Structure and interaction of<i>Corynebacterium pseudotuberculosis</i>cold shock protein A with Y‐box single‐stranded<scp>DNA</scp>fragment

Caruso, Ícaro Putinhon; Panwalkar, Vineet; Coronado, Mônika A.; Dingley, Andrew J.; Cornélio, Marinônio Lopes; Willbold, Dieter; Arni, Raghuvir K.; Eberle, Raphael J.

doi:10.1111/febs.14350

Cited by 6 publications

(3 citation statements)

References 70 publications

(213 reference statements)

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“…Overexpression of SP_2655 can significantly repress spinosyn αd production in the mutant S. pogona ‐Δ ura4‐ Δc14; however, the production of spinosyn αd increased significantly after SP_2655 was deletion in wild‐type strain, indicating that SP_2655 is a TetR family transcriptional factor that negatively regulates spinosyn αd biosynthesis. As another regulatory factor, cold shock protein A is an RNA chaperone that binds single‐stranded nucleic acids and destabilizes stem loop structures in nascent mRNA at low temperature, thus allowing transcription of downstream genes to continue under cold stress conditions (Wang et al ., 2014 ; Wang et al ., 2016 ; Caruso et al ., 2018 ). Whether deletion of SP_2649 can also cause related phenotypic changes in S. pogona needs to be further verified by genetic modification.…”

Section: Discussionmentioning

confidence: 99%

Deletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl‐spinosyn biosynthesis and growth development

Rang

Cao

et al. 2020

Microbial Biotechnology

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Butenyl-spinosyn, a promising biopesticide produced by Saccharopolyspora pogona, exhibits stronger insecticidal activity and a broader pesticidal spectrum. However, its titre in the wild-type S. pogona strain is too low to meet the industrial production requirements. Deletion of non-target natural product biosynthetic gene clusters resident in the genome of S. pogona could reduce the consumption of synthetic precursors, thereby promoting the biosynthesis of butenyl-spinosyn. However, it has always been a challenge for scientists to genetically engineer S. pogona. In this study, the Latour gene knockout system (linear DNA fragment recombineering system) was established in S. pogona. Using the Latour system, a hybrid NRPS-T1PKS cluster (~20 kb) which was responsible for phthoxazolin biosynthesis was efficiently deleted in S. pogona. The resultant mutant S. pogona-Dura4-Dc14 exhibited an extended logarithmic phase, increased biomass and a lower glucose consumption rate. Importantly, the production of butenyl-spinosyn in S. pogona-Dura4-Dc14 was increased by 4.72-fold compared with that in the wild-type strain. qRT-PCR analysis revealed that phthoxazolin biosynthetic gene cluster deletion could promote the expression of the butenyl-spinosyn biosynthetic gene cluster. Furthermore, a TetR family transcriptional regulatory gene that could regulate the butenyl-spinosyn biosynthesis has been identified from the phthoxazolin biosynthetic gene cluster. Because dozens of natural product biosynthetic gene clusters exist in the genome of S. pogona, the strategy reported here will be used to further promote the production of butenylspinosyn by deleting other secondary metabolite synthetic gene clusters.

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Section: Discussionmentioning

confidence: 99%

Deletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl‐spinosyn biosynthesis and growth development

Rang

Cao

et al. 2020

Microbial Biotechnology

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“…Short simulations of the Corynebacterium pseudotuberculosis cold shock protein A bound to an aptamer molecule were performed by Caruso [ 109 ]. They have shown that ssDNA can also rely on intermittent nonspecific hydrogen bond interactions, in addition to the salt-bridges, to confer complex stability [ 109 ]. Interactions between aromatic amino acids and the free nucleic acid bases contributed to an overall enthalpy energy gain.…”

Section: Molecular Dynamics Simulations Applied To Nucleic Acids (mentioning

confidence: 99%

Molecular Modeling Applied to Nucleic Acid-Based Molecule Development

et al. 2018

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Molecular modeling by means of docking and molecular dynamics (MD) has become an integral part of early drug discovery projects, enabling the screening and enrichment of large libraries of small molecules. In the past decades, special emphasis was drawn to nucleic acid (NA)-based molecules in the fields of therapy, diagnosis, and drug delivery. Research has increased dramatically with the advent of the SELEX (systematic evolution of ligands by exponential enrichment) technique, which results in single-stranded DNA or RNA sequences that bind with high affinity and specificity to their targets. Herein, we discuss the role and contribution of docking and MD to the development and optimization of new nucleic acid-based molecules. This review focuses on the different approaches currently available for molecular modeling applied to NA interaction with proteins. We discuss topics ranging from structure prediction to docking and MD, highlighting their main advantages and limitations and the influence of flexibility on their calculations.

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“…CspA family proteins are small RNA binding proteins that are involved in bacterial response to environmental changes ( 30 – 32 ). P. aeruginosa harbors five CspA family proteins, namely, CspC (PA0456), PA0961, PA1159, CspD (PA2622), and CapB (PA3266) ( 33 ).…”

Section: Introductionmentioning

confidence: 99%

Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of rsaL in Pseudomonas aeruginosa

Gong

Yin

et al. 2022

mBio

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Structure and interaction ofCorynebacterium pseudotuberculosiscold shock protein A with Y‐box single‐strandedDNAfragment

Abstract: Resonance assignment and structural data are available in the Biological Magnetic Resonance Data Bank and Protein Data Bank under accession number 26802 and 5O6F, respectively.

Cited by 6 publications

References 70 publications

Deletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl‐spinosyn biosynthesis and growth development

Deletion of a hybrid NRPS‐T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl‐spinosyn biosynthesis and growth development

Molecular Modeling Applied to Nucleic Acid-Based Molecule Development

Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of rsaL in Pseudomonas aeruginosa

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