Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength

Iqbal, Atia; Latif, Ayesha; Jabbar, Basit; Ali, M. Tanseer; Ahmed, Mukhtar; Gul, Ambreen; Rao, Abdul Qayyum; Shahid, Ahmad Ali; Husnain, Tayyab

doi:10.1007/s13562-020-00603-7

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…This suggests that NAC genes might be downregulated by the AGL42 repressor protein. Our docking results were consistent with those of previous studies 35 – 37 .…”

Section: Discussionsupporting

confidence: 93%

Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors

Latif

Azam

Shahid

et al. 2022

Sci Rep

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Premature leaf senescence negatively influences the physiology and yield of cotton plants. The conserved IDLNL sequence in the C-terminal region of AGL42 MADS-box determines its repressor potential for the down regulation of senescence-related genes. To determine the delay in premature leaf senescence, Arabidopsis AGL42 gene was overexpressed in cotton plants. The absolute quantification of transgenic cotton plants revealed higher mRNA expression of AGL42 compared to that of the non-transgenic control. The spatial expression of GUS fused with AGL42 and the mRNA level was highest in the petals, abscission zone (flower and bud), 8 days post anthesis (DPA) fiber, fresh mature leaves, and senescenced leaves. The mRNA levels of different NAC senescence-promoting genes were significantly downregulated in AGL42 transgenic cotton lines than those in the non-transgenic control. The photosynthetic rate and chlorophyll content were higher in AGL42 transgenic cotton lines than those in the non-transgenic control. Fluorescence in situ hybridization of the AG3 transgenic cotton line revealed a fluorescent signal on chromosome 1 in the hemizygous form. Moreover, the average number of bolls in the transgenic cotton lines was significantly higher than that in the non-transgenic control because of the higher retention of floral buds and squares, which has the potential to improve cotton fiber yield.

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“…This suggests that NAC genes might be downregulated by the AGL42 repressor protein. Our docking results were consistent with those of previous studies 35 – 37 .…”

Section: Discussionsupporting

confidence: 93%

Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors

Latif

Azam

Shahid

et al. 2022

Sci Rep

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“…Molecular approaches such as PCR were used for confirmation of transgene in advanced generation of cotton variety VH-289 as was done by 17 , 24 . Transgenic cotton plants successfully amplified the DNA fragments of 1.7 kb, 585 bp and 358 bp for Cry1Ac , Cry2A and cp4EPSPS respectively (Fig.…”

Section: Discussionmentioning

confidence: 99%

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

Iqbal

Ali

Ahmed

et al. 2022

Sci Rep

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Stacking multiple genes into cotton crop to cop up multiple biotic stresses such as insects and weeds is a promising tool to save crop from losses. Transgenic cotton variety, VH-289, with double Bt and cp4EPSPS genes under the control of 35S promoter was used for the expression analyses and biosafety studies. The transgenic cotton plants were screened through PCR amplification of fragments, 1.7 kb for Cry1Ac, 582 bp for Cry2A and 250 bp for cp4EPSPS; which confirmed the presence of all genes transformed in transgenic cotton. The Cry1Ac + Cry2A and cp4EPSPS proteins were quantified through ELISA in transgenic cotton plants. The Glyphosate assay performed by spraying 1900 mL per acre of glyphosate Roundup further confirmed complete survival of transgenic cotton plants as compared to the non-transgenic cotton plants and all weeds. Similarly, insect infestation data determined that almost 99% insect mortality was observed in controlled field grown transgenic cotton plants as compared to the non-transgenic control plants. Evaluation of effect of temperature and soil nutrients availability on transgene expression in cotton plants was done at two different cotton growing regions, Multan and Lahore, Pakistan and results suggested that despite of higher temperature in Multan field, an increased level of Cry and cp4EPSPS proteins was recorded due to higher soil organic matter availability compared to Lahore field. Before commercialization of any transgenic variety its biosafety study is mandatory so, a 90 days biosafety study of the transgenic cotton plants with 40% transgenic cottonseeds in standard diet showed no harmful effect on wister rat model when studied for liver function, renal function and serum electrolyte.

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Deep ensemble model for sequence-based prediction of PPI: Self improved optimization assisted intelligent model

Srivastava,

Mall,

Singh

et al. 2024

Multimed Tools Appl

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Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength

Cited by 7 publications

References 37 publications

Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors

Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

Deep ensemble model for sequence-based prediction of PPI: Self improved optimization assisted intelligent model

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