Structure-function relationships among highly diverse T cells that recognize a determinant from influenza virus hemagglutinin.

Abstract

SummaryWe have analyzed the structural and genetic basis for T cell recognition of the complex formed between antigen and class II products of the major histocompatibility complex by performing sequence analysis of T cell receptors (TCRs) induced in response to the helper T cell site 1 of the influenza virus hemagglutinin . The results demonstrate, first, that structurally highly diverse TCRs can be utilized in recognition of the same antigen/1-Ed complex: 12 of 13 TCRs utilize unique Vct/VS gene segment combinations, suggesting that -70 different Vtx/V# combinations are available to BALB/c mice in response to this determinant . Second, comparison of these sequences with the ability of each hybridoma to recognize a panel of peptide analogues suggests that a and (3 chains of these TCRs frequently determine specificity for the NH2-terminal and the COOH terminal portions, respectively, of the site 1 determinant .

Analogues of CTL epitopes with improved MHC class-I binding capacity elicit anti-melanoma CTL recognizing the wild-type epitope

“…Previously, it was observed that the repertoire of CTL directed against the MAGE-1 161-169 antigenic tumor peptide (HLA-A*0101) in a melanoma patient can be diverse in terms of both antigen recognition and TCR usage (Romero et al, 1995). Analyses of TCR repertoires specific for a variety of antigenic peptides presented in MHC class-I molecules have shown that they can be either relatively homogeneous (Kalams et al, 1994;Lehner et al, 1995) or highly diverse (Taylor et al, 1990;Cole et al, 1994). Thus far it is not clear what determines the degree of diversity in the TCR repertoires.…”

CD25+ Immunoregulatory CD4 T Cells Mediate Acquired Central Transplantation Tolerance

“…The TCR ␣-and ␤-chain genes cloned to generate TS1 mice were derived from a T cell line specific for the immunodominant epitope of influenza A virus PR8 hemagglutinin (HA) 3 (22,23). As a result, TS1 mice possess a high percentage of T cells specific for the hemagglutinin molecule S1 determinant (aa 110 -119) presented in the context of MHC class II IE d .…”

A new mouse T-cell receptorα chain variable region family

“…The reaction was incubated at 25 °C for 3 min and stopped by the addition of an equal volume of 5 mM ethylenediaminetetraacetate (EDTA; pH 8.0) and extraction with phenol. The reaction products were precipitated twice with ethanol and converted to dsDNA for amplification in the PCR as described (Taylor et al 1990). Sequence analysis of the V~ gene segment from the SJL-HE-1.1 T-cell hybrid required that the V first be cloned into XZap (Stratagene, La Jolla, CA) in order to eliminate the BW5147 fusion partner V~ transcripts.…”

“…Sequence analysis of the V gene segment from the T2.5-5 T-cell clone was performed essentially as previously described (Taylor et al 1990). Constant region specific primers were used to direct one-sided amplification in the polymerase chain reaction (PCR) and the DNA amplification products were subjected to direct sequence analysis.…”