, 2006;Jurgenson et al, 2006). Furthermore, the maize THI2 paralog, THI1, rescues the growth defects of yeast thi4 mutants, suggesting a conserved function during thiamine biosynthesis (Belanger et al, 1995).…”

Section: Thi2 Is a Plastid-localized Proteinmentioning

confidence: 99%

A Maize Thiamine Auxotroph Is Defective in Shoot Meristem Maintenance

et al. 2010

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Plant shoots undergo organogenesis throughout their life cycle via the perpetuation of stem cell pools called shoot apical meristems (SAMs). SAM maintenance requires the coordinated equilibrium between stem cell division and differentiation and is regulated by integrated networks of gene expression, hormonal signaling, and metabolite sensing. Here, we show that the maize (Zea mays) mutant bladekiller1-R (blk1-R) is defective in leaf blade development and meristem maintenance and exhibits a progressive reduction in SAM size that results in premature shoot abortion. Molecular markers for stem cell maintenance and organ initiation reveal that both of these meristematic functions are progressively compromised in blk1-R mutants, especially in the inflorescence and floral meristems. Positional cloning of blk1-R identified a predicted missense mutation in a highly conserved amino acid encoded by thiamine biosynthesis2 (thi2). Consistent with chromosome dosage studies suggesting that blk1-R is a null mutation, biochemical analyses confirm that the wild-type THI2 enzyme copurifies with a thiazole precursor to thiamine, whereas the mutant enzyme does not. Heterologous expression studies confirm that THI2 is targeted to chloroplasts. All blk1-R mutant phenotypes are rescued by exogenous thiamine supplementation, suggesting that blk1-R is a thiamine auxotroph. These results provide insight into the role of metabolic cofactors, such as thiamine, during the proliferation of stem and initial cell populations.

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Section: Thi2 Is a Plastid-localized Proteinmentioning

confidence: 99%

A Maize Thiamine Auxotroph Is Defective in Shoot Meristem Maintenance

et al. 2010

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“…While the reaction catalyzed by the thiazole synthase (THI4) has not yet been fully reconstituted, the enzyme copurified with three tightly bound metabolites (35, 40 and 41) and the structure of the enzyme complexed to 41 has been determined. [12][13][14][15] The identity of these metabolites suggested that NAD is the precursor to the thiazole. This was confirmed by the identification of three partial reactions catalyzed by the C204A mutant of THI4 (28 to 29, 29 to 30 and 29 to 35 in Figure 5).…”

Section: Thiamin Biosynthesis In Saccharomyces Cerevisiaementioning

confidence: 99%

Cofactor biosynthesis—still yielding fascinating new biological chemistry

Begley

Chatterjee

Hanes

et al. 2008

Current Opinion in Chemical Biology

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“…Em Arabidopis thaliana, o gene thi1 está relacionado com a via da biossíntese de tiamina, sendo composto por uma ORF de 1047 pares de bases, e codifica uma proteína de 349 resíduos de aminoácidos (9). A dedução da sequência polipeptídica da proteína THI1 revelou um alto grau de identidade com as proteínas envolvidas na biossíntese de tiamina em Saccharomyces cerevisiae (51,7% de identidade) e Schizosaccharomyces pombe (55,1% de identidade) (10).…”

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DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

Santana

Silva

Bernardes

2017

Infarma

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Thiamine functions as a cofactor for activity of various enzymes that act on the metabolism of carbohydrates and amino acids. This study aimed to analyze through bioinformatic techniques the genes responsible for the biosynthesis of thiamine encoded by the genome of Theobroma cacao L. We found two genes, one in chromosome 6 and one in chromosome 7, being appointed as TcTIA6 and TcTIA7. The multiple alignment revealed a strong identity with the Arabidopsis thaliana protein and its direction is destined to the secretory route of the chloroplast. Conserved motifs in the C-terminal region were found, indicating participation in the DNA repair mechanism. Analyzing the dendrogram it was evident that the genes studied had a common ancestor with Gossypium raimondii. The analysis showed that the TcTIA6 and TcTIA7 proteins are probably bifunctional, since they are involved in the biosynthesis of thiamine and stability of the DNA molecule.Key Words: cacao, computational biology, genome, protein, thiamine RESUMO A tiamina funciona como um cofator para atividades de várias enzimas que atuam no metabolismo de carboidratos e aminoácidos. Este trabalho objetivou analisar através de técnicas de bioinformática os genes responsáveis pela biossíntese da tiamina codificadas pelo genoma do Theobroma cacao L. Foram encontrados dois genes, um no cromossomo 6 e outro no 7, sendo nomeados TcTIA6 e TcTIA7. O alinhamento múltiplo da TcTIA6 e TcTIA7 revelou alta identidade com a proteína da Arabidopis thaliana e seu direcionamento é destinado para a rota secretora do cloroplasto. Foram observados motifs conservados na região C-terminal, indicando participação no mecanismo de reparo de DNA. Analisando o dendrograma ficou evidenciado que os genes estudados tiveram um ancestral comum com Gossypium raimondii. As análises mostraram que as proteínas TcTIA6 e TcTIA7 provavelmente são bifuncionais, uma vez que estão envolvidas na biossíntese da tiamina e na estabilidade da molécula de DNA.

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Structure of the Thiazole Biosynthetic Enzyme THI1 from Arabidopsis thaliana

Cited by 58 publications

References 42 publications

A Maize Thiamine Auxotroph Is Defective in Shoot Meristem Maintenance

A Maize Thiamine Auxotroph Is Defective in Shoot Meristem Maintenance

Cofactor biosynthesis—still yielding fascinating new biological chemistry

DUAS PROTEÍNAS (TcTIA6 e TcTIA7) ENVOLVIDAS NA BIOSSÍNTESE DA TIAMINA CODIFICADAS PELO GENOMA do Theobroma cacao L

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