Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465

Zdorovenko, Evelina L.; Качала, В. В.; Sidarenka, A. V.; Ижик, А. В.; Kisileva, Elena P.; Shashkov, Alexander S.; Новик, Г. И.; Knirel, Yuriy A.

doi:10.1016/j.carres.2009.08.039

Cited by 21 publications

(8 citation statements)

References 10 publications

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“…This is a novel structure and differs to those of the EPS structures that have been reported for B. bifidum BIM B-465 12 and B. longum JBL05 13 and is also different to the EPSs that have been isolated and characterised from LAB 15 .…”

Section: Structure Of the Hmw-eps Polymer Synthesised By Strain Ipla-r1contrasting

confidence: 71%

“…Whilst there are a number of reports of EPS producing bifidobacteria, very little work has been undertaken to fully characterise the EPS that they produce, as well as their genetic determinants. The structures of the EPS produced by B. bifidum BIM B-465 12 and B. longum JBL05 13 both of human origin, have recently been reported. But, as far as we know, the functional characterization of genes coding for enzymes involved in EPS-synthesis in Bifidobacterium has not been undertaken to date.…”

Section: Introductionmentioning

confidence: 99%

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Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster

Leivers

Hidalgo-Cantabrana

Robinson

et al. 2011

Carbohydrate Research

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The bile adapted strain Bifidobacterium animalis subsp. lactis IPLA-R1 secretes a high molecular weight exopolysaccharide (HMW-EPS) when grown on the surface of agar-MRSC. This EPS is composed of L-rhamnopyranosyl, D-glucopyranosyl, D-galactopyranosyl and D-galactofuranosyl residues in the ratio of 3:1:1:1. Linkage analysis and 1D and 2D NMR spectroscopy were used to show that the EPS has a hexasaccharide repeating unit with the following structure: [See formula in text]. Treatment of the EPS with mild acid cleanly removed the terminal d-galactofuranosyl residue. The eps cluster sequenced for strain IPLA-R1 showed high genetic homology with putative eps clusters annotated in the genomes of strains from the same species. It is of note that several genes coding for rhamnose-precursors are present in the eps cluster, which could be correlated with the high percentage of rhamnose detected in its EPS repeated unit.

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Section: Structure Of the Hmw-eps Polymer Synthesised By Strain Ipla-r1contrasting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster

Leivers

Hidalgo-Cantabrana

Robinson

et al. 2011

Carbohydrate Research

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“…It has been reported that species of Bifidobacterium , including B. infantis , are able to produce exopolysaccharides (EPS) and their structures have been elucidated [48], [49], [50], [51], [52], [53], [54]. Indeed, Tone-Shimokawa et al revealed that the EPS produced by the B. infantis ATCC 15697 (formally referred as B. infantis Reuteri ATCC 15697) is a galactose polymer with the structure of β-D-Gal-(1→3)-α-D-Gal [55].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans

Kim

Garrido

et al. 2013

PLoS ONE

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Bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable of metabolizing human milk oligosaccharides (HMO). To investigate the bacterial response to different prebiotics, we analyzed both cell wall associated and whole cell proteins in B. infantis. Proteins were identified by LC-MS/MS followed by comparative proteomics to deduce the protein localization within the cell. Enzymes involved in the metabolism of lactose, glucose, galactooligosaccharides, fructooligosaccharides and HMO were constitutively expressed exhibiting less than two-fold change regardless of the sugar used. In contrast, enzymes in N-Acetylglucosamine and sucrose catabolism were induced by HMO and fructans, respectively. Galactose-metabolizing enzymes phosphoglucomutase, UDP-glucose 4-epimerase and UTP glucose-1-P uridylytransferase were expressed constitutively, while galactokinase and galactose-1-phosphate uridylyltransferase, increased their expression three fold when HMO and lactose were used as substrates for cell growth. Cell wall-associated proteomics also revealed ATP-dependent sugar transport systems associated with consumption of different prebiotics. In addition, the expression of 16 glycosyl hydrolases revealed the complete metabolic route for each substrate. Mucin, which possesses O-glycans that are structurally similar to HMO did not induced the expression of transport proteins, hydrolysis or sugar metabolic pathway indicating B. infantis do not utilize these glycoconjugates.

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“…Structural information for only four EPSs from bifidobacteria have been published: two from Bifidobacterium longum (Altmann et al 2016, Inturri et al 2017, Kohno et al 2009 and two from Bifidobacterium animalis (Leivers et al 2011, Shang et al 2013. A further nine papers have described a variety of cell-wall polysaccharides recovered from strains of bifidobacteria (Fischer Werner 1987a, Habu et al 1987, Nagaoka et al 1988, Nagaoka et al 1995, Nagaoka et al 1996, Tone-Shimokawa et al 1996, Uemura and Matsumoto 2014, Valueva et al 2013, Zdorovenko et al 2009 and the structures of two teichoic acids have also been reported (Veerkamp et al 1983). The reason why so few bifidobacterial exocellular polysaccharides have been characterised is because these bacteria are often difficult to grow, they require a complex growth medium and they often produce very small amounts of mixtures of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve

Alhudhud

Sadiq

Ngo

et al. 2018

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Three strains of Bifidobacterium breve (JCM 7017, JCM 7019 and JCM 2258) and two strains of Bifidobacterium animalis subsp. lactis (AD011 and A1dOxR) were grown in broth cultures or on plates, and a standard exopolysaccharide extraction method was used in an attempt to recover exocellular polysaccharides. When the extracted materials were analysed by NMR it was clear that mixtures of polysaccharides were being isolated including exopolysaccharides (EPS) cell wall polysaccharides and intracellular polysaccharides. Treatment of the cell biomass from the B. breve strains, or the B. animalis subsp. lactis AD011 strain, with aqueous sodium hydroxide provided a very similar mixture of polysaccharides but without the EPS. The different polysaccharides were partially fractionated by selective precipitation from an aqueous solution upon the addition of increasing percentages of ethanol. The polysaccharides extracted from B. breve JCM 7017 grown in HBM media supplemented with glucose (or isotopically labelled D-glucose-1-C) were characterised using 1D and 2D-NMR spectroscopy. Addition of one volume of ethanol generated a medium molecular weight glycogen (Mw=1×10 Da, yield 200 mg/l). The addition of two volumes of ethanol precipitated an intimate mixture of a low molecular weight β-(1→6)-glucan and a low molecular weight β-(1→6)-galactofuranan which could not be separated (combined yield 46 mg/l). When labelled D-glucose-1-C was used as a carbon supplement, the label was incorporated into >95% of the anomeric carbons of each polysaccharide confirming they were being synthesised in situ. Similar H NMR profiles were obtained for polysaccharides recovered from the cells of B. animalis subsp. lactis AD011and A1dOxR (in combination with an EPS), B. breve JCM 7017, B. breve JCM 7019, B. breve JCM 2258 and from an EPS (-ve) mutant of B. breve 7017 (a non-EPS producer).

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Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465

Cited by 21 publications

References 10 publications

Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster

Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster

Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans

Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve

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