Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes

Rädler, Joachim O.; Koltover, Ilya; Salditt, Tim; Safinya, Cyrus R.

doi:10.1126/science.275.5301.810

Cited by 1,371 publications

(1,435 citation statements)

References 28 publications

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“…The sequence of events outlined above leads to a strong electrostatic attraction. This type of electrostatic interaction has been observed for self-assembled complexes between membranes and a variety of anionic polymers, including DNA [64][65][66], F-actin [67], microtubules [68], and filamentous phages [69].…”

Section: Electrostaticsmentioning

confidence: 76%

Arginine‐rich cell‐penetrating peptides

et al. 2009

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a b s t r a c tArginine-rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still under investigation. Recent dialogue has centered on a debate over the roles that direct translocation and endocytotic pathways play in internalization of cell-penetrating peptides. In this paper, we review the evidence for the broad range of proposed mechanisms, and show that each distinct process requires negative Gaussian membrane curvature as a necessary condition. Generation of negative Gaussian curvature by cell-penetrating peptides is directly related to their arginine content. We illustrate these concepts using HIV TAT as an example.

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Section: Electrostaticsmentioning

confidence: 76%

Arginine‐rich cell‐penetrating peptides

et al. 2009

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“…33 The population(s) of complexes, which actually transfect cells is still uncertain with several models proposed, such as beads on a string, 34 spaghetti and meatballs 33 and multilamellar sheets. 35 However, there is also convincing evidence to support the existence of macro-aggregates within each of these populations. 33 Several factors favour macro-aggregate formation such as the impurity of the DNA, the composition of the suspending vehicle and the ratio of cationic liposome to DNA.…”

Section: Discussionmentioning

confidence: 99%

β-Galactosidase staining following intracoronary infusion of cationic liposomes in the in vivo rabbit heart is produced by microinfarction rather than effective gene transfer: a cautionary tale

et al. 1998

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The myocardium is a potential target for the expression of conversion at day 3 with associated myocardial damage. exogenous genes to treat inherited and acquired diseases.We hypothesised that the damage was associated with Although adenovirus-mediated gene transfer has resulted macro-aggregates of cationic liposomes-DNA occluding in high-level gene transfer in vivo via direct intramyocardial the microcirculation. When such aggregates were excluded injection and via a percutaneous intra-arterial route, the no X-gal conversion was seen in vivo. In order to show time-course of gene expression is limited by host immune that X-gal conversion occurs in areas of infarction in the responses. It was the aim of this study to test whether catmyocardium we caused closed chest infarction by ionic liposome-mediated gene transfer, which does not sufdeploying a platinum micro-embolisation coil in the circumfer from the aforementioned problems, was feasible in the flex coronary artery. At day 3 X-gal conversion was adult rabbit myocardium via a percutaneous transluminal observed in the territory supplied by the occluded artery. approach. Doses of plasmid DNA encoding lacZ from 200-Thus, microinfarction causes the false positive appearance 800 g complexed to cationic liposomes resulted in X-gal of gene transfer when using a lacZ reporter gene.

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“…We use liposome-mediated gene transfaction 8,9 to either block maternal Hoxa10 expression with a Hoxa10 antisense oligonucleotide or to increase maternal Hoxa10 expression with a plasmid that constitutively expresses Hoxa10. These experiments demonstrate that manipulation of adult Hoxa10 expression significantly affects fertility.…”

Section: Introductionmentioning

confidence: 99%

Alteration of maternal Hoxa10 expression by in vivo gene transfection affects implantation

2000

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Mice with a targeted mutation of the Hoxa10 gene demonstrate uterine factor infertility. It is unclear if the defect in the uterine environment arises due to the absence of Hoxa10 expression during embryonic development or in the adult. We have recently demonstrated that HOXA10 expression in human endometrium rises dramatically at the time of implantation, suggesting maternal expression of Hoxa10/HOXA10 may be essential to the process. To assess the importance of maternal Hoxa10 expression, the uteri of day 2 pregnant mice were injected with a DNA/liposome complex containing constructs designed to alter maternal Hoxa10 expression before implantation. Transfection with a Hoxa10 antisense oligodeoxyribonucleotide significantly decreased the number

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Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes

Cited by 1,371 publications

References 28 publications

Arginine‐rich cell‐penetrating peptides

Arginine‐rich cell‐penetrating peptides

β-Galactosidase staining following intracoronary infusion of cationic liposomes in the in vivo rabbit heart is produced by microinfarction rather than effective gene transfer: a cautionary tale

Alteration of maternal Hoxa10 expression by in vivo gene transfection affects implantation

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