Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

Said, Ahamada; Wéry, M; Hamers, R.

doi:10.1051/parasite/1994011031

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…Maps can also be linked together by a series of double digestions, by the use of available sequence information or by Southern blot using chromosome-specific probes. Because unicellular parasites have relatively small chromosomes that do not visibly condense, PFGE has provided a means by which chromosomal entities can be physically mapped and studied at the molecular level [15][16][17] . In fact, PFGE separations are currently providing the very material that the international malaria consortium is using to create chromosomal-specific libraries for large-scale sequencing efforts (http://www-ermm.…”

Section: Deep Contigs Corresponding To the Molecular Karyotype Determmentioning

confidence: 99%

A shotgun optical map of the entire Plasmodium falciparum genome

et al. 1999

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The unicellular parasite Plasmodium falciparum is the cause of human malaria, resulting in 1.7-2.5 million deaths each year. To develop new means to treat or prevent malaria, the Malaria Genome Consortium was formed to sequence and annotate the entire 24.6-Mb genome. The plan, already underway, is to sequence libraries created from chromosomal DNA separated by pulsed-field gel electrophoresis (PFGE). The AT-rich genome of P. falciparum presents problems in terms of reliable library construction and the relative paucity of dense physical markers or extensive genetic resources. To deal with these problems, we reasoned that a high-resolution, ordered restriction map covering the entire genome could serve as a scaffold for the alignment and verification of sequence contigs developed by members of the consortium. Thus optical mapping was advanced to use simply extracted, unfractionated genomic DNA as its principal substrate. Ordered restriction maps (BamHI and NheI) derived from single molecules were assembled into 14 deep contigs corresponding to the molecular karyotype determined by PFGE (ref. 3).

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Section: Deep Contigs Corresponding To the Molecular Karyotype Determmentioning

confidence: 99%

A shotgun optical map of the entire Plasmodium falciparum genome

et al. 1999

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“…Although these models cannot reflect all aspects of human infections, they allow the study of controlled experimental infections. There are 4 rodent malaria species, P. berghei , P. chabaudi , P. vinckei , and P. yoelii , 13 subspecies, and various strains and cloned lines [18]. These parasites were isolated from African thicket rats in Central and West Africa more than 50 years ago [19].…”

Section: Innate Immunity To Pathogensmentioning

confidence: 99%

Interferons and Interferon Regulatory Factors in Malaria

Gun

Claser

Tan

et al. 2014

Mediators of Inflammation

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Malaria is one of the most serious infectious diseases in humans and responsible for approximately 500 million clinical cases and 500 thousand deaths annually. Acquired adaptive immune responses control parasite replication and infection-induced pathologies. Most infections are clinically silent which reflects on the ability of adaptive immune mechanisms to prevent the disease. However, a minority of these can become severe and life-threatening, manifesting a range of overlapping syndromes of complex origins which could be induced by uncontrolled immune responses. Major players of the innate and adaptive responses are interferons. Here, we review their roles and the signaling pathways involved in their production and protection against infection and induced immunopathologies.

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“…Here we present natural infection of BALB/c mice with the filarial nematode L . sigmodontis ([ 21 ], formerly known as Litomosoides carinii and later re-named by Bain et al . [ 22 ]) as a rodent model for an in vivo screening of anti-wolbachial compounds.…”

Section: Introductionmentioning

confidence: 99%

Combinations of registered drugs reduce treatment times required to deplete Wolbachia in the Litomosoides sigmodontis mouse model

et al. 2018

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Filarial parasites can be targeted by antibiotic treatment due to their unique endosymbiotic relationship with Wolbachia bacteria. This finding has led to successful treatment strategies in both, human onchocerciasis and lymphatic filariasis. A 4–6 week treatment course using doxycycline results in long-term sterility and safe macrofilaricidal activity in humans. However, current treatment times and doxycycline contraindications in children and pregnant women preclude widespread administration of doxycycline in public health control programs; therefore, the search for shorter anti-wolbachial regimens is a focus of ongoing research. We have established an in vivo model for compound screening, using mice infected with Litomosoides sigmodontis. We could show that gold standard doxycycline treatment did not only deplete Wolbachia, it also resulted in a larval arrest. In this model, combinations of registered antibiotics were tested for their anti-wolbachial activity. Administration of rifamycins in combination with doxycycline for 7 days successfully depleted Wolbachia by > 2 log (>99% reduction) and thus resulted in a significant reduction of the treatment duration. Using a triple combination of a tetracycline (doxycycline or minocycline), a rifamycin and a fluoroquinolone (moxifloxacin) led to an even greater shortening of the treatment time. Testing all double combinations that could be derived from the triple combinations revealed that the combination of rifapentine (15mg/kg) and moxifloxacin (2 x 200mg/kg) showed the strongest reduction of treatment time in intraperitoneal and also oral administration routes. The rifapentine plus moxifloxacin combination was equivalent to the triple combination with additional doxycycline (>99% Wolbachia reduction). These investigations suggest that it is possible to shorten anti-wolbachial treatment times with combination treatments in order to achieve the target product profile (TPP) requirements for macrofilaricidal drugs of no more than 7–10 days of treatment.

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Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines

Cited by 4 publications

References 11 publications

A shotgun optical map of the entire Plasmodium falciparum genome

A shotgun optical map of the entire Plasmodium falciparum genome

Interferons and Interferon Regulatory Factors in Malaria

Combinations of registered drugs reduce treatment times required to deplete Wolbachia in the Litomosoides sigmodontis mouse model

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