Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Abstract

A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

“…The peak at 1024 cm –1 is the characteristic absorption peak of asymmetric stretching vibrations of the ether bond on the sugar ring, indicating that there are pyranose units in RYRO1 (He et al., 2021; You et al., 2021). The signal at 1623 cm –1 was the characteristic absorption of bonded water, indicating the N–H of primary amine (Xiang et al., 2019). The signal at 1398 cm –1 was contributed by the bending vibrations of C–H (Li et al., 2019; Xiang et al., 2019).…”

“…The signal at 1623 cm –1 was the characteristic absorption of bonded water, indicating the N–H of primary amine (Xiang et al., 2019). The signal at 1398 cm –1 was contributed by the bending vibrations of C–H (Li et al., 2019; Xiang et al., 2019). Moreover, the pyranoside ring absorption band was observed at 878 cm –1 (C–H ring vibration) (Nejatzadeh‐Barandozi & Enferadi, 2012) and indicated a β‐glycosidic linkage (Mohammed et al., 2020).…”

Characterization of Alpinia officinarum Hance polysaccharide and its immune modulatory activity in mice

“…SEM is a commonly used method to identify the surface microstructure of polysaccharides. 16 AOHP was observed at high magnifications (2000 and 10 000 folds). Also, it showed fragmented flakes with rough surfaces (Fig.…”

“…The solutions to be tested were prepared according to the method described in a previous study. 16 The final concentration of Congo red was 40 μg mL −1 , and NaOH reached 0.1, 0.2, 0.3, 0.4, and 0.5 M, respectively. Every mixture was maintained for 30 min at room temperature and scanned by a UV spectrophotometer (721 N; Shanghai Yidian, China) in the 400-600 nm range with maximum absorption wavelength transitions at different recorded NaOH concentrations.…”

Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

“…Duchesnea indica , commonly known as false strawberry, is normally found in east Asia, in particular, China and Japan. It has been reported that the leaves and whole plant exhibit anticancer [ 10 , 11 ], antioxidant [ 12 ], and immune modulatory activities [ 13 ]. There have been no studies specifically evaluating the efficacy of Duchesnea indica extract (DIE) as a therapeutic agent for sepsis.…”