Structure Characterization and Evaluation Potential of Antimicrobial Extracts From Phellinus Linteus Against Skin Infectious Pathogens, Staphylococcus Epidermidis Atcc12228 and Propionibacterium Acnes Dmst14916

Objective: Methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged independently in diverse geographic zones and MRSA and Pseudomonas aeruginosa cause surgical site infections. Nosocomial surveillance in orthopedic surgery wards of the hospital for 16 months is presented.Methods: A total of 621 wound swabs were cultured on blood and MacConkey agar plates for bacteria and Sabouraud dextrose agar for fungi.Results: From 468 bacterial colonies, 98 MRSA and 74 P. aeruginosa strains and 41 fungal strains were isolated, and fungal strains were 13 strains of Aspergillus niger, and 28 strains of Candida albicans. P. aeruginosa and S. aureus strains were susceptible to antibiotics tobramycin, ciprofloxacin, piperacillin, vancomycin, levofloxacin, and amoxyclav. Similarly, A. niger and C. albicans were susceptible to antifungals, amphotericin B (AMB), liposomal AMB, itraconazole, voriconazole, posaconazole, and caspofungin.Conclusion: Isolated MRSA strains were resistant to presently used common antibiotics, which attribute to the leading causatives of post-operative infection in orthopedic wounds, specifically.

Section: Discussionmentioning

confidence: 99%

Surveillance of Bacteria Methicillin-Resistant Staphylococcus Aureus and Pseudomonas Aeruginosa in Patients Admitted to Orthopedic Department in a Tertiary Referral Hospital

Das

Mishra

Rath

2018

“…MICs were determined to evaluate the antimicrobial activity of synthesized compounds [26,27]. A pure culture of a single microorganism was grown in Mueller-Hinton broth (MHB).…”

Section: Antimicrobial Studiesmentioning

confidence: 99%

Indanyl Analogs as Potential Antimicrobial Agents

Mandal¹

2018

Objective: The wide variety of biological activities of different indane derivatives makes them an interesting moiety in the field of medicinal chemistry. The objective of the study was to identify and develop novel antimicrobial agents from indanyl analogs. Methods:Recently synthesized indanyl analogs (4a-c and 5a-o) were examined against various pathogenic microorganisms (Gram-positive and Gram-negative bacteria and fungi) using serial dilution method. These analogs were found to possess antibacterial and antifungal activities with minimum inhibitory concentration values ranging between 1.56 and 100 µg/mL. Results:The results revealed that the entire compounds showed mild-to-moderate antibacterial activities and moderate-to-excellent antifungal activities against the pathogenic microorganisms as compared to the standard drugs ciprofloxacin and fluconazole, respectively. Compounds 4a, 5a, 5b, 5d, 5e, 5i, and 5j exhibited antifungal activities superior to the reference drug. Conclusion:Based on the structure-activity relationship, it can conclude that the indan-3-oxo-1-acetic acid moiety is essential for the activities and lipophilic alkoxy substituents on indane ring have enhanced the biological activity. Further, structure-activity relationship studies of the compounds 4a, 5a, and 5b are needful to find the new lead as antimicrobial agent.

“…The bacterial strains were grown on 5% nutrient broth (NB) and after initial incubation, organisms were suspended in 15 ml of NB and optical density was measured. The readings were compared with 0.5 McFarland standard [11]. For the determination of MIC, bacterial suspension at 5×10 6 colony-forming units (CFU)/ml was employed [12].…”

Section: In Vitro Antibacterial Assaymentioning

confidence: 99%

Bioactive Fraction of Tropidia Curculioides, a Rare Orchid of Arunachal Pradesh, India: Phytochemical Profile and Marker Compounds

Sarkar

Avasthi

Ghosal

2018

Objective: Chemical profiling of the most active fraction of Tropidia curculioides, isolation and characterization of marker compounds along with the evaluation of biological activity. Methods:The most active Et 2 O fraction of roots of T. curculioides (TC) was analyzed by gas chromatography coupled with a mass selective detector. Repetitive chromatography followed by high-performance liquid chromatography of the active fraction afforded three major compounds. The structure of the compounds was established by nuclear magnetic resonance and mass spectral analysis. Antibacterial activity of the compounds was determined by agar well diffusion method against five multidrug-resistant (MDR) clinical isolates while minimum inhibitory concentration (MIC) and minimum bactericidal concentration were determined by microdilution method. Scanning electron microscopy was performed with compound 2 against Escherichia coli cells at MIC (15 µg/ml). Results:Analysis of the gas chromatography-mass spectrometry (GC-MS) spectra revealed that the isolated compounds constituted 27.16% of the total constituents. Two other chemical classes, namely, saturated fatty acids and sterol constituted 38.04% and 12.49%, respectively. The structure of the compounds was characterized as 4-hydroxy benzaldehyde (1), bisphenol F (2), and 3,5-dihydroxy-4-methoxybenzoic acid (3). The most significant bacteriostatic and bactericidal activity against E. coli comparable to that of tetracycline and gentamicin was observed with compound 2. Microscopic study confirmed that compound 2 affects integrity of cell membrane severely, causing death of the bacterium. Compound 1 and 3 showed moderate to good antibacterial activity against E. coli and Enterococcus sp. Cell cytotoxicity of the compounds was well within permissible limit. This is the first report of compounds in TC. Conclusion:The study identified three marker compounds from the less polar fraction of the roots of TC with significant antibacterial activity. The GC-MS spectra with marker compounds could be treated as the chemical fingerprinting of the bioactive fraction. The outcome justifies the use of the plant in traditional medicine.