Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans

Kim, Jae-Han; An, Hyun Joo; Garrido, Daniel; German, J. Bruce; Lebrilla, Carlito B.; Mills, David A.

doi:10.1371/journal.pone.0057535

Cited by 76 publications

(53 citation statements)

References 55 publications

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“…Additionally, strain does not show any preference for glucose and this parallel with a previous study concerning its behaviour relation to galactose, glucose and lactose uptake on the mixture of monosaccharides [18]. …”

Section: Changes Of Carbohydrates In Skim Milk During B Pseudocatenusupporting

confidence: 89%

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2017

The Asia Journal of Applied Microbiology

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Article HistoryGrowth characteristics of Bifidobaceria pseudocatenulatum G4 in low concentration milk medium were studied. During cultivation process, profiles of cell growth, pH changes, organic acids and sugar profile were monitored. A finding showed that final cell concentration (cfumL -1 ) obtained in 2 and 4% (w/v) milk concentration was two times lower than in comparative media. However, 6% (w/v) skim milk gave a promising media for B. pseudocatenulatum G4 growth. Organic acids production (acetic, lactic, formic and citric acid) by strains was detected in all milk medium concentrations and similar profile were observed. Except for formic acid which actively produced in 2 and 4% (w/v) milk concentration. The accumulation of formic acids was believed due to carbon limitation. As for sugar profile, co-metabolized galactose and glucose were observed in 2, 4 and 6% (w/v) skim milk concentration. Meanwhile high carbon remains occurred in 8 and 10% (w/v) milk concentrations after cultivation period. Among all concentrations, milk with 6% (w/v) concentration showed better support for cells growth without formic acid presence and low carbon remains at the end of cultivation period.

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Section: Changes Of Carbohydrates In Skim Milk During B Pseudocatenusupporting

confidence: 89%

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2017

The Asia Journal of Applied Microbiology

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“…This compound is abundant in plasma but not observed in either bovine or human milk. We have previously shown that there are glycosidases produced by commensal bacteria in the infant gut [30], therefore we may speculate that U-SLN and other sialylated compounds can be produced by the interaction of milk, milk products and bacterial enzymes, i.e. an in vivo example of a new oligosaccharide structure produced by the gut microbiota.…”

Section: Resultsmentioning

confidence: 99%

Detection of milk oligosaccharides in plasma of infants

et al. 2014

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Human Milk Oligosaccharides (HMO) are one of the major components of human milk. HMO are non-digestible by the human gut, where they are known to play important functions as prebiotics and decoys for binding pathogens. Moreover, it has been proposed that HMO may provide sialic acids to the infant that are important in brain development, however this would require absorption of HMO into the bloodstream. HMO have consistently been found in the urine of humans and other mammals, suggesting systemic absorption. Here we present a procedure for the profiling of milk oligosaccharides (MO) in plasma samples obtained from 13 term infants hospitalized for surgery for congenital heart disease. The method comprises protein denaturation, oligosaccharide reduction and porous graphitized carbon solid phase extraction for purification followed by analysis using nHPLC-PGC-chip-TOF-MS. Approximately 15 free MO were typically observed in the plasma of human infants, including LNT, LDFP, LNFT, 3’SL, 6’SL, 3’SLN and 6’SLN, of which the presence was confirmed using fragmentation studies. A novel third isomer of SLN, not found in human or bovine milk was also consistently detected. Differences in the free MO profiles were observed between infants that were totally formula-fed and infants that received at least some part breast milk. Our results indicate that free MO similar in structure to those found in human milk and urine are present in the blood of infants. The method and results presented here will facilitate further research toward the possible roles of free MO in the development of the infant.

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“…First, transporter proteins that bind to specific HMO linkages, including a number of solute-binding proteins with an affinity for HMOs, are upregulated in B. infantis grown on HMO but not in B. infantis grown on the simpler prebiotic oligosaccharides fructo-oligosaccharide or galacto-oligosaccharide (29,30). This suggests that B. infantis is able to transport intact HMOs into its cytoplasm and that this capacity is “turned on” by the HMOs.…”

Section: Mechanistic Evidence For Colonization By B Infantismentioning

confidence: 99%

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

et al. 2014

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Oligosaccharides are abundant in human milk. Production of these highly diverse structures requires significant energy expenditure by the mother and yet these human milk oligosaccharides offer no direct nutritive value to her infant. A primary function of human milk oligosaccharides is to shape the infant’s intestinal microbiota with life-long consequences. Bifidobacterium longum subspecies infantis (B. infantis) is unique among gut bacteria in its prodigious capacity to digest and consume any human milk oligosaccharide structure, the result of a large repertoire of bacterial genes encoding an array of glycosidases and oligosaccharide transporters not found in other bacterial species. In vitro, B. infantis grows better than other bacterial strains in the presence of human milk oligosaccharides, displays anti-inflammatory activity in premature intestinal cells, and decreases intestinal permeability. In premature infants, B. infantis given in combination with human milk increases B. infantis and decreases Enterobacteriaceae in the feces. Probiotics containing B. infantis decrease the risk of necrotizing enterocolitis in premature infants. Colonization with B. infantis is also associated with increased vaccine responses. Probiotic organisms have historically been selected based on ease of production and stability. The advantages of B. infantis, selected through coevolution with human milk glycans, present an opportunity for focused manipulation of the infant intestinal microbiota.

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Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans

Cited by 76 publications

References 55 publications

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Detection of milk oligosaccharides in plasma of infants

Bifidobacterium longum subspecies infantis: champion colonizer of the infant gut

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