Structure-Function Relationship in Peridinin-Chlorophyll Proteins

Polı́vka, Tomáš; Hofmann, Eckhard

doi:10.1007/978-94-017-8742-0_3

Cited by 6 publications

(18 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As Q y is the target for energy transfer, this is not surprising. Thus, our simple coupling model agrees with the experimental evidence of a strong energy transfer from Per to Q y [9]. Interestingly, the coupling to Q y does not discriminate between the Per states.…”

Section: Dipole-dipole Couplingsupporting

confidence: 87%

“…It has been linked to the BLA parameter and to the ratio of single/double excitation character [16], which is confirmed by our present data (not shown). It should be pointed out that our theoretical results are at odds with some experimentally derived models [9] for the energy transfer in PCP: Apparently, it is not the S 2 (1B u ) state that is shifted to lower wavelengths to facilitate the green absorption, but it is the S 1 (2A g ) state that is shifted up in energy. Considering light harvesting, closing the energy gap between 1B u and 2A g is counterproductive as it limits the available absorption wavelengths, and as such it is likely a price to be paid for the acquisition of 2A g oscillator strength.…”

Section: Ground State Geometriescontrasting

confidence: 47%

“…In this study, we have introduced the protein environment explicitly, enabling us to see its effect on the Per and Chl a dipole moments. The PCP environment in PCP has been considered to be of moderate polarity [9], which should be reflected by our model. The changes of the state dipole moments of Per are listed in Table 2 for various geometries and environments.…”

Section: Dipole Moments Of Per Statesmentioning

confidence: 99%

“…Experimentally, PCP exhibits high, near-100% energy transfer efficiency from the main chromophore Per to Chl a [2,8]. Several pathways have been proposed for this energy transfer, as summarized in a recent review [9].…”

Section: Introductionmentioning

confidence: 99%

“…Femtosecond transient absorption spectroscopy shows that this process involves the formation of an internal charge transfer (ICT) state with a lifetime of 2-3 ps that transfers energy quantitatively into Chl [13]. Several pathways for the formation of the ICT state have been proposed, most of them involving internal conversion (IC) as the basic mechanism [9]; a recent study has provided experimental and theoretical evidence for the direct formation of the ICT state by 1B u /2A g state mixing along the 1B u relaxation path [12].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Vibrational relaxation as the driving force for wavelength conversion in the peridinin–chlorophyll a-protein

Götze

Karasulu

Patil

et al. 2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

View full text Add to dashboard Cite

We present a computationally derived energy transfer model for the peridinin-chlorophyll a-protein (PCP), which invokes vibrational relaxation in the two lowest singlet excited states rather than internal conversion between them. The model allows an understanding of the photoinduced processes without assuming further electronic states or a dependence of the 2Ag state character on the vibrational sub-state. We report molecular dynamics simulations (CHARMM22 force field) and quantum mechanics/molecular mechanics (QM/MM) calculations on PCP. In the latter, the QM region containing a single peridinin (Per) chromophore or a Per-Chl a (chlorophyll a) pair is treated by density functional theory (DFT, CAM-B3LYP) for geometries and by DFT-based multireference configuration interaction (DFT/MRCI) for excitation energies. The calculations show that Per has a bright, green light absorbing 2Ag state, in addition to the blue light absorbing 1Bu state found in other carotenoids. Both states undergo a strong energy lowering upon relaxation, leading to emission in the red, while absorbing in the blue or green. The orientation of their transition dipole moments indicates that both states are capable of excited-state energy transfer to Chl a, without preference for either 1Bu or 2Ag as donor state. We propose that the commonly postulated partial intramolecular charge transfer (ICT) character of a donating Per state can be assigned to the relaxed 1Bu state, which takes on ICT character. By assuming that both 1Bu and 2Ag are able to donate to the Chl a Q band, one can explain why different chlorophyll species in PCP exhibit different acceptor capabilities.

show abstract

Section: Dipole-dipole Couplingsupporting

confidence: 87%

Section: Ground State Geometriescontrasting

confidence: 47%

Section: Dipole Moments Of Per Statesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Vibrational relaxation as the driving force for wavelength conversion in the peridinin–chlorophyll a-protein

Götze

Karasulu

Patil

et al. 2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

View full text Add to dashboard Cite

show abstract

Papillae formation on trichome cell walls requires the function of the mediator complex subunit Med25

et al. 2017

View full text Add to dashboard Cite

The plant cell wall plays an important role in communication, defense, organization and support. The importance of each of these functions varies by cell type. Specialized cells, such as Arabidopsis trichomes, exhibit distinct cell wall characteristics including papillae. To better understand the molecular processes important for papillae deposition on the cell wall surface, we identified the GLASSY HAIR 1 (GLH1) gene, which is necessary for papillae formation. We found that a splice-site mutation in the component of the transcriptional Mediator complex MED25 gene is responsible for the near papillae-less phenotype of the glh1 mutant. The MED25 gene is expressed in trichomes. Reporters for trichome developmental marker genes GLABRA2 (GL2) and Ethylene Receptor2 (ETR2) were not affected in the glh1 mutant. Collectively, the presented results show that MED25 is necessary for papillae formation on the cell wall surface of leaf trichomes and suggest that the Arabidopsis MED25 Mediator component is likely involved in the transcription of a subset of genes that promote papillae deposition in trichomes.

show abstract

Oligomerization state and pigment binding strength of the peridinin‐Chla‐protein

Jiang

Zhang

et al. 2015

FEBS Letters

View full text Add to dashboard Cite

a b s t r a c tThe peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.

show abstract

Structure-Function Relationship in Peridinin-Chlorophyll Proteins

Cited by 6 publications

References 67 publications

Vibrational relaxation as the driving force for wavelength conversion in the peridinin–chlorophyll a-protein

Vibrational relaxation as the driving force for wavelength conversion in the peridinin–chlorophyll a-protein

Papillae formation on trichome cell walls requires the function of the mediator complex subunit Med25

Oligomerization state and pigment binding strength of the peridinin‐Chla‐protein

Contact Info

Product

Resources

About