Structure characterisation of polysaccharides in vegetable “okra” and evaluation of hypoglycemic activity

Liu, Juan; Zhao, Yupeng; Wu, Qifan; John, Afiya; Jiang, Yueming; Yang, Jiali; Liu, Huiling; Yang, Bao

doi:10.1016/j.foodchem.2017.09.051

Cited by 154 publications

(66 citation statements)

References 17 publications

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“…In another investigation, the high dose of rhamnoglacturonan (200 mg/kg) (water-soluble polysaccharides extracted from okra pod) lowered blood glucose and glucose resistance in STZ induced diabetic rats at 5th and 10th day of study. The hypoglycemic effects were similar to positive control group (glimepride as a sulfonylurea antidiabetic medicine) (Liu et al, 2018).…”

Section: Effect Of Okra On Diabetessupporting

confidence: 62%

Effect of Abelmoschus esculentus (okra) on metabolic syndrome: A review

Esmaeilzadeh

Razavi

Hosseinzadeh

2020

Phytotherapy Research

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Metabolic syndrome is a disorder characterized by dyslipidemia, insulin resistance, abdominal fat, high blood pressure, hypertriglyceridemia, and diminished high density lipoprotein cholesterol. Okra (Abelmoschus esculentus L.), routinely called lady's finger, has belonged to the Malvaceae family. Okra is considered as a valuable crop due to the multiple functions of its leaves, buds, flowers, pods, stems, and seeds in traditional and modern medicines. Several bioactive components are presented in different parts of okra including polyphenolic compounds especially oligomeric catechins and flavonol derivatives such as quercetin. The antioxidant, anti‐inflammatory, anticancer, immunomodulatory, gastroprotective, neuroprotective, lipid lowering, and antidiabetic effects of okra have been established. Although different in vivo and in vitro studies revealed that okra has an ability to overcome metabolic syndrome symptoms, the lack of clinical studies is notable. So, further clinical trials should be accomplished to confirm the role of okra in metabolic syndrome. The aims of this review are to gather different studies regarding the potential efficacy of okra in metabolic syndrome.

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Section: Effect Of Okra On Diabetessupporting

confidence: 62%

Effect of Abelmoschus esculentus (okra) on metabolic syndrome: A review

Esmaeilzadeh

Razavi

Hosseinzadeh

2020

Phytotherapy Research

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“…Current research has confirmed that Okra extract can reduce blood glucose (2), consistent with our previous research results. However, since various flavonoids and polysaccharides contained in Okra extract have been confirmed to have hypoglycemic effects (10,11), we propose that some major components of Okra can be studied in vitro so as to further explore their mechanism of reducing blood glucose. Isoquercitrin and quercetin are two major flavonoids of Okra (12).…”

Section: Discussionmentioning

confidence: 99%

Quercetin And Isoquercitrin Inhibiting Hepatic Gluconeogenesis Through Lkb1-Ampka Pathway

et al. 2020

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Objective. To observe the impact of quercetin and isoquercitrin on gluconeogenesis in hepatocytes. Methods. Mouse primary hepatocytes were cultured with lactic acid and pyruvic acid. After treatment with quercetin and isoquercitrin for 24 hours, the glucose concentration in the culture supernatant was determined. RT-PCR was used to detect the mRNAs of PEPCK, G6Pase, LKB1, and AMPKα. Protein levels of LKB1, AMPKα, and Thr172 phosphorylation were evaluated by Western blot. Results. The glucose concentration in the gluconeogenesis group (GN) was significantly higher than in the control group (C), but the glucose concentrations in the high level quercetin(group 80Q) and high level isoquercitrin (group 80I) were significantly lower than in the group GN, P<0.01. In the group 80Q, and group 80I, the mRNA levels of PEPCK and LKB1were significantly lower than in the group GN (P<0.01), and the G6Pase mRNA were significantly lower than in the group GN (P<0.05). The protein levels of LKB1 and the phosphorylation of AMPKα Thr172 in the group 80Q, group 40I, and group 80I were higher than in the group GN. The effects of quercetin and isoquercitrin on LKB1 and AMPKα were similar to those of metformin. Conclusions. Quercetin and isoquercitrin inhibit gluconeogenesis in hepatocytes, which may be related to the LKB1 upregulation and phosphorylation of AMPKα.

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“…Combined with periodate oxidation results, Smith degradation analysis, 2D NMR spectra, and documented data, 1 H and 13 C NMR chemical shifts of the main residues from PSP-1 were shown in Table 3 . The anomeric proton and carbon signals at 5.35/92.17, 5.16/101.50, 4.93/98.08, 4.59/95.87, 4.57/103.64 were assigned to the H-1/C-1 of A: →4)-α- d -Galp-(1→, B: →6)-α- d -Glcp-(1→, C: α- d -Glcp-(1→, D: →3,6)-β- d -Manp-(1→, and E: →6)-β- d -Galp-(1→ residues, respectively [ 34 , 35 , 36 , 37 , 38 ]. Signals at δ 3.65/75.13, 3.36/69.65, 3.84/81.31, 3.41/68.02, and 3.61/62.63 ppm were attributed to C-2/H-2–C-6/H-6 of →4)-α- d -Galp-(1→, respectively [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…Signals at δ 3.81/66.29, 3.50/72.70, 3.54/72.20, 71.66/3.60, and 3.43/75.82 were attributed to C-2/H-2–C-6/H-6 of →3,6)-β- d -Manp-(1→ [ 37 ], respectively. Moreover, δ 3.44/68.02 (H-2/C-2), δ 3.83/73.23 (H-3/C-3), δ 4.02/70.72 (H-4/C-4), δ 3.96/74.22 (H-5/C-5) and δ 3.73/69.66 (H-6/C-6) were assigned to →6)-β- d -Galp-(1→ [ 38 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds

et al. 2018

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In the present study, ultrasound-assisted extraction (UAE) of crude polysaccharides (PSP) from pumpkin seeds was optimized by response surface method (RSM). The polysaccharide yield (2.29 ± 0.14%), which agreed closely with the theoretical predicted value 2.40%, was obtained under the optimal extraction conditions: extraction time 24 min, extraction temperature 50 °C, ultrasonic power 347 W, and liquid to solid ratio 23 mL/g. After further purification by two-step column chromatography, a novel polysaccharide (PSP-1) was isolated from pumpkin seeds. PSP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.00:4.26:5.78 with molecular weight of 3728 g/mol. 1D and 2D NMR spectroscopy analysis revealed that the backbone of PSP-1 was mainly formed by β→6)-β-d-Galp-(1→, →6)-α-d-Glcp-(1→, and →3,6)-β-d-Manp-(1→ with branching at O-3 and O-6 of →3,6)-β-d-Manp-(1→. Branch linkages were composed of α-d-Glcp-(1→ and →4)-α-d-Galp-(1→.

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Structure characterisation of polysaccharides in vegetable “okra” and evaluation of hypoglycemic activity

Cited by 154 publications

References 17 publications

Effect of Abelmoschus esculentus (okra) on metabolic syndrome: A review

Effect of Abelmoschus esculentus (okra) on metabolic syndrome: A review

Quercetin And Isoquercitrin Inhibiting Hepatic Gluconeogenesis Through Lkb1-Ampka Pathway

Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds

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