Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

Bioud, Fatma-Zohra; Gasecka, Paulina; Ferrand, Patrick; Rigneault, Hervé; Duboisset, Julien; Brasselet, Sophie

doi:10.1103/physreva.89.013836

Cited by 31 publications

(86 citation statements)

References 26 publications

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“…In particular, S 2 is equal to 0 in isotropic samples, and increases with increasing order, e.g., decreasing width of the molecular angular distribution excited in the focal volume. Note that the relation between the obtained S 2 ; φ 2 parameters and the intrinsic molecular order taking place within the focal spot of the microscope can be directly related to regular polarization-resolved CARS and SRS [17]. In particular, it can be shown that when the polarization dependence of the measured intensity is truncated to its second-order Fourier series such as done in Eq.…”

Section: Principle Of Fast-polarization Crs Imagingmentioning

confidence: 95%

“…Here we do not represent higher harmonics that could be treated as additional information [17]. S 2 and φ 2 are, respectively, related to the degree of alignment of molecular bonds within the focal spot of the microscope objective and to its average orientation (see Supplement 1).…”

Section: Principle Of Fast-polarization Crs Imagingmentioning

confidence: 99%

“…Using such methods, high molecular organization has been evidenced in water molecules between phospholipid layers [11], CN bonds of liquid crystals [12,13], CH bonds of polyglucan chains in cellulose [14], and in various symmetry modes of zeolite crystals [15]. Monitoring the CH 2 vibrational stretching bond in lipids has shown considerable level of information on lipid order within artificial multilayers [16,17], where the measured modulation was used to retrieve quantitative information on sub-diffraction molecular orientational order behaviors, in particular, its mean orientation and angular distribution shape [17]. Lipid order imaging by polarization-resolved CARS has also been applied in myelin, which forms dense lipid multilayers around axons.…”

Section: Introductionmentioning

confidence: 99%

“…Lipid order imaging by polarization-resolved CARS has also been applied in myelin, which forms dense lipid multilayers around axons. Myelin structural imaging has been demonstrated in fixed tissues in mouse brain [18,19] and sciatic nerve [20], as well as in the spinal cord of mice [17,21] and anesthetized zebrafish [22]. Being able to monitor lipid order without the need of fluorescent labels ultimately brings new knowledge on lipid packing properties, which is critical for the structural integrity of myelin in the central and peripheral nervous systems, with consequences in neuropathologies [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Yet currently developed techniques are not able to address fast time scale dynamics, the main reason being the requirement of point scanning modalities. Typical molecular order imaging is performed at the rate of minutes for sequential polarization tuning on images of 150 × 150 pixels [17,19,21,22], to tens of seconds in schemes using synchronization between line scanning and polarization rotation [20]. This limits the current applicability to minute-scale processes, with the obvious inconvenience of sensitivity to sample motion and lack of dynamics information.…”

Section: Introductionmentioning

confidence: 99%

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High-speed polarization-resolved coherent Raman scattering imaging

Hofer¹,

Balla²,

Brasselet³

2017

Optica

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Polarization-resolved coherent Raman scattering (polar-CRS) provides rich information on molecular orientational organization, with the strong advantages of being a label-free and chemically specific imaging method. Its implementation, however, strongly reduces the imaging acquisition rate, due to limits imposed by polarization tuning. Here we demonstrate fast-polar-CRS imaging based on combined electro-optic polarization and acousto-optic amplitude modulations, applicable to both stimulated Raman scattering and coherent anti-Stokes Raman scattering imaging. The proposed scheme adds polarization information without compromising the capacities of regular CRS intensity imaging; increases the speed of orientational imaging by two orders of magnitude as compared with previous approaches; and does not require post-processing analyses. We show that this method permits sub-second time-scale imaging of lipid order packing and local lipid membrane deformations in artificial lipid multilayers, but also in red blood cell ghosts, demonstrating its high sensitivity down to a single lipid bilayer membrane.

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Section: Principle Of Fast-polarization Crs Imagingmentioning

confidence: 95%

Section: Principle Of Fast-polarization Crs Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High-speed polarization-resolved coherent Raman scattering imaging

Hofer¹,

Balla²,

Brasselet³

2017

Optica

Self Cite

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Maintaining polarization in polarimetric multiphoton microscopy

Bélanger

Turcotte

Daradich

et al. 2015

Journal of Biophotonics

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Polarimetric measurements in multiphoton microscopy can reveal information about the local molecular order of a sample. However, the presence of a dichroic through which the excitation beam propagates will generally scramble its polarization. We propose a simple scheme whereby a second properly-oriented compensation dichroic is used to negate any alteration regardless of the wavelength and the initial polarization. We demonstrate how this robust and rapid approach simplifies polarimetric measurements in second-harmonic generation, two-photon excited fluorescence and coherent anti-Stokes Raman scattering. Illustration of the polarization maintaining strategy with the compensating dichroic oriented such that its s- and p-axes are interchanged with these of the primary dichroic.

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RP‐CARS reveals molecular spatial order anomalies in myelin of an animal model of Krabbe disease

Vito

Cappello

Tonazzini

et al. 2016

Journal of Biophotonics

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Krabbe disease (KD) is a rare demyelinating sphingolipidosis, often fatal in the first years of life. It is caused by the inactivation of the galactocerebrosidase (GALC) enzyme that causes an increase in the cellular levels of psychosine considered to be at the origin of the tissue-level effects. GALC is inactivated also in the Twitcher (TWI) mouse: a genetic model of KD that is providing important insights into the understating of the pathogenetic process and the development of possible treatments. In this article an innovative optical technique, RP-CARS, is proposed as a tool to study the degree of order of the CH bonds inside the myelin sheaths of TWI-mice sciatic-nerve fibres. RP-CARS, a recently developed variation of CARS microscopy, is able to combine the intrinsic chemical selectivity of CARS microscopy with molecular-bond-spatial-orientation sensibility. This is the first time RP-CARS is applied to the study of a genetic model of a pathology, leading to the demonstration of a post-onset progressive spatial disorganization of the myelin CH bonds. The presented result could be of great interest for a deeper understanding of the pathogenic mechanisms underlying the human KD and, moreover, it is an additional proof of the experimental validity of this microscopy technique. RP-CARS image (2850 cm , CH bonds) of a sciatic-nerve optical longitudinal section from a Twitcher P23 (symptomatic) mouse. Scale bar: 10 microns. The image was constructed by colour-mapping the degree of molecular order of the CH bonds inside the myelin walls, as displayed in the colour bar on the right.

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Structure of molecular packing probed by polarization-resolved nonlinear four-wave mixing and coherent anti-Stokes Raman-scattering microscopy

Cited by 31 publications

References 26 publications

High-speed polarization-resolved coherent Raman scattering imaging

High-speed polarization-resolved coherent Raman scattering imaging

Maintaining polarization in polarimetric multiphoton microscopy

RP‐CARS reveals molecular spatial order anomalies in myelin of an animal model of Krabbe disease

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