Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

Abstract

Objective. Duchesnea indica has been reported for its anti-inflammatory properties. However, its efficacy in sepsis has yet to be reported. In this study, we studied the ability of Duchesnea indica extract (DIE) to rescue mice from septic shock and sepsis. Methods. In vitro studies included the measurement of secreted nitric oxide, cell viability, gene and protein expression via real-time polymerase chain reaction and western blot, and confocal microscopy in RAW 264.7 cells. In vivo studies include a model of septic shock and sepsis in BALB/c mice induced by a lethal and sub-lethal dose of lipopolysaccharide (LPS). Results. DIE suppressed the expression of proinflammatory cytokines induced by LPS and prevented the translocation of NFκB into the nucleus of RAW 264.7 cells. It also prevented reactive oxygen species damage induced by LPS in murine bone marrow-derived macrophages. Models of sepsis and septic shock were established in BALB/c mice and DIE-rescued mice from septic shock. DIE also reversed the increase in tumor necrosis factor-α and nitrite levels in the serum of mice induced with sepsis. DIE also prevented the translocation of NFκB from the cytosol into the nucleus in murine lungs. Histopathological damage induced by sepsis was reversed in the testis, liver, and lungs of mice. Conclusion. In conclusion, DIE is a suitable candidate for development as a therapeutic agent for sepsis.

Antioxidant activity of natural products from medicinal plants

“…This compound has been the secondary metabolite mostly identified in alcoholic extracts of Potentilla indica. In 2022, Lee et al [51] demonstrated that the oral administration of ethanolic extract of the Potentilla indica (100 and 300 mg/kg) in which ellagic acid was identified by UPLC as the major component, ameliorates LPS-induced septic shock in mice and decreases the levels of inflammatory markers in the serum of mice in a chronic sepsis model by suppressing NF-κB translocation to the nucleus of lung cells in a dose-dependent manner. Similarly, in 2008, Zhao and colleagues [34] demonstrated that ethanolic extract of Potentilla indica (whole plant) displayed anti-inflammatory activity in an inflammatory cell model by addition of lipopolysaccharide (LPS, 10 ng/mL) in the RAW264.7 cell line in the presence of 10, 5, and 1 μg/mL concentrations of the extract for 24 h, blocking NF-κB activation.…”

Biosynthesis of Silver Nanoparticles from Duchesnea indica Extracts Using Different Solvents and Their Antibacterial Activity

“…Variations in the extracted DI phytochemicals based on the solvents used and differences in antibacterial properties were previously reported [26]. Moreover, other studies have reported the application of the ethanol extract of DI as a treatment for sepsis and the water extract of DI as a medicinal cosmetic against skin pathogens such as S. aureus and S. epidermidis [39,40]. Overall, the synthesized AgNPs exhibited better antibacterial activity than AgNO3, but their effect on bacteria varied from that of the DI extract.…”