Induced ADH gene expression and enzyme activity in pollinated pistils of Solanum tuberosum

Eldik, G.J. van; Ruiter, R.K.; Herpen, M. M. A. van; Schrauwen, J. A. M.; Wullems, G. J.

doi:10.1007/s004970050075

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…, 2005; Gass et al. , 2005), and ADH activity has been shown to be induced by the growing pollen tube in styles of potato (van Eldik et al. , 1997).…”

Section: Adh‐p Functions and Biochemistrymentioning

confidence: 99%

The plant ADH gene family

Strommer¹

2011

The Plant Journal

126

122

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SUMMARYThe structures, evolution and functions of alcohol dehydrogenase gene families and their products have been scrutinized for half a century. Our understanding of the enzyme structure and catalytic activity of plant alcohol dehydrogenase (ADH-P) is based on the vast amount of information available for its animal counterpart. The probable origins of the enzyme from a simple b-coil and eventual emergence from a glutathione-dependent formaldehyde dehydrogenase have been well described. There is compelling evidence that the small ADH gene families found in plants today are the survivors of multiple rounds of gene expansion and contraction. To the probable original function of their products in the terminal reaction of anaerobic fermentation have been added roles in yeast-like aerobic fermentation and the production of characteristic scents that act to attract animals that serve as pollinators or agents of seed dispersal and to protect against herbivores.

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“…, 2005; Gass et al. , 2005), and ADH activity has been shown to be induced by the growing pollen tube in styles of potato (van Eldik et al. , 1997).…”

Section: Adh‐p Functions and Biochemistrymentioning

confidence: 99%

The plant ADH gene family

Strommer¹

2011

The Plant Journal

126

122

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“…Reports that alcohol is present within the pollen tube, and possibly also in stylar tissue, is based on the presence and activity of alcohol dehydrogenases. This suggests that less aerobic, higher ethanol, and carbon dioxide concentrations may exist within the style than on the stigma (Bucher et al 1995;Tadege and Kuhlemeier 1997;van Eldik et al 1997;Porterfield et al 1999;Mellema et al 2002).…”

Section: Introductionmentioning

confidence: 95%

Sodium thioglycollate enhances pollen germination and pollen tube elongation in cruciferous species

Watrud

Brewer

Shiroyama

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Sodium thioglycollate is a reducing agent used in microbiological growth media to enhance the growth of anerobic, microaerophilic, and facultative organisms, and in eukaryotic tissue extraction buffers to inhibit damaging oxidative reactions. Sodium thioglycollate was added to a semi-solid pollen germination medium to evaluate its effects on in vitro pollen germination and pollen tube elongation, based on the assumption that conditions within stylar tissues are less aerobic than in ambient conditions. We observed significant increases in the percent germination and pollen tube elongation of both crop and weedy mustard family species, on a medium containing 2.2 mM sodium thioglycollate. This suggests that sodium thioglycollate may be a useful amendment to semi-solid media and to solutions that are used to study pollen vigor, physiology, or gene expression, and to bioassay sensitivities of different species or genotypes to diverse physical and chemical factors.

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“…Plant ADHs are involved in the development of different organs, stress responses, and metabolite synthesis. For instance, ADH expression occurs in a developmentally regulated manner during flower development 14,15 and fruit ripening, 16−19 where it is induced by various environmental stresses. 20−22 ADHs are also involved in metabolic pathways during seed storage and fungal infection.…”

Section: ■ Introductionmentioning

confidence: 99%

Identification and Functional Analysis of Two Alcohol Dehydrogenase Genes Involved in Catalyzing the Reduction of (Z)-3-Hexenal into (Z)-3-Hexenol in Tea Plants (Camellia sinensis)

Liu

Guo

Zhou

et al. 2022

J. Agric. Food Chem.

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Alcohol dehydrogenase (ADH) is a vital enzyme in the biosynthesis pathway of six-carbon volatiles in plants. However, little is known about its functions in tea plants. Here, we identified two ADH genes (CsADH1 and CsADH2). An in vitro protein expression assay showed that both CsADH1 and CsADH2 proteins can catalyze the reduction of (Z)-3-hexenal into (Z)-3-hexenol. Subcellular localization revealed that both CsADH1 and CsADH2 proteins were predominantly localized in the nucleus and cytosol. CsADH1 had high transcripts in young stems in autumn, while CsADH2 showed extremely high expression levels in stems and roots. The expression of CsADH2 was mainly downregulated under ABA treatment, while CsADH1 and CsADH2 transcripts were significantly lower under MeJA treatment at 12 and 24 h. Under cold treatment, CsADH1 transcripts first decreased and then increased, while CsADH2 demonstrated an almost opposite expression pattern. Notably, CsADH2 was significantly upregulated under simulated Ectropis obliqua invasion. Gene suppression by antisense oligonucleotides (AsODNs) demonstrated that AsODN_ADH2 treatment significantly reduced CsADH2 transcripts and the abundance of (Z)-3-hexenol products. The results indicate that the two CsADH genes may play an important role in response to (a)biotic stresses and in the process of (Z)-3-hexenol biosynthesis.

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Induced ADH gene expression and enzyme activity in pollinated pistils of Solanum tuberosum

Cited by 11 publications

References 23 publications

The plant ADH gene family

The plant ADH gene family

Sodium thioglycollate enhances pollen germination and pollen tube elongation in cruciferous species

Identification and Functional Analysis of Two Alcohol Dehydrogenase Genes Involved in Catalyzing the Reduction of (Z)-3-Hexenal into (Z)-3-Hexenol in Tea Plants (Camellia sinensis)

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