Structure and sequence variation of the canine perforin gene

Neta, Michal; Wen, Xin; Moore, Peter F.; Bienzle, Dorothee

doi:10.1016/j.vetimm.2009.08.012

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…There is only one variant known in dog genomes, despite variable positions in nucleotide coding sequences (Table 2). This observation is in agreement with the results of an analysis of another 54 individuals of Canis lupus familiaris 42 . A basic level of intraspecific variability has also been observed in camels 43 (one or two variants).…”

Section: Discussionsupporting

confidence: 91%

“…There is only one variant known in dog genomes, despite variable positions nucleotide coding sequences (Table This observation is in agreement with the results of an analysis of another 54 individuals of Canis lupus familiaris. 42 A basic level of intraspecific variability has also been observed in camels 43 (one or two variants). Although the Felidae mostly show comparable levels of protein polymorphism with one to two allelic variants (Table 3), there are species with more variants, such as Caracal caracal (three) and Prionailurus bengalensis (four; see File S8).…”

Section: Discussionmentioning

confidence: 94%

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The deadly face of felid killer cells: The cytotoxic proteins and their genes

Futas

Oppelt

Vychodilova

et al. 2022

HLA

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Natural killer cells and cytotoxic T lymphocytes are the main cell populations of the immune system able to directly kill target cells via cytotoxic granules. Different mammalian species may differ in specific features of their pore‐forming protein (perforin) and granule‐bound serine proteases (granzymes). One perforin gene (PRF1) and four genes encoding granzymes A, B, H, and K (GZMA, GZMB, GZMH, GZMK) were identified in the reference genomes of felids. The objective of this work was to characterize the genes PRF1, GZMA and GZMB in a panel of 17 felid species by next‐generation re‐sequencing. A search of available felid genomes (17 species) retrieved the coding sequences of these genes for comparison to our data. Both sets of sequences or their combinations (23 species) were used for phylogenetic and selection analyses. Nucleotide PRF1, GZMA and GZMB sequences showed high similarities between felid species (over 95% identity). All trees derived from coding sequences expressed phylogenetic relationships corresponding to the zoological taxonomy of the Felidae, except GZMA. No effects of positive selection were detected in the genes studied, however, effects of purifying selection were observed for PRF1 and GZMA. The conservation of PRF1 is in agreement with its critical biological function. The differentiation observed between granzyme sub‐families may reflect an adaptation to pathogen variation. The need to maintain important gene functions and at the same time cope with various pathogens may lead to an equilibrium between positive and negative selective pressures acting on GZMB. The within‐species variability in wild felid populations merits further investigation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

The deadly face of felid killer cells: The cytotoxic proteins and their genes

Futas

Oppelt

Vychodilova

et al. 2022

HLA

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“…Primers specific for ferret IFN-γ and TNF-α were designed based on published sequences (Svitek & von Messling, 2007). To design primers specific for ferret perforin, we used primers specific for the canine perforin gene (Neta et al, 2010) and amplified the ferret perforin gene fragment from RNA isolated from ferret PMBCs. The nucleotide sequence of this fragment was then determined and used to design primers specific for ferret perforin.…”

Section: Methodsmentioning

confidence: 99%

Intravenous Immunoglobulin Protects Against Severe Pandemic Influenza Infection

Rockman

Lowther

Camuglia³

et al. 2017

EBioMedicine

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Influenza is a highly contagious, acute, febrile respiratory infection that can have fatal consequences particularly in individuals with chronic illnesses. Sporadic reports suggest that intravenous immunoglobulin (IVIg) may be efficacious in the influenza setting. We investigated the potential of human IVIg to ameliorate influenza infection in ferrets exposed to either the pandemic H1N1/09 virus (pH1N1) or highly pathogenic avian influenza (H5N1). IVIg administered at the time of influenza virus exposure led to a significant reduction in lung viral load following pH1N1 challenge. In the lethal H5N1 model, the majority of animals given IVIg survived challenge in a dose dependent manner. Protection was also afforded by purified F(ab′)2 but not Fc fragments derived from IVIg, supporting a specific antibody-mediated mechanism of protection. We conclude that pre-pandemic IVIg can modulate serious influenza infection-associated mortality and morbidity. IVIg could be useful prophylactically in the event of a pandemic to protect vulnerable population groups and in the critical care setting as a first stage intervention.

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Monocytes and Macrophages and Their Disorders

Souza¹,

Eren²

2022

Schalm's Veterinary Hematology

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Structure and sequence variation of the canine perforin gene

Cited by 3 publications

References 33 publications

The deadly face of felid killer cells: The cytotoxic proteins and their genes

The deadly face of felid killer cells: The cytotoxic proteins and their genes

Intravenous Immunoglobulin Protects Against Severe Pandemic Influenza Infection

Monocytes and Macrophages and Their Disorders

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