Structure-induced cell growth by 3D printing of heterogeneous scaffolds with ultrafine fibers

Xie, Chaoqi; Gao, Qing; Wang, Peng; Shao, Lei; Yuan, Huixin; Fu, Jianzhong; Chen, Wei; He, Yong

doi:10.1016/j.matdes.2019.108092

Cited by 113 publications

(106 citation statements)

References 45 publications

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“…As MEW is a quite new technique, research just started looking into the possibility of structure induced cell growth by different orientation of MEW fibers. [59,60] However, an orientated growth of the keratinocytes induced by the MEW fibers was not observed in our study. In future work the cell interaction with radial and circular fibers will be investigated.…”

Section: Biological Responsecontrasting

confidence: 83%

Biomimetic Tympanic Membrane Replacement Made by Melt Electrowriting

Witzleben

Stoppe

Ahlfeld

et al. 2021

Adv Healthcare Materials

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The tympanic membrane (TM) transfers sound waves from the air into mechanical motion for the ossicular chain. This requires a high sensitivity to small dynamic pressure changes and resistance to large quasi-static pressure differences. The TM achieves this by providing a layered structure of about 100µm in thickness, a low flexural stiffness, and a high tensile strength. Chronically infected middle ears require reconstruction of a large area of the TM. However, current clinical treatment can cause a reduction in hearing. With the novel additive manufacturing technique of melt electrowriting (MEW), it is for the first time possible to fabricate highly organized and biodegradable membranes within the dimensions of the TM. Scaffold designs of various fiber composition are analyzed mechanically and acoustically. It can be demonstrated that by customizing fiber orientation, fiber diameter, and number of layers the desired properties of the TM can be met. An applied thin collagen layer seals the micropores of the MEW-printed membrane while keeping the favorable mechanical and acoustical characteristics. The determined properties are beneficial for implantation, closely match those of the human TM, and support the growth of a neo-epithelial layer. This proves the possibilities to create a biomimimetic TM replacement using MEW. IntroductionDefects of the tympanic membrane (TM) have many causes, ranging from injuries to chronic otitis media (COM). In the

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Section: Biological Responsecontrasting

confidence: 83%

Biomimetic Tympanic Membrane Replacement Made by Melt Electrowriting

Witzleben

Stoppe

Ahlfeld

et al. 2021

Adv Healthcare Materials

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show abstract

“…Furthermore, cell proliferation was reported to be greater in small pores than in large pores. [36] Other studies on similar scaffolds have also revealed that cell alignment occurs preferentially along the long axis of oblong shaped pores, [37] and that pore size and suspended fiber sagging influence cell growth. [38] In the present study, 3D suspended fiber scaffolds were shown to support the growth and migration S100-positive Schwann cells from DRG explants over substantial distances, this growth following the orientation of the suspended fibers as well as that of the perpendicularly positioned supporting walls.…”

Section: Neurite Outgrowth From Explanted Dorsal Root Gangliamentioning

confidence: 95%

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Hrynevich

Achenbach

Jüngst

et al. 2021

Macromolecular Bioscience

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In this study, well-defined, 3D arrays of air-suspended melt electrowritten fibers are made from medical grade poly(ɛ-caprolactone) (PCL). Low processing temperatures, lower voltages, lower ambient temperature, increased collector distance, and high collector speeds all aid to direct-write suspended fibers that can span gaps of several millimeters between support structures. Such processing parameters are quantitatively determined using a "wedge-design" melt electrowritten test frame to identify the conditions that increase the suspension probability of long-distance fibers. All the measured parameters impact the probability that a fiber is suspended over multimillimeter distances. The height of the suspended fibers can be controlled by a concurrently fabricated fiber wall and the 3D suspended PCL fiber arrays investigated with early post-natal mouse dorsal root ganglion explants. The resulting Schwann cell and neurite outgrowth extends substantial distances by 21 d, following the orientation of the suspended fibers and the supporting walls, often generating circular whorls of high density Schwann cells between the suspended fibers. This research provides a design perspective and the fundamental parametric basis for suspending individual melt electrowritten fibers into a form that facilitates cell culture.

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“…introduced a printing system, which integrates extrusion printing and electrospinning for forming structures with micro-nano scale features. The system had a micro-level printing resolution to meet the requirements of forming exquisite complex cross-scale structures[ 76 ]. Liu et al .…”

Section: 3d Composite Bioprinting Systemmentioning

confidence: 99%

3D Composite Bioprinting for Fabrication of Artificial Biological Tissues

Zhang¹,

Wang²,

Jun-chao³

et al. 2024

IJB

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Three-dimensional (3D) bioprinting is an important technology for fabricating artificial tissue. To effectively reconstruct the multiscale structure and multi-material gradient of natural tissues and organs, 3D bioprinting has been increasingly developed into multi-process composite mode. The current 3D composite bioprinting is a combination of two or more printing processes, and oftentimes, physical field regulation that can regulate filaments or cells during or after printing may be involved. Correspondingly, both path planning strategy and process control all become more complex. Hence, thecomputer-aided design and computer-aided manufacturing (CAD/CAM) system that is traditionally used in 3D printing systemis now facing challenges. Thus, the scale information that cannot be modeled in the CAD process should be considered inthe design of CAM by adding a process management module in the traditional CAD/CAM system and add more informationreflecting component gradient in the path planning strategy.

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Structure-induced cell growth by 3D printing of heterogeneous scaffolds with ultrafine fibers

Cited by 113 publications

References 45 publications

Biomimetic Tympanic Membrane Replacement Made by Melt Electrowriting

Biomimetic Tympanic Membrane Replacement Made by Melt Electrowriting

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

3D Composite Bioprinting for Fabrication of Artificial Biological Tissues

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