Structure identification of walnut peptides and evaluation of cellular antioxidant activity

Wang, Jinping; Liu, Junmei; John, Afiya; Jiang, Yueming; Zhu, Hong; Yang, Bao; Wen, Li

doi:10.1016/j.foodchem.2022.132943

Cited by 44 publications

(26 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a previous study has demonstrated that an antioxidant peptide (WLP) derived from the sea squirt effectively suppressed cytotoxicity in 6-Hydroxydopamine-induced PC12 cells by increasing cell viability and decreasing intracellular ROS levels in a dose-dependent manner [ 27 ]. However, not all antioxidant peptides exhibited protective effects, which might be attributed to their structural characteristics, such as amino acid sequence, composition, and hydrophobicity [ 17 , 28 ]. More importantly, SGGY was still nontoxic to SH-SY5Y cells when the concentration was up to 2 mg/mL, which was consistent with the view that food-derived peptides are generally recognized as safe [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of a Novel Tetrapeptide SGGY from Walnut against H2O2-Stimulated Oxidative Stress in SH-SY5Y Cells: Possible Involved JNK, p38 and Nrf2 Signaling Pathways

Feng

Wang

et al. 2023

Foods

View full text Add to dashboard Cite

SGGY, an antioxidant tetrapeptide identified from walnut protein hydrolysate in our previous study, has been suggested to possess the potential to alleviate oxidative stress in cells. In this paper, the neuroprotective effects of SGGY on H2O2-stimulated oxidative stress in SH-SY5Y cells and the underlying mechanisms were investigated. Results showed that SGGY alleviated H2O2-induced oxidative stress by decreasing the intracellular reactive oxygen species (ROS) level and altering the mitochondrial membrane potential (MMP), thereby inhibiting apoptosis and increasing cell viability. SGGY significantly restored antioxidant enzyme activities and reduced malondialdehyde (MDA) content accordingly. Moreover, SGGY promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and suppressed the H2O2-induced activation of JNK and p38 mitogen-activated protein kinases (MAPKs). Taken together, these results suggested that SGGY protected SH-SY5Y cells from H2O2-provoked oxidative stress by enhancing the ability of cellular antioxidant defense, and the possible mechanism involved MAPKs and Nrf2 signaling pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of a Novel Tetrapeptide SGGY from Walnut against H2O2-Stimulated Oxidative Stress in SH-SY5Y Cells: Possible Involved JNK, p38 and Nrf2 Signaling Pathways

Feng

Wang

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…The amino acid composition of treated and untreated soymilk were measured following the protocol of previous report [14] . In brief, 100 mg of freeze-dried soymilk powder were hydrolyzed by 6 M HCl at 110 °C for 23 h. Notely, methionine and cystine were hydrolyzed after pre-treating with performic acid oxidation.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of icariin-soymilk nanoparticles with ultrasound-assisted treatment

Wang

Zhu²,

Jiang³

et al. 2022

Ultrasonics Sonochemistry

Self Cite

View full text Add to dashboard Cite

“…Numerous studies have confirmed that walnut peptides have a strong antioxidant capacity both in vitro and in vivo, which is closely related to the amino acid sequence and composition of the peptides (31,32). Walnut peptides are mostly composed of two to several dozen amino acids through peptide bonds and their relative molecular mass is generally less than 6000 Da (30).…”

Section: Walnut Peptides 321 Functional Properties Of Walnut Peptidesmentioning

confidence: 99%

An overview of walnuts application as a plant-based

Zhou

Pei

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

The plant-based refers to plant-based raw materials or products that are available as the source of protein and fat. Utilization and development of walnuts as a plant-based, resulting in a high-quality protein-rich walnut plant-based product: walnut protein powder and walnut peptides. Progress in research on the application of walnuts as a plant-based has been advanced, solving the problem of wasted resources and environmental pollution caused by the fact that walnut residue, a product of walnuts after oil extraction, is often thrown away as waste, or becomes animal feed or compost. This paper reviews and summarizes the research and reports on walnut plant-based at home and abroad, focusing on the application of walnut plant-based in the preparation process (enzymatic and fermentation methods) and the biological activity of the walnut protein and walnut peptide, to provide a theoretical basis for the further processing of walnuts as a walnut plant-based. It can make full use of walnut resources and play its nutritional and health care value, develop and build a series of walnut plant-based products, improve the competitiveness of walnut peptide products, turn them into treasure, and provide more powerful guidance for the development of food and medicine health industry in Yunnan.

show abstract

Structure identification of walnut peptides and evaluation of cellular antioxidant activity

Cited by 44 publications

References 38 publications

Neuroprotective Effects of a Novel Tetrapeptide SGGY from Walnut against H2O2-Stimulated Oxidative Stress in SH-SY5Y Cells: Possible Involved JNK, p38 and Nrf2 Signaling Pathways

Neuroprotective Effects of a Novel Tetrapeptide SGGY from Walnut against H2O2-Stimulated Oxidative Stress in SH-SY5Y Cells: Possible Involved JNK, p38 and Nrf2 Signaling Pathways

Fabrication of icariin-soymilk nanoparticles with ultrasound-assisted treatment

An overview of walnuts application as a plant-based

Contact Info

Product

Resources

About