Structure of succinoglycan from an infectious strain of Agrobacterium radiobacter

Characterization of the chemical structure and innate immune-stimulating activity of an extracellular polysaccharide from <i>Rhizobium</i> sp. strain M2 screened using a silkworm muscle contraction assay

“…These results indicate that M2 EPS consists of octasaccharide repeating units. The molar ratio of the detected sugar connectivity was in good agreement with the theoretical ratio of PMAA from succinoglycan, a representative polysaccharide produced by genus Rhizobium (24).…”

“…A pyruvate residue was bound to both O-4 and O-6 positions of the terminal glucose residue of the side-chain of M2 EPS. Although we could not determine the binding position of the succinate in the M2 EPS, succinate groups bind to glucose residues in the side-chains of succinoglycan from various microbial sources (24,25). The glycan structure of the side-chain of deacylated and depyruvylated M2 EPS is a β-1,3/6-glucan with the following structure: β-Glcp-(1→3)-β-Glcp-(1→3)-β-Glcp-(1→6)-β-Glcp-(1→6)-β-Glcp-(1→ Previously, the innate immunostimulating activities of β-1,3/6-glucan from various sources were evaluated using the silkworm muscle contraction assay (9), and the activities varied according to the origin.…”

Relevance of Fucose-Rich Extracellular Polysaccharides Produced by Rhizobium sullae Strains Nodulating Hedysarum coronarium L. Legumes

“…5). The determined chemical shifts were compared to those in the literature (24,(30)(31)(32)(33)(34)(35)(36) C (␦ ϭ 100.1 ppm) and 1 H (␦ ϭ 3.82, 3.88, and 3.70 ppm) corresponding respectively to the two H-6 and to H-3 or H-4 of the hexose. Attribution of the chemical shifts corresponding to the quaternary carbons (C and COOH) of the substituents was performed using the HMBC data.…”

Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling

“…This perspective aims to demonstrate the utility of a combined multiphase NMR and molecular modeling approach for the characterization of two microbial sphingan EPSs: gellan and diutan gum. Gellan has been by far one of the most studied EPS and include X-ray as well as NMR analysis ( Jansson et al, 1983 ; Grasdalen and Smidsrod, 1987 ; Chandrasekaran et al, 1988 ; Bosco et al, 2000 ), whereas only few studies included NMR analysis of diutan ( Evans et al, 2000 ). Even though X-ray analysis provides very detailed information about crystalline samples, multiphase NMR enables structural characterization of molecules present in crystalline and amorphous solids as well as gels and solutions.…”