1992
DOI: 10.1021/bi00130a007
|View full text |Cite
|
Sign up to set email alerts
|

Structure of the gene for human coagulation factor V

Abstract: Activated factor V (Va) serves as an essential protein cofactor for the conversion of prothrombin to thrombin by factor Xa. Analysis of the factor V cDNA indicates that the protein contains several types of internal repeats with the following domain structure: A1-A2-B-A3-C1-C2. In this report we describe the isolation and characterization of genomic DNA coding for human factor V. The factor V gene contains 25 exons which range in size from 72 to 2820 bp. The structure of the gene for factor V is similar to the… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
113
0
4

Year Published

1996
1996
2014
2014

Publication Types

Select...
6
3

Relationship

0
9

Authors

Journals

citations
Cited by 184 publications
(117 citation statements)
references
References 47 publications
0
113
0
4
Order By: Relevance
“…The BR and AR are separated in the primary sequence of human FV by ϳ480 amino acids. Interestingly, approximately half of this region has 31 copies of 9-amino acid tandem repeats (9,45,46). However, lower vertebrates such as lizard, chicken, and pufferfish do not have any repeat segments, and the length separating the BR and AR is very short at Ͻ55 residues in these species.…”
Section: Discussionmentioning
confidence: 99%
“…The BR and AR are separated in the primary sequence of human FV by ϳ480 amino acids. Interestingly, approximately half of this region has 31 copies of 9-amino acid tandem repeats (9,45,46). However, lower vertebrates such as lizard, chicken, and pufferfish do not have any repeat segments, and the length separating the BR and AR is very short at Ͻ55 residues in these species.…”
Section: Discussionmentioning
confidence: 99%
“…13 The magnification and the digestion of DNA were carried out according to what was previously described. 14 Briefly, the PCR RFLP (Polymerase Chain Reaction, Restriction Fragment Length Polymorphism) was carried out with the primer 1: 5'-TGCC CAGT GCTTA ACAA GACCA-3' and primer 2: 5'-TGTTATCACACTGGTGCTAA-3'.…”
Section: Genetic Analysis From G1691a Mutationmentioning
confidence: 99%
“…In the inactive form FV acts as APC co-factor in the regulatory activity of FV, IIIa. In inherited and acquired defects, this double role allows FV to influence the manifestation of these disorders into hemorrhagic or thrombotic forms 47,48 . To determine this manifestation, it is necessary to recognize precisely the procoagulation and anticoagulation forms of FV.…”
Section: Pathophysiology Of Action Fv Leiden Mutationmentioning
confidence: 99%