Structure of a Plasmodium chabaudi acidic phosphoprotein that is associated with the host erythrocyte membrane

Deleersnijder, Willy; Prasomsitti, Pornthip; Tungpradubkul, Sumalee; Hendrix, Diana; Hamers-Casterman, C; Hamers, R.

doi:10.1016/0166-6851(92)90154-c

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…Genes 1w (1591–2482) and 14w (54456–56509) are closely related despite their differences in size. They show similarities to the Pc EMA1 gene (Favaloro and Kemp, 1994) and to the Pc acidic phosphoprotein gene (Deleersnijder et al ., 1992), both thought to encode erythrocyte surface proteins. We detected at least 12 subtelomeric fragments containing members of this family.…”

Section: Resultsmentioning

confidence: 99%

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Fischer

Chavchich

Huestis

et al. 2003

Molecular Microbiology

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SummaryThe chromosome ends of human malaria parasites harbour many genes encoding proteins that are exported to the surface of infected red cells, often being involved in host-parasite interactions and immune evasion. Unlike other murine malaria parasites Plasmodium chabaudi undergoes antigenic variation during passage in the laboratory mouse and hence is a model suitable for investigation of switching mechanisms. However, little is known about the subtelomeric regions of P. chabaudi chromosomes and its variable antigens. Here we report 80 kb of sequence from an end of one P. chabaudi chromosome. Hybridization of probes spanning this region to two dimensional pulsed field gels of the genome revealed 10 multicopy gene families located exclusively in subtelomeric regions of multiple P. chabaudi chromosomes, interspersed amongst multicopy intergenic regions. Hence all chromosomes share a common subtelomeric structure, presumably playing a similar role in spatial positioning as the P. falciparum Rep20 sequence. Expression in blood stages, domains characteristic of surface antigens and copy numbers between four and several hundred per genome, indicate a functional role in antigenic variation for some of these families. We identify members of the cir family, as well as novel genes, that although clearly homologous to cir have large low complexity regions in the predicted extracellular domains. Although all families have homologues in other rodent Plasmodium species, four were previously not known to be subtelomeric. Six have homologues in human and simian malarias.

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Section: Resultsmentioning

confidence: 99%

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Fischer

Chavchich

Huestis

et al. 2003

Molecular Microbiology

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“…Co-occurrence of PYST-A and PcEMA1 domains within the same polypeptide suggests physical interaction between PYST-A and PcEMA1 proteins. This possibility is supported by the fact that PcEMA1 proteins also localize to the cytoplasmic face of the RBC membrane [58]. It remains to be shown why rodent malaria parasites maintain so many PYST-A proteins compared to primate malaria parasites.…”

Section: Discussionmentioning

confidence: 99%

Variant surface antigens of malaria parasites: functional and evolutionary insights from comparative gene family classification and analysis

Frech

Chen

2013

BMC Genomics

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BackgroundPlasmodium parasites, the causative agents of malaria, express many variant antigens on cell surfaces. Variant surface antigens (VSAs) are typically organized into large subtelomeric gene families that play critical roles in virulence and immune evasion. Many important aspects of VSA function and evolution remain obscure, impeding our understanding of virulence mechanisms and vaccine development. To gain further insights into VSA function and evolution, we comparatively classified and examined known VSA gene families across seven Plasmodium species.ResultsWe identified a set of ultra-conserved orthologs within the largest Plasmodium gene family pir, which should be considered as high-priority targets for experimental functional characterization and vaccine development. Furthermore, we predict a lipid-binding domain in erythrocyte surface-expressed PYST-A proteins, suggesting a role of this second largest rodent parasite gene family in host cholesterol salvage. Additionally, it was found that PfMC-2TM proteins carry a novel and putative functional domain named MC-TYR, which is conserved in other P. falciparum gene families and rodent parasites. Finally, we present new conclusive evidence that the major Plasmodium VSAs PfEMP1, SICAvar, and SURFIN are evolutionarily linked through a modular and structurally conserved intracellular domain.ConclusionOur comparative analysis of Plasmodium VSA gene families revealed important functional and evolutionary insights, which can now serve as starting points for further experimental studies.

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“…1990) ; Y is a cDNA clone (n° 110) of 1.1 kb corresponding to a schizont-specific protein ; X is a genomic DNA clone (X2A) of 2.3 kb corres ponding to a part of a phosphoprotein associated with the erythrocyte cell membrane (Deleersnijder et al, 1992).…”

Section: Dna Probesmentioning

confidence: 99%

Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

1994

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Summary :The molecular karyotypes of the African murine malaria parasites P. berghei (3 strains, 2 lines) P. yoelii (2 strains) P. chabaudi (3 strains, 1 line) and P. vinckei (4 strains) have been studied using orthogonal field alternation gel electrophoresis (OFAGE).The genome of each species was resolved into 9 to 11 distinct chromosomal DNA bands molecules of varying intensities which seem to represent 14 chromosomes ranging in size from 600 kb

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Structure of a Plasmodium chabaudi acidic phosphoprotein that is associated with the host erythrocyte membrane

Cited by 9 publications

References 19 publications

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Ten families of variant genes encoded in subtelomeric regions of multiple chromosomes of Plasmodium chabaudi, a malaria species that undergoes antigenic variation in the laboratory mouse

Variant surface antigens of malaria parasites: functional and evolutionary insights from comparative gene family classification and analysis

Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

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