Structure, expression, chromosomal location and product of the gene encoding ADH1 inPetunia

Abstract

A genomic clone for an alcohol dehydrogenase (Adh) gene has been isolated from Petunia hybrida cv. V30 by screening a Petunia genomic library with a maize Adh1 probe. A combination of RFLP and allozyme segregation data failed to demonstrate which of two Adh loci, both of which map to chromosome 4, was the source of the cloned gene. The product of the cloned genes has been identified unequivocally by a transient expression assay in Petunia protoplasts. We have designated this gene Petunia Adh1. The expression of this gene is tightly regulated in the developing anther, where its gene product is the predominant ADH isozyme. It is anaerobically inducible in roots, stems and leaves of seedlings. The induction of enzyme activity is correlated with induction of Adh1 mRNA.

Induced ADH gene expression and enzyme activity in pollinated pistils of Solanum tuberosum

“…Comparison between transcript levels and ADH activity (Fig. Ib,e) indicates that the ADH activity observed in pollinated pistils is presum ably due to de novo transcription, as has also been de scribed for ADH induction in other plant species' (Gregerson et al 1991;Kadowaki et al 1988;Roussclin et al 1994;Xie and Wu 1989).…”

Alcohol Dehydrogenase Genes & Proteins In Grapevine

“…ADH activity has been reported in germinating seeds (Conley et al 1999), pollen (Gregerson et al 1993, Ingersoll et al 1994, Tadege and Kuhlemeier 1997, van Eldik et al 1997, and stems of tree (Kimmerer and Stringer 1988). Changes in ADH activity or in ADH gene expression have also been reported during the normal ripening process of several fruits (Roe et al 1984, van der Streaten et al 1991, Sun Chen and Chase 1993, Ke et al 1994, Longhurst et al 1994, Bonghi et al 1999, Speirs et al 19982002, Echeverria et al 2004, Manriquez et al 2006.…”

“…The apparent ubiquitous response of ADH to abiotic and biotic stresses should however be related to the fact that in plants, ADH generally belongs to a small multigene family leading (in diploids) to the expression of two or three members (Dennis et al 1984, Dennis et al 1985, Gregerson et al 1993, Longhurst et al 1994, Morton et al 1996. Stress response could thus result from differential expression of ADH isogenes.…”

“…Stress response could thus result from differential expression of ADH isogenes. In fact, some studies have shown that ADH isogenes exhibited distinct spatial and/or temporal expression in response to stress or in developing organs (Bicsak et al 1982, Gregerson et al 1991, Gregerson et al 1993, van Eldik et al 1997.…”

Pinus banksiana has at least seven expressed alcohol dehydrogenase genes in two linked groups

“…Most angiosperms express two or three ADH isozymes (13) and Southern blots generally display a corresponding level of complexity (14,15). Sequence data are available for Adh genes of a number of angiosperm species, but relatively little is known about this gene family in conifers, with the only published sequences being three partial Pinus radiata Adh cDNAs, likely representing two loci (7).…”