Structure of the human apolipoprotein D gene promoter region

Lambert, Jacques; Provost, Pierre R.; Marcel, Yves L.; Rassart, Éric

doi:10.1016/0167-4781(93)90292-l

Cited by 39 publications

(33 citation statements)

References 27 publications

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“…The Apo D promoter contains several potential regulatory elements like serum, acute phase or stress response elements (SRE, APRE, STRE), an alternating purine-pyrimidine stretch implicated in the response to growth arrest, activation proteins 1 and 2 (AP-1, AP-2) and nuclear factor kappa B (NF-kB) binding sites that may be implicated in Apo D responses (Lambert et al, 1993;Levros et al, 2010). Moreover, it has been shown that some Apolipoprotein E (Apo E) isoforms, E3 and E4, can bind and regulate the Apo D promoter activity (Levros et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Apolipoprotein D subcellular distribution pattern in neuronal cells during oxidative stress

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Apolipoprotein D subcellular distribution pattern in neuronal cells during oxidative stress

et al. 2015

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“…Human ApoD and ApoE Promoter-luciferase Constructs-Heterologous luciferase (LUC) reporter gene constructs containing progressive deletions of the apoD gene 5Ј-flanking region were made by classic methods. The cloning of 10 kbp of upstream sequences has already been reported (40). The Ϫ1176 construct was first digested by combinations of restriction endonucleases as shown in Figs.…”

Section: Methodsmentioning

confidence: 99%

“…The apoD promoter region and non-coding first exon contain a number of potential regulatory and responsive elements that may be important modulators of apoD mRNA expression (20,40). Here, we report a functional analysis of the 5Ј-flanking region of the apoD gene that was designed to identify elements that are important in the induction of apoD gene expression in cells that undergo growth arrest provoked by serum deprivation.…”

mentioning

confidence: 99%

Modulation of Apolipoprotein D and Apolipoprotein E mRNA Expression by Growth Arrest and Identification of Key Elements in the Promoter

Carmo

Séguin

Milne

et al. 2002

Journal of Biological Chemistry

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Apolipoprotein D (apoD) and apolipoprotein E (apoE) are co-expressed in many tissues, and, in certain neuropathological situations, their expression appears to be under coordinate regulation. We have previously shown that apoD gene expression in cultured human fibroblasts is up-regulated when the cells undergo growth arrest. Here, we demonstrate that, starting around day 2 of growth arrest, both apoD and apoE mRNA levels increase between 1.5-and 27-fold in other cell types, including mouse primary fibroblasts and fibroblast-like and human astrocytoma cell lines. To understand the regulatory mechanisms of apoD expression, we have used apoD promoter-luciferase reporter constructs to compare gene expression in growing cells and in cells that have undergone growth arrest. Analysis of gene expression in cells transfected with constructs with deletions and mutations in the apoD promoter and constructs with artificial promoters demonstrated that the region between nucleotides ؊174 and ؊4 is fully responsible for the basal gene expression, whereas the region from ؊558 to ؊179 is implicated in the induction of apoD expression following growth arrest. Within this region, an alternating purine-pyrimidine stretch and a pair of serum-responsive elements (SRE) were found to be major determinants of growth arrest-induced apoD gene expression. Evidence is also presented that SREs in the apoE promoter may contribute to the up-regulation of apoE gene expression following growth arrest.

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“…Although the specific role of this apolipoprotein is unknown, studies in Drosophila (Muffat et al 2008;Sanchez et al 2006) and mouse (Ganfornina et al 2008) ascribe to apo D a key role in longevity regulation and higher survival rate, taking part in the control of lipoperoxidation. It is worthy to note the presence of estrogen response elements (EREs) in the promoter of apo D gene (Lambert et al 1993) and the fact that, in breast cancer cell cultures, estrogens administration leads to a decrease in apo D synthesis concomitant with an increase in cellular proliferation (Simard et al 1991). All these previous data make it very interesting to study in vivo evaluation of estrogen effect on the amount of apo D.…”

Section: Discussionmentioning

confidence: 99%

“…There are several response elements in the Apo D promoter (Lambert et al 1993), so a great number of molecules such as sexual hormones, glucocorticoids, interleukins, retinoic acid, are able to modify the apolipoprotein synthesis (Do Carmo et al 2002;Khan et al 2003;Rassart et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Age-related changes of apolipoprotein D expression in female rat central nervous system with chronic estradiol treatment

Pérez

Navarro

Martínez

et al. 2011

AGE

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Aging is associated with a reduction in metabolic functions, increased incidence of neurodegenerative diseases, and memory or cognitive dysfunction. With aging, a decrease in plasma estrogen levels, related to loss of gonadal function, occurs in females. Estrogens have neuroprotective effects and estradiol treatment improves some aspects of neuronal homeostasis affected by aging. In other way, recent studies show that apo D can play a neuroprotective role in some neuropathologies and during aging. The possible relation between estradiol treatment and the expression of apo D, during aging in the CNS, was investigated in female rats. Our results confirm an expression of apo D zone-dependent, in relation with aging, and an overexpression of apo D related to ovariectomy and estradiol treatment. This overexpression strengthens the idea that apo D plays a neuroprotective role in the CNS.

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Structure of the human apolipoprotein D gene promoter region

Cited by 39 publications

References 27 publications

Apolipoprotein D subcellular distribution pattern in neuronal cells during oxidative stress

Apolipoprotein D subcellular distribution pattern in neuronal cells during oxidative stress

Modulation of Apolipoprotein D and Apolipoprotein E mRNA Expression by Growth Arrest and Identification of Key Elements in the Promoter

Age-related changes of apolipoprotein D expression in female rat central nervous system with chronic estradiol treatment

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