Structure, chain conformation, and immunomodulatory activity of the polysaccharide purified from Bacillus Calmette Guerin formulation

Liu, Wei; Wang, Hong; Yu, Juping; Liu, Yameng; Lu, Weisheng; Chai, Yin; Liu, Chao; Pan, Chun; Yao, Wenbing; Gao, Xiangdong

doi:10.1016/j.carbpol.2016.05.011

Cited by 99 publications

(37 citation statements)

References 36 publications

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“…182 BCG-PSN can protect against TB infection by regulating cellular and humoral immunity, stimulating the reticuloendothelial system, and activating monocytes and macrophages. 183 A recent study suggested that BCG-PSN could induce high levels of IFN-g and TNF-a in peripheral blood CD4 C T cells from mice receiving BCG-PSN powder delivered via microneedle patch. Treatment improved pathological changes in their lungs and spleens compared to the control group.…”

Section: Aec/bc02mentioning

confidence: 99%

The current status, challenges, and future developments of new tuberculosis vaccines

Gong

Liang

2018

Human Vaccines & Immunotherapeutics

103

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Mycobacterium tuberculosis complex causes tuberculosis (TB), one of the top 10 causes of death worldwide. TB results in more fatalities than multi-drug resistant (MDR) HIV strain related coinfection. Vaccines play a key role in the prevention and control of infectious diseases. Unfortunately, the only licensed preventive vaccine against TB, bacilli Calmette-Guérin (BCG), is ineffective for prevention of pulmonary TB in adults. Therefore, it is very important to develop novel vaccines for TB prevention and control. This literature review provides an overview of the innate and adaptive immune response during M. tuberculosis infection, and presents current developments and challenges to novel TB vaccines. A comprehensive understanding of vaccines in preclinical and clinical studies provides extensive insight for the development of safer and more efficient vaccines, and may inspire new ideas for TB prevention and treatment.

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Section: Aec/bc02mentioning

confidence: 99%

The current status, challenges, and future developments of new tuberculosis vaccines

Gong

Liang

2018

Human Vaccines & Immunotherapeutics

103

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“…The DIP-1's triple-helix conformation could be evaluated using the method of Wei et al (2016) with some modifications. In brief, DIP-1 solution (2.5 mg/ml, 2 ml) was mixed with Congo red reagent (80 μm, 2 ml).…”

Section: Congo Red Testmentioning

confidence: 99%

“…The dye of Congo red was acid and often used to verify a triple-helix conformation of polysaccharides (Chen & Kan, 2017). Compared with Congo red, the maximum absorption wavelength of the polysaccharides complex will be red-shifted (Wei et al, 2016). As could be seen in Figure 4b, the Congo red control was compared with the maximum absorption wavelength of the DIP-1-Congo red complex in increasing concentrations of NaOH solutions ranging from 0.0 to 0.5 M. There was not observed the red shift phenomenon of the sample, indicating that DIP-1 did not own the triple-helical conformation in solution.…”

Section: Congo Red Testmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

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“…GPC chromatograms of CMI with a sharp peak are shown in Figure 4a. The value of dn/dc (mL/g) and polydispersity (Mw/Mn) of CMI were 0.13 and 1.11, respectively, which indicated that CMI was monodisperse [45,46]. When compared with GPC chromatograms of inulin in Figure 4b, the peak of CMI had shorter retention time, which suggested that the molecular of CMI was higher than inulin (2.70 × 10 3 ± 96 Da).…”

Section: Resultsmentioning

confidence: 98%

Formation of Nanocomplexes between Carboxymethyl Inulin and Bovine Serum Albumin via pH-Induced Electrostatic Interaction

Huang

Jin

et al. 2019

Molecules

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As a functional polysaccharide, inulin was carboxymethylated and it formed nanocomplexes with bovine serum albumin (BSA). The success of obtaining carboxymethyl inulin (CMI) was confirmed by a combination of Fourier transform Infrared (FT-IR), Raman spectroscopy, gel permeation chromatography (GPC), and titration. The effects of pH and ionic strength on the formation of CMI/BSA nanocomplexes were investigated. Our results showed that the formation of complex coacervate (pHφ1) and dissolution of CMI/BSA insoluble complexes (pHφ2) appeared in pH near 4.85 and 2.00 respectively. FT-IR and Raman data confirmed the existence of electrostatic interaction and hydrogen bonding between CMI and BSA. The isothermal titration calorimetry (ITC) results suggested that the process of complex formation was spontaneous and exothermic. The complexation was dominated by enthalpy changes (∆Η < 0, ∆S < 0) at pH 4.00, while it was contributed by enthalpic and entropic changes (∆Η < 0, ∆S > 0) at pH 2.60. Irregularly shaped insoluble complexes and globular soluble nanocomplexes (about 150 nm) were observed in CMI/BSA complexes at pH 4.00 and 2.60 while using optical microscopy and atomic force microscopy, respectively. The sodium chloride suppression effect on CMI/BSA complexes was confirmed by the decrease of incipient pH for soluble complex formation (or pHc) and pHφ1 under different sodium chloride concentrations. This research presents a new functional system with the potential for delivering bioactive food ingredients.

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Structure, chain conformation, and immunomodulatory activity of the polysaccharide purified from Bacillus Calmette Guerin formulation

Cited by 99 publications

References 36 publications

The current status, challenges, and future developments of new tuberculosis vaccines

The current status, challenges, and future developments of new tuberculosis vaccines

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Formation of Nanocomplexes between Carboxymethyl Inulin and Bovine Serum Albumin via pH-Induced Electrostatic Interaction

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